Human Collagen, type III, alpha 1 (COL3A1)ELISA Kit

Code CSB-E13446h
Size 96T,5×96T,10×96T
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Product Details

Target Name
collagen, type III, alpha 1
Alternative Names
Alpha 1 type III collagen ELISA Kit; Alpha1 (III) collagen ELISA Kit; CO3A1_HUMAN ELISA Kit; COL 3A1 ELISA Kit; COL3A1 ELISA Kit; Collagen alpha 1(III) chain ELISA Kit; Collagen alpha-1(III) chain ELISA Kit; Collagen III alpha 1 chain precursor ELISA Kit; Collagen III alpha 1 polypeptide ELISA Kit; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) ELISA Kit; Collagen type III alpha 1 ELISA Kit; Collagen type III alpha 1 chain ELISA Kit; Collagen type III alpha ELISA Kit; Collagen; fetal ELISA Kit; EDS4A ELISA Kit; Ehlers Danlos syndrome type IV; autosomal dominant ELISA Kit; Fetal collagen ELISA Kit; Type III collagen ELISA Kit
Abbreviation
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human COL3A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 85-96  
1:2 Average % 100  
Range % 95-106  
1:4 Average % 101  
Range % 95-106  
1:8 Average % 96  
Range % 90-107  
Recovery
The recovery of human COL3A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 85-98  
EDTA plasma (n=4) 109 102-115  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.484 2.575 2.530 2.381  
5 1.788 1.889 1.839 1.690  
2.5 1.256 1.365 1.311 1.162  
1.25 0.732 0.766 0.749 0.600  
0.625 0.417 0.450 0.434 0.285  
0.312 0.274 0.284 0.279 0.130  
0.156 0.248 0.250 0.249 0.100  
0 0.146 0.151 0.149    
             
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human COL3A1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled COL3A1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human COL3A1 ELISA Kit was designed for the quantitative measurement of Human COL3A1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

