Code | CSB-E10140h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
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Intra-assay Precision (Precision within an assay): CV%<12% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 84 | ||||
Range % | 80-91 | |||||
1:2 | Average % | 87 | ||||
Range % | 81-95 | |||||
1:4 | Average % | 88 | ||||
Range % | 82-95 | |||||
1:8 | Average % | 86 | ||||
Range % | 81-92 |
The recovery of human 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 89 | 84-96 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/ml | OD1 | OD2 | Average | Corrected | |||
800 | 0.337 | 0.344 | 0.340 | 0.340 | |||
200 | 0.873 | 0.857 | 0.865 | 0.865 | |||
50 | 1.302 | 1.328 | 1.315 | 1.315 | |||
12.5 | 1.784 | 1.699 | 1.741 | 1.741 | |||
3.12 | 2.190 | 2.291 | 2.240 | 2.240 | |||
0 | 2.534 | 2.453 | 2.493 | 2.493 |
This Human 8-OHdG ELISA Kit was designed for the quantitative measurement of Human 8-OHdG protein in serum, urine. It is a Competitive ELISA kit, its detection range is 3.12 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.
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