Human C-type lectin domain family 18 member A(CLEC18A) ELISA kit

Code CSB-EL005521HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human CLEC18A ELISA Kit was designed for the quantitative measurement of Human CLEC18A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name C-type lectin domain family 18, member A
Alternative Names C-type lectin domain family 18 member A ELISA Kit; CL18A_HUMAN ELISA Kit; CLEC18A ELISA Kit; FLJ77955 ELISA Kit; Mannose receptor-like protein 2 ELISA Kit; MGC164804 ELISA Kit; Mrcl ELISA Kit; MRCL1 ELISA Kit; MRLP2 ELISA Kit
Abbreviation CLEC18A
Uniprot No. A5D8T8
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC18A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-97  
1:2 Average % 99  
Range % 95-107  
1:4 Average % 89  
Range % 85-93  
1:8 Average % 84  
Range % 80-88  
Recovery
The recovery of human CLEC18A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 95-107  
EDTA plasma (n=4) 90 85-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.439 2.550 2.495 2.362  
10 1.825 1.956 1.891 1.758  
5 1.273 1.224 1.249 1.116  
2.5 0.671 0.693 0.682 0.549  
1.25 0.457 0.427 0.442 0.309  
0.625 0.309 0.317 0.313 0.180  
0.312 0.242 0.239 0.241 0.108  
0 0.130 0.135 0.133    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Binds polysaccharides in a Ca(2+)-independent manner with a preferentially binding to fucoidan, beta-glucans and galactans.
Gene References into Functions
  1. Plasma CLEC18 levels were correlated with the stage of HBV infection and could predict HBeAg loss and seroconversion in the patients with chronic hepatitis B (CHB) undergoing NUC therapy PMID: 30055605
Subcellular Location Secreted. Endoplasmic reticulum. Golgi apparatus. Endosome.
Tissue Specificity Dectected in all cell lines tested and in peripheral blood cells.
Database Links

HGNC: 30388

OMIM: 616571

KEGG: hsa:348174

STRING: 9606.ENSP00000288040

UniGene: Hs.592064

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