| Target Name |
8-Hydroxy-desoxyguanosine,8-OHdG |
| Abbreviation |
8-OHdG |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
95 |
|
| Range % |
88-98 |
|
| 1:2 |
Average % |
96 |
|
| Range % |
91-102 |
|
| 1:4 |
Average % |
90 |
|
| Range % |
85-96 |
|
| 1:8 |
Average % |
96 |
|
| Range % |
88-99 |
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| Recovery |
| The recovery of rat 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
102 |
98-107 |
|
| EDTA plasma (n=4) |
96 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
1.890 |
1.780 |
1.835 |
1.682 |
|
| 10 |
1.198 |
1.128 |
1.163 |
1.010 |
|
| 5 |
0.756 |
0.726 |
0.741 |
0.588 |
|
| 2.5 |
0.525 |
0.495 |
0.510 |
0.357 |
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| 1.25 |
0.321 |
0.318 |
0.320 |
0.167 |
|
| 0.625 |
0.272 |
0.262 |
0.267 |
0.114 |
|
| 0.312 |
0.213 |
0.209 |
0.211 |
0.058 |
|
| 0 |
0.154 |
0.152 |
0.153 |
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Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
