Human Collectin-10(COLEC10) ELISA kit

Code CSB-EL005760HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human COLEC10 ELISA Kit was designed for the quantitative measurement of Human COLEC10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

Target Name collectin sub-family member 10 (C-type lectin)
Alternative Names CL-34 ELISA Kit; CL-L1 ELISA Kit; CLL1 ELISA Kit; COL10_HUMAN ELISA Kit; Colec10 ELISA Kit; Collectin liver 1 ELISA Kit; Collectin liver protein 1 ELISA Kit; Collectin sub family member 10 (C-type lectin) ELISA Kit; Collectin sub family member 10 ELISA Kit; Collectin subfamily member 10 ELISA Kit; Collectin-10 ELISA Kit; Collectin-34 ELISA Kit; MGC118794 ELISA Kit; MGC118795 ELISA Kit; OTTHUMP00000228058 ELISA Kit
Abbreviation COLEC10
Uniprot No. Q9Y6Z7
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %81-92
1:2Average %92
Range %83-98
1:4Average %97
Range %93-101
1:8Average %95
Range %87-99
Recovery
The recovery of human COLEC10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-102
EDTA plasma (n=4)8882-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.880 2.964 2.922 2.814
7502.541 2.427 2.484 2.376
3751.832 1.734 1.783 1.675
187.50.997 0.972 0.985 0.877
93.750.533 0.554 0.544 0.436
46.880.380 0.376 0.378 0.270
23.440.254 0.258 0.256 0.148
00.107 0.108 0.108
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Lectin that binds to various sugars: galactose > mannose = fucose > N-acetylglucosamine > N-acetylgalactosamine. Acts as a chemoattractant, probably involved in the regulation of cell migration.
Gene References into Functions
  1. High CLL1 expression is associated with cardiovascular disease. PMID: 27341702
  2. Exome sequencing reveals mutations in collectin sub-family member 10 (C-type lectin) protein (COLEC10) as a cause of 3MC syndrome with main features include facial dysmorphism, craniosynostosis and cleft lip/palate. PMID: 28301481
  3. high serum CL-L1 concentration in critically ill children upon PICU admission is associated with an increased risk of infection and prolonged need of intensive care, and counteracts the protective effect of having a high MASP-3 concentration PMID: 27057739
  4. Data indicate differences in the plasma concentrations of collectin liver 1 and collectin kidney 1, M-ficolin and H-ficol in systemic lupus erythematosus (SLE) patients compared to a group of healthy controls. PMID: 26154564
  5. Carriers of COLEC10 Arg125Trp had increased CL-L1 serum levels. PMID: 25710878
  6. Most plasma CL-K1 was found in complex with CL-L1 in a ratio suggesting a heteromeric subunit of 1 CL-L1 & 2 CL-K1 polypeptides. PMID: 24174618

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Involvement in disease 3MC syndrome 3 (3MC3)
Subcellular Location Secreted. Golgi apparatus. Cytoplasm.
Protein Families COLEC10/COLEC11 family
Tissue Specificity Highly expressed in liver, placenta and adrenal gland. Moderately expressed in small intestine, lung, stomach and prostate. Weakly expressed in trachea and spleen.
Database Links

HGNC: 2220

OMIM: 248340

KEGG: hsa:10584

STRING: 9606.ENSP00000332723

UniGene: Hs.176615

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