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Human Activin A,ACV-A ELISA Kit

Human Activin A,ACV-A ELISA Kit

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Citations (3) Protocol MSDS

Code	CSB-E04486h
Size	96T,5×96T,10×96T
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Product Details
Citations (3)
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Product Details

Target Name

Activin A,ACV-A

Abbreviation

ACV-A

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	125.907	124.505	129.620	124.680	126.082	125.731
SD	0.037	0.035	0.042	0.045	0.048	0.049
CV(%)	3.532	3.366	3.930	4.323	4.576	4.681

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

80-92

1:2

Average %

Range %

90-99

1:4

Average %

Range %

92-101

1:8

Average %

105

Range %

100-110

Recovery

The recovery of human ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	93	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

3.254

3.125

3.190

3.072

500

2.329

2.414

2.372

2.254

250

1.588

1.626

1.607

1.489

125

1.098

1.040

1.069

0.951

62.5

0.677

0.634

0.656

0.538

31.2

0.395

0.377

0.386

0.268

15.6

0.206

0.198

0.202

0.084

0.117

0.118

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human ACV-A ELISA Kit was designed for the quantitative measurement of Human ACV-A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Citations

H Antigen expression modulates epidermal Keratinocyte Integrity and differentiation SP Jin, JH Oh, NK Kim, JH Chung,Biological Research,2024
Effects of Icelandic yogurt consumption and resistance training in healthy untrained older males R Bagheri,British Journal of Nutrition,2021
Comparison of whole egg v. egg white ingestion during 12 weeks of resistance training on skeletal muscle regulatory markers in resistance-trained men R Bagheri,Br J Nutr,2020

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Human Activin A,ACV-A ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A