Human 8-Hydroxydeoxyguanosine (8-OHdG) ELISA kit

Human 8-Hydroxydeoxyguanosine (8-OHdG) ELISA kit

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8-OHdG ELISA Kit

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Human 8-Hydroxydeoxyguanosine (8-OHdG) ELISA kit

Specification

Code

CSB-E10140h

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Lead Time

3-5 working days

Abbreviation

8-OHdG

Target Name

8-Hydroxydeoxyguanosine (8-OHdG)

Species

Homo sapiens (Human)

Sample Types

serum, urine, tissue homogenates

Detection Range

2 ng/mL-800 ng/mL

Sensitivity

0.8 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-100
1:2	Average %	95
	Range %	89-102
1:4	Average %	93
	Range %	88-99
1:8	Average %	95
	Range %	89-101

Recovery

The recovery of human 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	96	88-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2	1.379	1.380	1.380
8	0.586	0.576	0.581
40	0.308	0.314	0.311
200	0.137	0.148	0.143
800	0.078	0.075	0.077

References

Chronic occupational exposure to hexavalent chromium causes DNA damage in electroplating workers /,BMC Public Health,2011 11:224
FOXO1 Overexpression Attenuates Tubulointerstitial Fibrosis and Apoptosis in Diabetic Kidneys by Ameliorating Oxidative Injury via TXNIP-TRX Linlin Ji, et al,Oxidative medicine and cellular longevity,March 6
Effect of Daily Consumption of Probiotic Yogurt on Oxidative Stress in Pregnant Women: A Randomized Controlled Clinical Trial /,Nutrition & Metabolism,2012 60(1):62-8.
Associations of neonatal lead, cadmium, chromium and nickel co-exposure with DNA oxidative damage in an electronic waste recycling town Ni W et al,Sci Total Environ,2013 Nov 29;472C:354-362.
Evaluation of Oxidative DNA Damage and Its Relation with Anthropometric Indices in Healthy Adolescents Farzaneh Bagherzadeh1 et al,International Journal of Nutrition and Food Sciences,2015 ; 3(2-2): 21-25
Tumor Necrosis Factor-Alpha and 8-Hydroxy-2'-Deoxyguanosine are Associated with Elevated Urinary Angiopoietin-2 Level in Type 2 Diabetic Patients with Albuminuria He F.-F. et al,Kidney Blood Press Res,2015 40(4):355-65.
Tumor Necrosis Factor-Alpha and 8-Hydroxy-2'-Deoxyguanosine are Associated with Elevated Urinary Angiopoietin-2 Level in Type 2 Diabetic Patients with Albuminuria. He FF.et al,Kidney Blood Press Res.,40(4):355-65
PRDX6 Protects ARPE-19 Cells from Oxidative Damage via PI3K/AKT Signaling X Zha. et al,Cell Physiol Biochem,2015 36:2217-2228
B-glucans: ex vivo inflammatory and oxidative stress results after pasta intake. Barera A.et al,Immun Ageing.,13(1): 1.
Genotoxic risk of quinocetone and its possible mechanism in in vitro studies Wang X.et al,Toxicology Research,2016 5 446-460
Mechanisms of housedust-induced toxicity in primary human corneal epithelial cells: Oxidative stress, proinflammatory response and mitochondrial dysfunction. Xiang P. et al,Environ Int.,2016 ,89-90:30-37.
Molecular mechanisms of dust-induced toxicity in human corneal epithelial cells: Water and organic extract of office and house dust. Xiang P.et al,Environ Int.,92-93:348-56
Hesperidin Supplementation Alleviates Oxidative DNA Damage and Lipid Peroxidation in Type 2 Diabetes: A Randomized Double-Blind Placebo-Controlled Clinical Trial Homayouni F.et al,Phytother Res.,Aug 14
Salvianolic Acid A Protects Against Diabetic Nephropathy through Ameliorating Glomerular Endothelial Dysfunction via Inhibiting AGE-RAGE Signaling Hou B.et al,Cell Physiol Biochem,Dec 18
Oxidative DNA damage in the pathogenesis of chronic obstructive pulmonary disease in older patients O. Yu. Kytikova.et al,Adv Gerontol,Jul 22
Flavone protects HBE cells from DNA double-strand breaks caused by PM2. 5 Ren X.et al,Hum Cell,Nov 22
Role of oxidative stress and DNA hydroxymethylation in the neurotoxicity of fine particulate matter Wei H.et al,Toxicology. ,Jan 30
Evidence for Adaptive Response in a Molecular Epidemiological Study of the Inhabitants of a High Background-radiation Area of Yangjiang, China Su, Shibiao.et al,Health Physics,/
Photo-Oxidative Blue-Light Stimulation in Retinal Pigment Epithelium Cells Promotes Exosome Secretion and Increases the Activity of the NLRP3 Inflammasome Wei Zhang.et al,Current Eye Research,September 10
Serum 8-Hydroxy-2'-Deoxyguanosine Level as a Potential Biomarker of Oxidative DNA Damage Induced by Ionizing Radiation in Human Peripheral Blood Yu Gao, et al,Dose-Response,January 7
Changes of 8-OHdG and TrxR in the Residents Who Bathe in Radon Hot Springs Gao Yanxiao, et al,Dose-Response,January 7
Research Paper Effects of an 8-Week Aerobic Exercise Program on Some Indicators of Oxidative Stress in Elderly Women K Hejazi, et al,Iranian Journal Of Ageing,January 1
Evaluation of Serum 8-Hydroxy-Deoxyguanosine, Interleukin-6 and Interleukin-7 in Type 2 Diabetic Iraqi Females Alaaraji SFT, et al,Journal of global pharma technology,March 4

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Code	Product Name	Sample Types	Sensitivity
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Check Code：

Intra-assay Precision (Precision within an assay): CV%<6%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<11%

Three samples of known concentration were tested in twenty assays to assess.