Guinea pig Substance P(SP)ELISA Kit

Code CSB-E17137Gp
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
pig Substance P(SP)
Alternative Names
N/A
Abbreviation
SP
Species
Cavia porcellus (Guinea pig)
Sample Types
serum, plasma
Detection Range
47 pg/mL-3000 pg/mL
Sensitivity
11.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %85-106
1:2Average %89
Range %82-100
1:4Average %94
Range %83-102
1:8Average %95
Range %81-102
Recovery
The recovery of guinea pig SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-93
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30002.293 2.211 2.252 2.158
15001.974 1.952 1.963 1.869
7501.506 1.543 1.525 1.431
3751.059 1.032 1.046 0.952
187.50.602 0.593 0.598 0.504
940.377 0.384 0.381 0.287
470.263 0.251 0.257 0.163
00.096 0.092 0.094
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Guinea pig Substance P (SP) ELISA Kit was designed for the quantitative measurement of Guinea pig Substance P (SP) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.8 pg/mL.

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