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Rat Activin A,ACV-A ELISA Kit

Rat Activin A,ACV-A ELISA Kit

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Citations (1) Protocol MSDS

Code	CSB-E04503r
Size	96T,5×96T,10×96T
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Citations (1)
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Product Details

Target Name

Activin A,ACV-A

Abbreviation

ACV-A

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	250.287	251.377	254.271	255.277	253.524	253.271
SD	0.029	0.030	0.034	0.044	0.042	0.045
CV(%)	3.76	4.61	4.83	6.52	6.46	7.08

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

87-93

1:2

Average %

101

Range %

96-108

1:4

Average %

Range %

84-91

1:8

Average %

100

Range %

95-105

Recovery

The recovery of rat ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	87	83-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.582

2.601

2.592

2.474

500

1.959

1.869

1.914

1.796

250

1.301

1.298

1.300

1.182

125

0.823

0.831

0.827

0.709

62.5

0.498

0.501

0.500

0.382

31.2

0.325

0.336

0.331

0.213

15.6

0.237

0.241

0.239

0.121

0.117

0.119

0.118

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat ACV-A ELISA Kit was designed for the quantitative measurement of Rat ACV-A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Citations

Colchicine: Emerging therapeutic effects on atrial fibrillation by alleviating myocardial fibrosis in a rat model H Yue,Biomedicine & pharmacotherapy,2022

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Rat Activin A,ACV-A ELISA Kit

Product Details

Citations

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