Function
(From Uniprot)
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Gene References into Functions
  1. Elevated circulating PIIINP levels are associated with type 2 diabetes mellitus individuals with adipose tissue expansion and systemic proinflammatory profile suggestive for adipose tissue dysfunction. PMID: 29471595
  2. Reduced expression of types I and III collagen and TIMP-1 as well as the increased expression of MMP-1 and MMP-8 in the anterior vaginal wall tissues play important roles in the onset of pelvic organ prolapse. PMID: 29263043
  3. a variant of COL3A1 (rs3134646) is associated with the risk of developing colonic diverticulosis in white men, whereas rs1800255 (COL3A1) and rs1800012 (COL1A1) were not associated with this condition after adjusting for confounding factors. PMID: 29533249
  4. Brain MRI in the affected siblings as well as in the two previously reported individuals with bi-allelic COL3A1 mutations showed a brain phenotype similar to that associated with mutations in GPR56. PMID: 28258187
  5. Given the high specificity of the polymorphism at the rs1800255 locus of the COL3A1 gene, determined by the Sanger sequencing, it can be concluded that there is an association between this polymorphism and urinary incontinence and pelvic organ prolapse in women. PMID: 29376591
  6. Case Report: novel missense COL3A1 mutation in vascular Ehlers-Danlos syndrome patient presenting with pulmonary complications and iliac arterial dissection. PMID: 29216800
  7. Kaplan-Meir analysis of GSE7696 indicate that COL3A1 and SNAP91 correlated with survival. PMID: 27655637
  8. provide a picture of the gene expression changes in vascular Ehlers-Danlos syndrome skin fibroblasts and highlight that dominant negative mutations in COL3A1 also affect post-translational modifications and deposition into the ECM of several structural proteins crucial to the integrity of soft connective tissues PMID: 29346445
  9. We identified biallelic COL3A1 variants in two unrelated families. In a 3-year-old female with developmental delay the nonsense variant c.1282C>T, p.(Arg428*) was detected in combination the c.2057delC, p.(Pro686Leufs*105) frame shift variant. PMID: 28742248
  10. In conclusion, based on serological collagen formation and degradation makers, penetrating Crohn's disease is associated with increased matrix metalloproteinase-9 mediated breakdown of type III collagen PMID: 28481042
  11. Col3A may be a potential adjunct marker for both differentiating fibroadenoma from phyllodes tumor and assessing malignant potential in PTs. PMID: 27498063
  12. Case Report: pathogenetic heterozygous COL3A1 mutation c.3140 G>A, p. Gly1047Asp in Ehlers-Danlos syndrome vascular type with different phenotypes in the same family. PMID: 28183226
  13. von Willebrand factor A1 domain-collagen binding is independent of gain- or loss-of-function phenotype and under shear stress, platelet translocation pause times on collagen-bound A1A2A3 are either normal or shorter depending on whether A1 is concertedly bound with the A3 domain to collagen. PMID: 27889474
  14. High serum procollagen type III N-terminal peptide expression is associated with non-alcoholic fatty liver disease. PMID: 27442361
  15. The findings indicate the critical role of CatB in regulating the expression of collagens III and IV by fibroblasts via prolonging TLR2/NF-kappaB activation and oxidative stress. PMID: 27648120
  16. High COL3A1 expression is associated with colorectal carcinoma. PMID: 26741506
  17. novel missense mutation c.2176G>C in Chinese family with vascular Ehlers-Danlos syndrome PMID: 25420629
  18. High serum Collagen Type III is associated with ovarian and breast cancer. PMID: 26406420
  19. A novel missense mutation in COL3A1 was found in a young patient with cervical artery dissection as the single manifestation of Ehlers-Danlos syndrome. PMID: 26497932
  20. the clinical phenotype of Ehlers-Danlos syndrome patients is influenced by the type of COL3A1 variant. PMID: 25758994
  21. Report dysregulated expression of COL3A1 in disc degeneration. PMID: 25893343
  22. TGFbeta target genes including TGFBI, BAMBI, COL3A1 and SERPINE1 are significantly increased in Diamond Blackfan Anemia induced pluripotent stem cells PMID: 26258650
  23. The production and purification of rhCOL3A1 described in this study offer a new method for obtaining high level of rhCOL3A1 in relatively pure form, which is suitable for biomedical materials application. PMID: 25231012
  24. These results suggest that let-7d may suppress renal cell carcinoma growth, metastasis, and tumor macrophage infiltration at least partially through targeting COL3A1 and CCL7. PMID: 25193015
  25. compare reported phenotypes for patients with missense variants in the C-propeptide domain for other human collagen disorders including COL1A1 and COL1A2 (osteogenesis imperfecta). PMID: 25846194
  26. miR-29a and miR-29b enhance cell migration and invasion in nasopharyngeal carcinoma progression by regulating SPARC and COL3A1 gene expression. PMID: 25786138
  27. Data indicate that N-terminal propeptide of type III procollagen (PIIINP) is a highly effective means to evaluate left ventricular (LV) end-diastolic pressure (EDP) in patients with acute coronary syndrome (ACS). PMID: 25559610
  28. The first main finding of this study was that the rare COL3A1 rs1800255 AA genotype was associated with increased risk of anterior cruciate ligament injuries in the Polish cohort but not the South African cohort. PMID: 25073002
  29. During heart valve development, Krox20-mediated activation of fibrillar Col1a1 and Col3a1 genes is crucial to avoid postnatal degeneration of the aortic valve leaflets. PMID: 25344368
  30. In familial AAA we found one pathogenic and segregating variant (COL3A1 p.Arg491X), one likely pathogenic and segregating (MYH11 p.Arg254Cys), and fifteen VUS. PMID: 26017485
  31. rs1800255, COL3A1 2209 G>A polymorphism not associated with pelvic organ prolapse in Dutch population PMID: 24760181
  32. Variants of the COL3A1 gene are associated with a risk of stroke recurrence and prognosis. PMID: 24664438
  33. As a result of the translocations, COL3A1-PLAG1, the constitutively active promoter of the partner gene drives the ectopic expression of PLAG1. PMID: 24700772
  34. Gal-1 decreased the expression of collagen genes COL3A1 and COL5A1 but increased the expression of fibronectin and laminin 5. PMID: 24503541
  35. Arterial pathology in vascular Ehlers-Danlos syndrome individuals is related to the underlying COL3A1 mutation type. PMID: 24650746
  36. Expression of COL3A1 mRNA encoding key fibrotic extracellular matrix molecules was down-regulated by pre-miRNA-29b. PMID: 24641356
  37. The results point to the fact that the injury and/or mandible fracture increase the collagen type III metabolism and its dynamics depends on the type of the used bone fixation. PMID: 24421219
  38. Case Report: Ehlers-Danlos syndrome IV due to a mutation in intron 14 of the COL3A1 gene leading to venous manifestations without affecting arterial vessels at clinical presentation. PMID: 23688910
  39. Data suggest that cardiac fibrosis, as assessed by serum extracellular matrix protein biomarkers (including PIIINP, procollagen Type III-N-terminal peptide), develops early in hypertensive patients and is predictive of cardiovascular events or death. PMID: 22788708
  40. analysis of collagen folding in Vascular Ehlers-Danlos syndrome mutations PMID: 23645670
  41. COL3A1 rs1800255, COL6A1 rs35796750 and COL12A1 rs970547 were not significantly associated with sit-and-reach, straight leg raise or total shoulder rotation range of motion PMID: 23013106
  42. This study indicates that SP, mediated via NK-1 R, increases collagen remodeling and leads to increased MMP3 mRNA and protein expression that is further enhanced by cyclic mechanical loading. PMID: 22836729
  43. IL-17, IL-23 and PIIINP have an inverse correlation with vitamin D in their involvement in the immune response in patients with HCV-4-related liver diseases in Egypt PMID: 22851868
  44. study found that allele A of SNP rs1800255 conferred a 1.71-fold increased risk for intracranial aneurysms (IAs) and results in an amino acid change of Ala698Thr, which led to a lower thermal stability of the peptide; results support the view that the functional variant of COL3A1 is genetic risk factors for IAs in the Chinese population PMID: 22241462
  45. The minimum type III sequence necessary for cleavage by the MMP1 and MMP13 was 5 GXY triplets, including 4 residues before and 11 residues after the cleavage site (P4-P11'). PMID: 22573319
  46. Alveolar macrophages exposed to SiO2 can induce elevated type III procollagen and collagen III expression levels in human lung fibroblasts. PMID: 21941774
  47. Through alveolar macrophage mediation SiO2 can accelerate the expression of TIMP-1 and collagen III, and inhibit the expression of MMP-1 in human lung fibroblasts. PMID: 20039537
  48. procollagen III N-terminal peptide has a role in HBeAg loss in patients with chronic hepatitis B during entecavir therapy PMID: 22019950
  49. Disease-associated mutations prevent GPR56-collagen III interaction. PMID: 22238662
  50. Data show that homozygous and compound heterozygous changes found in PLOD1 and SLC2A10 may confer autosomal recessive effects, and three MYH11, ACTA2 and COL3A1 heterozygous variants were considered as putative pathogenic gene alterations. PMID: 22001912

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Involvement in disease
Ehlers-Danlos syndrome 3 (EDS3); Ehlers-Danlos syndrome 4 (EDS4)
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Fibrillar collagen family
Database Links

HGNC: 2201

OMIM: 120180

KEGG: hsa:1281

STRING: 9606.ENSP00000304408

UniGene: Hs.443625

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