Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

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Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Name: Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit
Brand: CUSABIO
SKU: CSB-E10526r

^Instructions

Code	CSB-E10526r
Size	96T,5×96T,10×96T
Trial Size
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Target Name

8-Hydroxy-desoxyguanosine,8-OHdG

Abbreviation

8-OHdG

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-98
1:2	Average %	96
	Range %	91-102
1:4	Average %	90
	Range %	85-96
1:8	Average %	96
	Range %	88-99

Recovery

The recovery of rat 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-107
EDTA plasma (n=4)	96	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.890	1.780	1.835	1.682
10	1.198	1.128	1.163	1.010
5	0.756	0.726	0.741	0.588
2.5	0.525	0.495	0.510	0.357
1.25	0.321	0.318	0.320	0.167
0.625	0.272	0.262	0.267	0.114
0.312	0.213	0.209	0.211	0.058
0	0.154	0.152	0.153

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

β-Sitosterol attenuates liver injury in a rat model of chronic alcohol intake Z Chen,Archives of Pharmacal Research,2020
Antiapoptotic and antioxidant effects of resistance training with berberine consumption on diazinon induced cardiotoxicity in rats R Niazi,Journal of Medicinal Plants,2020
Protective Effects of Hyperbaric Oxygen Therapy on Brain Injury by Regulating the Phosphorylation of Drp1 Through ROS/PKC Pathway in Heatstroke Rats Ni XX,Cell Mol Neurobiol,2020
Resveratrol improves left ventricular remodeling in chronic kidney disease via Sirt1-mediated regulation of FoxO1 activity and MnSOD expression Li P, et al,Biofactors,2019
Isoorientin plays an important role in alleviating Cadmium-induced DNA damage and G0/G1 cell cycle arrest Shihao Chen, et al,Ecotoxicology and Environmental Safety,2019
Galangin Activates Nrf2 Signaling and Attenuates Oxidative Damage, Inflammation, and Apoptosis in a Rat Model of Cyclophosphamide-Induced Hepatotoxicity Aladaileh SH, et al,Biomolecules,2019
Amelioration of fenitrothion induced oxidative DNA damage and inactivation of caspase-3 in the brain and spleen tissues of male rats by N-acetylcysteine Rasha T. Alam,Life Sciences,2019
The beneficial effect of α-tocopherol succinate supplementation on DNA oxidation induced by intensive exercise training Hossein TaheriChadorneshin, et al,Sport Sciences for Health,2018
Aplysin Protects Against Alcohol‐Induced Liver Injury Via Alleviating Oxidative Damage and Modulating Endogenous Apoptosis‐Related Genes Expression in Rats Na Ge.et al,Journal of Food Science,2018
Puerarin Mitigates Diabetic Hepatic Steatosis and Fibrosis by inhibiting TGF-B signaling pathway activation in Type 2 Diabetic Rats Biyu Hou.et al,/,/
PM2.5 induces male reproductive toxicity via mitochondrial dysfunction, DNA damage and RIPK1 mediated apoptotic signaling pathway Jin Zhang.et al,Science of The Total Environment,2018
The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract in Renal Ischemia/Reperfusion Injury in a Streptozotocin-Induced Diabetic Model Gehan A Hegazy.et al,Biomedical and Pharmacology,2018
Dietary Selenium Deficiency or Excess Reduces Sperm Quality and Testicular mRNA Abundance of Nuclear Glutathione Peroxidase 4 in Rats Zhou JC.et al,J Nutr.,2017
Fucoidan Protects Dopaminergic Neurons by Enhancing the Mitochondrial Function in a Rotenone-induced Rat Model of Parkinson’s Disease Zhang Li.et al,Aging Dis,2017
Nandrolone administration with or without strenuous exercise promotes overexpression of nephrin and podocin genes and induces structural and functional Tofighi A.et al,Toxicol Lett,2017
Rescue effects of ginger extract on dose dependent radiation-induced histological and biochemical changes in the kidneys of male Wistar rats Saberi H.et al,Biomed Pharmacother.,2017
Inhibition of Rac1 ameliorates neuronal oxidative stress damage via reducing Bcl-2/Rac1 complex formation in mitochondria through PI3K/Akt/mTOR Pan Y.et al,Exp Neurol,2017
Restoration of Spermatogenesis Using a New Combined Herbal Formula of Epimedium koreanum Nakai and Angelica gigas Nakai in an Luteinizing HyunJun Park.et al,World J Mens Health,2017
Ginger extract mitigates ethanol-induced changes of alpha and beta-myosin heavy chain isoforms gene expression and oxidative stress in the heart of male wistar rats Alireza Shirpoor.et al,DNA Repair,2017
Effect of intensive endurance training on rat brain and hepatic 8-oxoguanine DNA glycosylase and 8-hydroxy-2'-deoxyguanosine levels Mohammad Afzalpour.et al,Journal of Applied Pharmaceutical Science ,2016
Metabonomics revealed xanthine oxidase-induced oxidative stress and inflammation in the pathogenesis of diabetic nephropathy Liu J.et al,Anal Bioanal Chem.,2015
Assessment of benzene induced oxidative impairment in rat isolated pancreatic islets and effect on insulin secretion Haji Bahadar et al,Environmental Toxicology and Pharmacology,2015
The effect of prenatal exposure to 900-megahertz electromagnetic field on the, 21-old-day rat testicle Hanc H et al,Reprod Toxicol,2013

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.