Human Substance P,SP ELISA Kit

Code CSB-E08357h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Substance P,SP
Alternative Names
N/A
Abbreviation
SP
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %92-100
1:2Average %102
Range %98-105
1:4Average %89
Range %85-96
1:8Average %104
Range %100-108
Recovery
The recovery of human SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8682-92
EDTA plasma (n=4)8581-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.439 2.489 2.464 2.290
2001.933 1.978 1.956 1.782
1001.285 1.298 1.292 1.118
500.799 0.782 0.791 0.617
250.475 0.468 0.472 0.298
12.50.376 0.394 0.385 0.211
6.250.316 0.308 0.312 0.138
00.175 0.173 0.174  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This human substance P (SP) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human SP in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of SP bound in the initial step.

Substance P is well-known for its function as a neurotransmitter and a modulator of pain perception by altering cellular signaling pathways. It is released from sensory nerve endings and is widely present in nerve fibers. Substance P is involved in gastrointestinal functioning, memory processing, angiogenesis, vasodilation, and cell proliferation. It also acts on bones and related tissues by binding to receptors, thus regulating bone metabolism, cartilage metabolism, and fracture healing.

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