Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Name: Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit
Brand: CUSABIO
SKU: CSB-E10527m
Price: 150 USD
Availability: InStock
Rating: 5.0 (2 reviews)

Citations (24) Protocol

Code	CSB-E10527m
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (24)
Related Products
Customer Reviews and Q&A

Target Name

8-Hydroxy-desoxyguanosine,8-OHdG

Abbreviation

8-OHdG

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.78 pg/mL-50 pg/mL

Sensitivity

0.195 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
	Range %	87-97
1:200	Average %	97
	Range %	93-104
1:400	Average %	85
	Range %	82-91
1:800	Average %	90
	Range %	85-97

Recovery

The recovery of mouse 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	92	88-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
50	0.071	0.074	0.073
25	0.120	0.130	0.125
12.5	0.200	0.206	0.203
6.25	0.335	0.340	0.338
3.12	0.573	0.554	0.564
1.56	0.858	0.841	0.850
0.78	1.129	1.139	1.134
0	2.215	2.185	2.200

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse 8-OHdG ELISA Kit was designed for the quantitative measurement of Mouse 8-OHdG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.78 pg/mL-50 pg/mL and the sensitivity is 0.195 pg/mL.

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The Protective Effects of Melatonin Against Oxidative Stress and Inflammation Induced by Acute Cadmium Exposure in Mice Testis Li R. et al,Biol Trace Elem Res,2015

■ Customer Reviews

Average Rating:

5.0 - 2 reviews

Submit a Review here

Sample type: Serum

Sample species: Mouse

Sample dilution: : 1:200

Review: I used CSB-E10527m to detect the 8-OHdg level in the serum of mice in the normal group and the exposure group. In this experiment, the OD values of my standard curve were 1.07875, 1.02875, 0.91225, 0.6691, 0.4689, 0.21825, 0.08265, and 0.02685; the corresponding concentrations were 0, 0.78, 1.56, 3.12, 6.25, 12.5, 25, and 50 pg/ml. Sample OD value range: the original OD value was 0.1023 - 0.2823; the calculated concentration (pg/ml) was 10.17788 - 34.28991

By Anonymous

Sample type: Serum Tissue homogenate Ovary

Sample species: Mouse

Sample dilution: : 1:10,1:20,1:50,1:100,1:200

Review: I use CSB-E10527m to detect the 8-OHdg levels in mouse serum with different dilution concentrations and mouse ovaries of different weights; the mice are divided into control group, low/medium/high dose exposure groups.

By Anonymous

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.