Human β-defensins 1 ELISA Kit

Code CSB-E14186h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human DEFB1 ELISA Kit was designed for the quantitative measurement of Human DEFB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

Target Name defensin, beta 1
Alternative Names BD 1 ELISA Kit; BD-1 ELISA Kit; BD1 ELISA Kit; beta 1 ELISA Kit; Beta defensin 1 ELISA Kit; Beta-defensin 1 ELISA Kit; DEFB 1 ELISA Kit; DEFB1 ELISA Kit; DEFB1_HUMAN ELISA Kit; DEFB101 ELISA Kit; Defensin ELISA Kit; Defensin beta 1 ELISA Kit; Defensin beta 1 preproprotein ELISA Kit; HBD 1 ELISA Kit; hBD-1 ELISA Kit; HBD1 ELISA Kit; MGC51822 ELISA Kit
Abbreviation DEFB1
Uniprot No. P60022
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Microbiology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 86  
Range % 80-90  
1:10 Average % 96  
Range % 91-100  
1:20 Average % 97  
Range % 91-105  
1:40 Average % 92  
Range % 86-98  
Recovery
The recovery of human β-defensins 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-99  
EDTA plasma (n=4) 90 85-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.544 2.520 2.532 2.412  
100 1.879 1.760 1.820 1.700  
50 1.165 1.084 1.125 1.005  
25 0.678 0.639 0.659 0.539  
12.5 0.443 0.424 0.434 0.314  
6.25 0.325 0.309 0.317 0.197  
3.12 0.198 0.189 0.194 0.074  
0 0.123 0.117 0.120    
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BD-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled BD-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.
Gene References into Functions
  1. The allelic distribution of the polymorphism rs12355840 in miRNA202 was associated with caries experience in the Brazilian Manaus group. In the Ribeirao Preto group, the allelic and genotypic distributions in the polymorphism rs11362 in DEFB1 were associated with caries experience. PMID: 29727722
  2. These studies identified SNPs that were cis-eQTLs for DEFB1 and AHR and, which were associated with variation in plasma KYN concentrations that were related to severity of major depressive disordersymptoms. PMID: 29317604
  3. a considerable connection of DEFB1 and TCF7L2 gene polymorphisms with nephrolithiasis PMID: 29959006
  4. identification of critical promoter regions of DEFB1 that are epigenetically influenced by lactobacilli in vaginal keratinocytes PMID: 28508168
  5. Endometrial tissues and epithelial cells constitutively secreted both BD-1 and -2 mostly at the apical compartment. Both genistein and daidzein induced BDs secretion which reached a peak at 5-15 min. The apical secretion of BDs was coincidence with increased Cl- secretion induced by genistein. PMID: 29905453
  6. The polymorphisms in DEFB1 were not associated with hBD salivary level and caries experience (p > 0.05) in children. PMID: 28343232
  7. These findings suggest a role for hBD-1 in psoriasis pathogenesis PMID: 28067065
  8. Significant associations were found between periodontitis and g. -20G> A (rs11362) and g. -44C> G (rs1800972) SNPs in DEFB1 gene as well as p.Ala29Thr (rs1126477) and p.Lys47Arg (rs1126478) SNPs in LTF gene. PMID: 28485077
  9. hBD-1 potentiates the induction of in vitro osteoclastogenesis by RANKL via enhanced phosphorylation of the p44/42 MAPKs. PMID: 28709835
  10. findings lead us to hypothesize an involvement of hBD-1 mediated innate immunity in the modulation of the susceptibility towards adeno-tonsillar hypertrophy development. PMID: 29501294
  11. Two DEFB1 SNPs were significantly associated with the DMFT(decayed, missing, filled teeth) index. PMID: 27846636
  12. Multivariate logistic regression analysis showed that hBD-1 basal cell loss (>/=20% of prostatic glands in total cores) is an independent factor for predicting prostatic adenocarcinoma (odds ratio: 4.739, confidence interval: 1.093-20.554, p = 0.038). hBD-1 loss of basal cells is a useful indicator to identify extremely high-risk patients with initially negative biopsy. PMID: 28885732
  13. It was concluded that bile acids can differentially regulate colonic epithelial beta-defensin expression and secretion; the authors discuss the implications for intestinal health and disease.- PMID: 28487283
  14. data provide important insight into the in vivo kinetics of HBD expression, the mechanism of HBD1 induction by HIV-1, and the role of HBDs in the early innate response to HIV-1 during acute infection PMID: 28253319
  15. CpG methylation frequencies in the DEFB1 low CpG-content promoter were significantly higher in the prostate malignant tissues than in adjacent benign tissues. PMID: 27835705
  16. DEFB1 - genotypes were correlated with the hBD-1 salivary concentration. PMID: 27770642
  17. Coding missense and nonsense single nucleotide polymorphisms result in less active HBD1 antibacterial activity against Escherichia coli. PMID: 27160989
  18. A significant difference was found between the levels of staining of TLR-2, TLR-4 and hBD-1 in different lesions from the epidermis, inflammation region, dermis and skin appendages (p < 0.05) in patients with acne vulgaris. PMID: 26695933
  19. Expression of hBD-1, hBD-2, hBD-3, and hBD-4 in healthy and chronic periodontitis gingiva. PMID: 26874342
  20. The -44 CC genotype of the beta-defensin-1 gene (DEFB1 rs1800972) may be associated with susceptibility to chronic periodontitis in Japanese. PMID: 24276427
  21. No clear association was found between the DEFB1 polymorphisms and tuberculosis. PMID: 26991287
