Product Details

Target Name

C1q and tumor necrosis factor related protein 1

Alternative Names

C1q and tumor necrosis factor related protein 1 ELISA Kit; C1QT1_HUMAN ELISA Kit; C1QTNF1 ELISA Kit; Complement C1q tumor necrosis factor related protein 1 ELISA Kit; Complement C1q tumor necrosis factor-related protein 1 ELISA Kit; CTRP 1 ELISA Kit; CTRP1 ELISA Kit; FLJ90694 ELISA Kit; G protein coupled receptor interacting protein 1 ELISA Kit; G protein-coupled receptor-interacting protein ELISA Kit; GIP ELISA Kit; ZSIG37 ELISA Kit

Abbreviation

C1QTNF1

Uniprot No.

Q9BXJ1

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CTRP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	87-95
1:8	Average %	104
	Range %	97-108

Recovery

The recovery of human CTRP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	103	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.274	2.312	2.293	2.190
10	1.258	1.354	1.306	1.203
5	0.698	0.735	0.717	0.614
2.5	0.430	0.447	0.439	0.336
1.25	0.275	0.288	0.282	0.179
0.625	0.174	0.184	0.179	0.076
0.312	0.139	0.147	0.143	0.040
0	0.102	0.103	0.103

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human C1QTNF1 ELISA Kit was designed for the quantitative measurement of Human C1QTNF1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Background

Gene References into Functions

CTRP1 has a role in linking dysregulation of lipid metabolism and inflammatory responses in macrophages PMID: 27175610
High serum CTRP1 level is associated with Type 2 diabetes mellitus. PMID: 28207876
In cultured endothelial cells, treatment of CTRP1 led to increased permeability to fluorescent-labelled dextran and apparent formation of paracellular holes as observed after disturbed flow exposure, which was evidently reduced in the presence of a CTRP1-specific neutralizing antibody. PMID: 28625919
Increased serum CTRP 1 levels were closely associated with the prevalence and severity of CAD, it might be regarded as a marker for myocardial infarction. PMID: 27169633
the strong association of CTRP1 with insulin resistance in NAFLD PMID: 25767880
The levels of circulating CTRP1 are associated with coronary artery disease in men. PMID: 24945145
The variation of plasma CTRP-1 and IL-6 concentrations may play an important role in reflecting the degree of inflammation in CHD and the severity of coronary arterial atherosclerosis. PMID: 25635749
Increased plasma CTRP1 was independently associated with type 2 diabetes. Profiling of plasma adipokines such as CTRP1 is particularly important to obtain a greater understanding of their contribution to the type 2 diabetic state. PMID: 24965225
CTRP1 is a novel adipokine associated with type 2 diabetes mellitus in humans. PMID: 24827430
CTRP1 level was significantly higher in subjects with metabolic syndrome compared to healthy subjects. PMID: 23000311
The review describes the potential role of C1qTNF1 as an intracellular regulator of signal transduction PMID: 16386877
molecular cloning, gene expression, and subcellular localization of GIP PMID: 12409230
These findings indicate that CTRP1 expression may be associated with a low-grade chronic inflammation status in adipose tissues. PMID: 16806199
CTRP1 enhances the production of aldosterone. CTRP1 was highly expressed in obese subjects as well as up-regulated in hypertensive patients, CTRP1 may be a newly identified molecular link between obesity and hypertension. PMID: 18171693
CTRP1 may be considered a novel adipokine, providing an important framework to further address the physiological functions and mechanisms of the action of this family of secreted glycoproteins in normal and disease states. PMID: 18783346

Hide All

Subcellular Location

Secreted.

Database Links

HGNC: 14324

OMIM: 610365

KEGG: hsa:114897

STRING: 9606.ENSP00000340864

UniGene: Hs.201398

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-E16713h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human Complement C1q tumor necrosis factor-related protein 1(C1QTNF1/CTRP1) ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background