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Mouse Activin A,ACV-A ELISA Kit

Mouse Activin A,ACV-A ELISA Kit

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Protocol MSDS

Code	CSB-E04502m
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details

Target Name

Activin A,ACV-A

Abbreviation

ACV-A

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	122.000	132.198	122.651	123.043	120.448	120.448
SD	0.055	0.064	0.063	0.071	0.068	0.074
CV(%)	5.56	6.01	6.34	7.12	6.96	7.58

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

86-105

1:2

Average %

Range %

91-100

1:4

Average %

Range %

90-99

1:8

Average %

Range %

89-100

Recovery

The recovery of mouse ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	97	92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.249

2.268

2.259

2.175

500

2.035

2.055

2.045

1.961

250

1.601

1.586

1.594

1.510

125

0.954

0.972

0.963

0.879

62.5

0.534

0.521

0.528

0.444

31.2

0.272

0.283

0.278

0.194

15.6

0.184

0.190

0.187

0.103

0.083

0.085

0.084

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse ACV-A ELISA Kit was designed for the quantitative measurement of Mouse ACV-A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Mouse Activin A,ACV-A ELISA Kit

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