  22. DEFB1 polymorphisms alone may not influence pathology susceptibility, however they could possibly concur, together with other factors involved in the genetic control of innate immune system, in the predisposition towards recurrent tonsillitis PMID: 26968045
  23. beta-defensin-1 production is dysregulated in the epithelium of patients with chronic obstructive pulmonary disease PMID: 26568662
  24. dental plaque amount, lactobacilli count, age, and saliva buffer capacity, as well as DEFB1 gene polymorphism, explained a total of 87.8% of variations in DMFT scores. PMID: 26377569
  25. Our meta-analysis provides evidence that DEFB1 genetic polymorphisms rs11362G>A, rs1800972C>G and rs1799946G>A are important contributing factors to the development of digestive diseases PMID: 26232989
  26. Association of infectious pathology of upper respiratory tract and development of periodontitis diseases with markers in DEFB1 (-44G/C) and TLR2 Arg753Gln and Arg677Trp genes was determined. PMID: 27029120
  27. Genotypes and alleles of the DEFB1 gene found in long-living individuals can be considered as the factors that increase the probability of longevity and favorable course of age-related diseases. PMID: 26028230
  28. there is a significant link between innate immunity deregulation through disruption of cationic peptides (hBDs) and the potential development of colon cancer. PMID: 26038828
  29. Possible association between DEFB1 polymorphisms and in vivo salivary levels of hBD-1 in an Italian population of 40 healthy individuals, finding a genotype-related different expression with two of these polymorphisms: -52G > A and -44C > G. PMID: 25939140
  30. We found that RNA interference-induced Caspase-12 silencing increased NOD1, hBD1 and hBD2 expression PMID: 25503380
  31. A-dependent DEFB1 upregulation below 20 mM predicts in vitro antimicrobial activity as well as glucose- and AA-dependent CAMP and IFNG upregulation. PMID: 25815330
  32. We conclude that plant-made recombinant beta-defensins (phBD-1 and phBD-2) are promising antimicrobial substances and have the potential to become additional tools against salmonellosis, particularly when used in combination. PMID: 25417183
  33. The DEFB1 c.-44C>G polymorphism of the GG protective genotype was much less frequent among multiple sclerosis patients than among the controls. PMID: 26434201
  34. beta defensin 1 gene -20G/A, -44C/G and -52G/A single nucleotide polymorphisms were not associated with chronic tonsillitis. PMID: 25683590
  35. Our results suggest the involvement of DEFB1 polymorphisms, in particular that of the c.5*G>A SNP, in the susceptibility of women from Northeast Brazil to HPV infection. PMID: 25410996
  36. Functional polymorphisms in IL1B and DEFB1 influence susceptibility to mold infection in solid-organ transplant recipients. PMID: 25398456
  37. DEFB1 minor haplotypes were also associated with an increased risk of developing Atypical Squamous Cells of Undetermined Significance lesions, being significantly more frequent in HPV negative women with lesions, than without lesions. PMID: 24435641
  38. The present finding explains a common defect in male infertility associated with both asthenozoospermia and leukocytospermia, indicating a dual role of DEFB1 in defending male fertility. PMID: 25122636
  39. GG genotype of C-44G SNP in DEFB1 gene may result in decreased defensin beta-1 production in diabetes. PMID: 25083086
  40. These findings associate DEFB1 5'UTR polymorphisms with HIV-1/AIDS in Mexican women for the first time. PMID: 25001249
  41. hBD-1 suppresses tumor migration and invasion of oral squamous cell carcinoma. PMID: 24658581
  42. Data indicate that CCL2 and IL-10 released from burn patient lineage-CD34+CD31- cells were shown to be responsible for their inhibitory activities on the production of beta-defensin 1 (HBD-1) by epidermal keratinocytes. PMID: 24498256
  43. A possible involvement of DEFB1 gene in inflammatory bowel diseases. PMID: 24034632
  44. genetic variant rs11362 in DEFB 1 influences caries susceptibility in CL/P children. The results support the hypothesis that expression of DEFB 1 in saliva may serve as a biomarker for future caries risk PMID: 23964634
  45. This gene encodes a peptide with antimicrobial activity against S. aureus and H. influenzae. PMID: 20100591
  46. HBD-1 serum levels in chronic kidney disease patients are inversely related to estimated glomerular filtration rate. PMID: 24356550
  47. Our findings suggest interplay between hBD-1 and neuroimmunological responses in Alzheimer's disease PMID: 24139179
  48. IRF5, but not TLR4, DEFEB1, or VDR, is associated with the risk of ulcerative colitis in a Han Chinese population. PMID: 23971939
  49. Data suggest that levels of DEFB1 and DEFB2 are highest in colostrum and decrease in human milk over course of lactation; abundance of DEFB1 in colostrum may have protective against upper respiratory infection in infants up to 6 months. PMID: 24124699
  50. The salivary beta-defensin concentration was significantly higher in patients with oral mucosal diseases than in healthy volunteers; furthermore, in patients, the concentration was significantly higher before treatment than after treatment. PMID: 24564045

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Subcellular Location Secreted. Membrane.
Protein Families Beta-defensin family
Tissue Specificity Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level).
Database Links

HGNC: 2766

OMIM: 602056

KEGG: hsa:1672

STRING: 9606.ENSP00000297439

UniGene: Hs.32949

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