Human B-cell differentiation antigen CD72(CD72) ELISA kit

Code CSB-EL004955HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human CD72 ELISA Kit was designed for the quantitative measurement of Human CD72 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.225 ng/mL.

Target Name CD72 molecule
Alternative Names CD72; B-cell differentiation antigen CD72; Lyb-2; CD antigen CD72
Abbreviation CD72
Uniprot No. P21854
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.9 ng/mL-60 ng/mL
Sensitivity 0.225 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 92-103
1:2 Average % 91
Range % 86-98
1:4 Average % 92
Range % 88-97
1:8 Average % 101
Range % 96-15
Recovery
The recovery of human CD72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 87-95
EDTA plasma (n=4) 101 103-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
60 2.588 2.787 2.688 2.535
30 2.169 2.236 2.203 2.050
15 1.674 1.744 1.709 1.556
7.5 1.322 1.223 1.273 1.120
3.75 0.752 0.744 0.748 0.595
1.8 0.363 0.375 0.369 0.216
0.9 0.261 0.255 0.258 0.105
0 0.149 0.156 0.153  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Plays a role in B-cell proliferation and differentiation.
Gene References into Functions
  1. Interferon-alpha-induced CD100 expression on naive CD8(+) T cells enhances antiviral responses to hepatitis C infection through CD72 signal transduction. PMID: 28222623
  2. Increased soluble CD72 in systemic lupus erythematosus is in association with disease activity and lupus nephritis PMID: 26883681
  3. CD72 expression on activated B cells of SLE patients was significantly lower than that of normal controls. sema3A enhanced CD72 expression of B cells, but it was still lower in SLE patients than in normal individuals. PMID: 24461079
  4. data demonstrated aberrant expression of CD72 exists on B cells of myasthenia gravis and multiple sclerosis patients and expression level of CD72 molecule has a significantly negative correlation with anti-AchR antibody levels in myasthenia gravis PMID: 23184497
  5. regulates serum immunoglobulin leveln and disease resistance in systemic lupus erythematosus PMID: 23268649
  6. CD72 mRNA expression level correlates with Sema4D expression in peripheral blood mononuclear cells in immune thrombocytopenia. PMID: 22111667
  7. CD100, CD72 and CD45 were expressed in placenta and exhibited different mRNA and protein levels in normal pregnancy and miscarriage. Protein levels were highly dysregulated around 10 weeks of gestation in first and second miscarriage placentas. PMID: 22606231
  8. CD100-CD72 interaction can be the mechanism by which NK cell communicate with B cells. PMID: 17786190
  9. Data show that ligation of CD72 with the BU40, or with rCD100 negatively regulates KIT-mediated mast cell proliferation, chemotaxis, and chemokine production. PMID: 20100931
  10. results indicated that the presence of CD72-*2 allele decreases risk for human systemic lupus erythematosus conferred by FCGR2B-232Thr, possibly by increasing the AS isoform of CD72 PMID: 15459183
  11. CD72 is a key molecule in regulating mature B cell differentiation; CD72 signaling reduces the expression of X-box binding protein 1 in B cells PMID: 16047337
  12. increased nucleotide mutation of CD72 mRNA accounts for the decreased expression level of CD72 in B cells, and it might be related to hyperactivity of B cells in patients with SLE PMID: 17121583
  13. CD72 polymorphisms are associated with age of appearing clinical manifestations in idiopathic thrombocytopenic purpura in Chinese patients. PMID: 18071878

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Subcellular Location Membrane; Single-pass type II membrane protein.
Tissue Specificity Pre-B-cells and B-cells but not terminally differentiated plasma cells.
Database Links

HGNC: 1696

OMIM: 107272

KEGG: hsa:971

STRING: 9606.ENSP00000259633

UniGene: Hs.116481

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