Product Details

Description

This Human CD72 ELISA Kit was designed for the quantitative measurement of Human CD72 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.225 ng/mL.

Target Name

CD72 molecule

Alternative Names

CD72; B-cell differentiation antigen CD72; Lyb-2; CD antigen CD72

Abbreviation

CD72

Uniprot No.

P21854

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.9 ng/mL-60 ng/mL

Sensitivity

0.225 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-103
1:2	Average %	91
	Range %	86-98
1:4	Average %	92
	Range %	88-97
1:8	Average %	101
	Range %	96-15

Recovery

The recovery of human CD72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	101	103-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.588	2.787	2.688	2.535
30	2.169	2.236	2.203	2.050
15	1.674	1.744	1.709	1.556
7.5	1.322	1.223	1.273	1.120
3.75	0.752	0.744	0.748	0.595
1.8	0.363	0.375	0.369	0.216
0.9	0.261	0.255	0.258	0.105
0	0.149	0.156	0.153

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Plays a role in B-cell proliferation and differentiation.
Gene References into Functions	Interferon-alpha-induced CD100 expression on naive CD8(+) T cells enhances antiviral responses to hepatitis C infection through CD72 signal transduction. PMID: 28222623 Increased soluble CD72 in systemic lupus erythematosus is in association with disease activity and lupus nephritis PMID: 26883681 CD72 expression on activated B cells of SLE patients was significantly lower than that of normal controls. sema3A enhanced CD72 expression of B cells, but it was still lower in SLE patients than in normal individuals. PMID: 24461079 data demonstrated aberrant expression of CD72 exists on B cells of myasthenia gravis and multiple sclerosis patients and expression level of CD72 molecule has a significantly negative correlation with anti-AchR antibody levels in myasthenia gravis PMID: 23184497 regulates serum immunoglobulin leveln and disease resistance in systemic lupus erythematosus PMID: 23268649 CD72 mRNA expression level correlates with Sema4D expression in peripheral blood mononuclear cells in immune thrombocytopenia. PMID: 22111667 CD100, CD72 and CD45 were expressed in placenta and exhibited different mRNA and protein levels in normal pregnancy and miscarriage. Protein levels were highly dysregulated around 10 weeks of gestation in first and second miscarriage placentas. PMID: 22606231 CD100-CD72 interaction can be the mechanism by which NK cell communicate with B cells. PMID: 17786190 Data show that ligation of CD72 with the BU40, or with rCD100 negatively regulates KIT-mediated mast cell proliferation, chemotaxis, and chemokine production. PMID: 20100931 results indicated that the presence of CD72-*2 allele decreases risk for human systemic lupus erythematosus conferred by FCGR2B-232Thr, possibly by increasing the AS isoform of CD72 PMID: 15459183 CD72 is a key molecule in regulating mature B cell differentiation; CD72 signaling reduces the expression of X-box binding protein 1 in B cells PMID: 16047337 increased nucleotide mutation of CD72 mRNA accounts for the decreased expression level of CD72 in B cells, and it might be related to hyperactivity of B cells in patients with SLE PMID: 17121583 CD72 polymorphisms are associated with age of appearing clinical manifestations in idiopathic thrombocytopenic purpura in Chinese patients. PMID: 18071878 Show More Hide All
Subcellular Location	Membrane; Single-pass type II membrane protein.
Tissue Specificity	Pre-B-cells and B-cells but not terminally differentiated plasma cells.
Database Links	HGNC: 1696 OMIM: 107272 KEGG: hsa:971 STRING: 9606.ENSP00000259633 UniGene: Hs.116481

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Code	CSB-EL004955HU
Size	96T,5×96T,10×96T
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Human B-cell differentiation antigen CD72(CD72) ELISA kit

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