Guinea pig Substance P(SP)ELISA Kit

Code CSB-E17137Gp
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name pig Substance P(SP)
Alternative Names N/A
Abbreviation SP
Species Cavia porcellus (Guinea pig)
Sample Types serum, plasma
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %94
Range %85-106
1:2Average %89
Range %82-100
1:4Average %94
Range %83-102
1:8Average %95
Range %81-102
The recovery of guinea pig SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-93
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
30002.293 2.211 2.252 2.158
15001.974 1.952 1.963 1.869
7501.506 1.543 1.525 1.431
3751.059 1.032 1.046 0.952
187.50.602 0.593 0.598 0.504
940.377 0.384 0.381 0.287
470.263 0.251 0.257 0.163
00.096 0.092 0.094
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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