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Mouse Substance P,SP ELISA Kit

Citations (2) Protocol MSDS
Code CSB-E08359m
Size 96T,5×96T,10×96T
Price Request a Quote
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Product Details

Target Name
Substance P,SP
Associated names
N/A
Abbreviation
SP
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:100Average %96
Range %92-104
1:200Average %94
Range %89-98
1:400Average %89
Range %86-96
1:800Average %94
Range %89-97
Recovery
The recovery of mouse SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)8682-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.615 2.514 2.565 2.381
1001.887 1.786 1.837 1.653
501.093 1.073 1.083 0.899
250.668 0.657 0.663 0.479
12.50.436 0.447 0.442 0.258
6.250.311 0.325 0.318 0.134
3.120.241 0.246 0.244 0.060
00.180 0.187 0.184 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Mouse Substance P (SP) ELISA Kit was designed for the quantitative measurement of Mouse Substance P (SP) protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

Citations

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Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 4 reviews

Submit a Review here

Sample type: Serum

Sample species: Mouse

Sample dilution: 1:200

Review: I use the CSB - normal serum E08359m detection, the od value for 0.7/0.604/0.559/0.639/0.577/0.671

By Anonymous

Sample type: Serum

Sample species: Mouse

Sample dilution: 1:200

Review: I used CSB-E08359m to detect normal serum. The OD value of the standard curve was 2.8995, 2.483, 1.703, 0.9295, 0.532, 0.342, 0.177, 0.0975, and the OD value of the sample (the OD of the sample can provide a general range) was 0.6 to 1.0, and the product was good.

By Anonymous

Sample type: Serum Tissue homogenate Intestine

Sample species: Mouse

Sample dilution: : 1:200

Review: I used CSB-E08359m to detect serum and intestinal tissue homogenate samples from the normal group and model group of mice. The OD values of the standard curve ranged from 1.77 to 0.067, and the OD values of the samples ranged from 0.13 to 1.68. The detection results were all within the standard curve range, and I am satisfied with the product results.

By Anonymous

Sample type: Serum

Sample species: Mouse

Sample dilution: No dilution

Review: I used the CSB-E20037142 to test normal serum. The OD values of the standard curve are 2.8995, 2.483, 1.703, 0.9295, 0.532, 0.342, 0.177, 0.0975, and the OD values of the samples are between 0.3 and 0.5. It works very well and is trustworthy.

By Anonymous

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The Emerging Immune Checkpoint CD160 is Transforming the Landscape of Tumor Immunotherapy Beyond PD-1: CD96 is Becoming the Next "Battleground" in Immunotherapy ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments
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