Human Complement component C6(C6) ELISA kit

Code CSB-EL004036HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human complement component 6 ELISA Kit was designed for the quantitative measurement of Human complement component 6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Target Name complement component 6
Alternative Names AW111623 ELISA Kit; C6 ELISA Kit; CO6_HUMAN ELISA Kit; Complement component 6 ELISA Kit; Complement component C6 ELISA Kit
Abbreviation C6
Uniprot No. P13671
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:10000 Average % 88
Range % 81-92
1:20000 Average % 95
Range % 91-105
1:40000 Average % 97
Range % 92-101
1:80000 Average % 93
Range % 86-98
Recovery
The recovery of human C6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 85-98
EDTA plasma (n=4) 95 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 2.021 2.107 2.064 1.940
2000 1.745 1.784 1.765 1.641
1000 1.318 1.329 1.324 1.200
500 0.902 0.894 0.898 0.774
250 0.651 0.668 0.660 0.536
125 0.489 0.492 0.491 0.367
62.5 0.337 0.341 0.339 0.215
0 0.122 0.125 0.124  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
Gene References into Functions
  1. Our study provided novel evidence that genetic variations in complement genes C6 and MASP1were associated with preeclampsia risk, and that the risk varied by preeclampsia subtypes PMID: 27405496
  2. recipient C6 rs9200 polymorphism is associated with HCC recurrence after OLT, and improves the predictive value of clinical models. PMID: 27173880
  3. Structure of complement C6 suggests a mechanism for initiation and unidirectional, sequential assembly of membrane attack complex (MAC). PMID: 22267737
  4. finding of very high serious morbidity in patients with complete C6 deficiency due to meningococcal infections PMID: 22288589
  5. Genetic polymorphisms in C6 gene do not influence the risk of aspirin hypersensitivity in Korean asthmatic patients. PMID: 21704099
  6. produced by trophoblast cells PMID: 19665237
  7. The most common defect, which leads to an increased susceptibility to Neisseria meningitidis infections in the Western Cape, is 879delG. PMID: 17257682

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Involvement in disease Complement component 6 deficiency (C6D)
Subcellular Location Secreted.
Protein Families Complement C6/C7/C8/C9 family
Database Links

HGNC: 1339

OMIM: 217050

KEGG: hsa:729

STRING: 9606.ENSP00000263413

UniGene: Hs.481992

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