| Description |
This Mouse 8-OHdG ELISA Kit was designed for the quantitative measurement of Mouse 8-OHdG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.78 pg/mL-50 pg/mL and the sensitivity is 0.195 pg/mL. |
| Target Name |
8-Hydroxy-desoxyguanosine,8-OHdG |
| Abbreviation |
8-OHdG |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.78 pg/mL-50 pg/mL |
| Sensitivity |
0.195 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology
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| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
90 |
|
| Range % |
87-97 |
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| 1:200 |
Average % |
97 |
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| Range % |
93-104 |
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| 1:400 |
Average % |
85 |
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| Range % |
82-91 |
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| 1:800 |
Average % |
90 |
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| Range % |
85-97 |
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| Recovery |
| The recovery of mouse 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
85-93 |
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| EDTA plasma (n=4) |
92 |
88-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml |
OD1 |
OD2 |
Average |
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| 50 |
0.071 |
0.074 |
0.073 |
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| 25 |
0.120 |
0.130 |
0.125 |
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| 12.5 |
0.200 |
0.206 |
0.203 |
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| 6.25 |
0.335 |
0.340 |
0.338 |
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| 3.12 |
0.573 |
0.554 |
0.564 |
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| 1.56 |
0.858 |
0.841 |
0.850 |
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| 0.78 |
1.129 |
1.139 |
1.134 |
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| 0 |
2.215 |
2.185 |
2.200 |
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
