Fish trypsin ELISA Kit

Code CSB-EL018811FI
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name protease, serine, 1 (trypsin 1)
Alternative Names Trypsin-1 ELISA kit; EC ELISA kit; Trypsin I ELISA kit
Abbreviation PRSS1
Uniprot No. P35031
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of fish trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:20 Average % 101
Range % 95-105
1:40 Average % 91
Range % 84-95
1:80 Average % 106
Range % 100-111
1:160 Average % 104
Range % 97-108
The recovery of fish trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 87-100
EDTA plasma (n=4) 102 96-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
20 0.231 0.227 0.229
10 0.342 0.337 0.340
5 0.497 0.482 0.490
2.5 0.663 0.645 0.654
1.25 0.932 0.918 0.925
0.625 1.300 1.246 1.273
0.312 1.774 1.724 1.749
0 2.434 2.389 2.412
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Subcellular Location Secreted, extracellular space
Protein Families Peptidase S1 family
Database Links

KEGG: sasa:100137021

UniGene: Ssa.38456

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