Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

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Customer Reviews and Q&A

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Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Citations (35) Protocol MSDS

Code	CSB-E10526r
Size	96T,5×96T,10×96T
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Product Details
Citations (35)
Related Products
Customer Reviews and Q&A

Target Name

8-Hydroxy-desoxyguanosine,8-OHdG

Abbreviation

8-OHdG

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-98
1:2	Average %	96
	Range %	91-102
1:4	Average %	90
	Range %	85-96
1:8	Average %	96
	Range %	88-99

Recovery

The recovery of rat 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-107
EDTA plasma (n=4)	96	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.890	1.780	1.835	1.682
10	1.198	1.128	1.163	1.010
5	0.756	0.726	0.741	0.588
2.5	0.525	0.495	0.510	0.357
1.25	0.321	0.318	0.320	0.167
0.625	0.272	0.262	0.267	0.114
0.312	0.213	0.209	0.211	0.058
0	0.154	0.152	0.153

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat 8-OHdG ELISA Kit was designed for the quantitative measurement of Rat 8-OHdG protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Metabolomics analyses and comparative insight to neuroprotective potential of unripe fruits and leaves of Citrus aurantium ethanolic extracts against cadmium-induced rat brain dysfunction: involvement of oxidative stress and akt-mediated CREB/BDNF and GSK DAH Deabes, EAW El-Abd, SM Baraka,Metabolic Brain Disease,2025
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β-Sitosterol attenuates liver injury in a rat model of chronic alcohol intake Z Chen,Archives of Pharmacal Research,2020
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.