Goat follicle-stimulating hormone,FSH ELISA Kit

Code CSB-E13277G
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
follicle stimulating hormone, beta polypeptide
Alternative Names
FSHBFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit
Abbreviation
FSHB
Uniprot No.
Species
Capra hircus (Goat)
Sample Types
serum, plasma
Detection Range
20 mIU/mL-800 mIU/mL
Sensitivity
20 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 87-108  
1:2 Average % 88  
Range % 82-98  
1:4 Average % 95  
Range % 85-105  
1:8 Average % 93  
Range % 84-100  
Recovery
The recovery of goat FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-96  
EDTA plasma (n=4) 92 87-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average    
800 0.138 0.132 0.135    
400 0.226 0.241 0.234    
184 0.343 0.320 0.332    
60 0.610 0.588 0.599    
20 1.241 1.215 1.228    
0 2.638 2.607 2.623    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Goat FSHB ELISA Kit was designed for the quantitative measurement of Goat FSHB protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 20 mIU/mL-800 mIU/mL and the sensitivity is 20 mIU/mL.

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Target Background

Function
(From Uniprot)
Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit beta family
Database Links

UniGene: Chi.38676

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