Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Code CSB-E10527m
Size 96T,5×96T,10×96T
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Product Details

Target Name
8-Hydroxy-desoxyguanosine,8-OHdG
Abbreviation
8-OHdG
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 pg/mL-50 pg/mL
Sensitivity
0.195 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 90  
Range % 87-97  
1:200 Average % 97  
Range % 93-104  
1:400 Average % 85  
Range % 82-91  
1:800 Average % 90  
Range % 85-97  
Recovery
The recovery of mouse 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 92 88-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
50 0.071 0.074 0.073    
25 0.120 0.130 0.125    
12.5 0.200 0.206 0.203    
6.25 0.335 0.340 0.338    
3.12 0.573 0.554 0.564    
1.56 0.858 0.841 0.850    
0.78 1.129 1.139 1.134    
0 2.215 2.185 2.200    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse 8-OHdG ELISA Kit was designed for the quantitative measurement of Mouse 8-OHdG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.78 pg/mL-50 pg/mL and the sensitivity is 0.195 pg/mL.

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