Human Corin ELISA Kit

Code CSB-E17447h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9Y5Q5
Lead Time 3-5 working days
Abbreviation CORIN
Target Name corin, serine peptidase
Alias ATC2, CRN, Lrp4, MGC119742, TMPRSS10, atrial natriuteric peptide-converting enzyme|corin|corin, serine protease|heart specific serine proteinase|pro-ANP-convertase|pro-ANP-converting enzyme
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase.
HGNC 19012
RGD 727887
MGI 1349451
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Corin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 87    
Range % 83-95    
1:2 Average % 98    
Range % 94-102    
1:4 Average % 102    
Range % 98-106    
1:8 Average % 95    
Range % 90-100    
Recovery
The recovery of human Corin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 91 85-104    
EDTA plasma (n=4) 104 88-108    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
40 2.529 2.625 2.577 2.425    
20 2.007 2.121 2.064 1.912    
10 1.235 1.245 1.240 1.088    
5 0.744 0.757 0.751 0.599    
2.5 0.428 0.455 0.442 0.290    
1.25 0.284 0.291 0.288 0.136    
0.625 0.233 0.245 0.239 0.087    
0 0.150 0.154 0.152      
References
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Function Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide.; FUNCTION
Involvement in disease Pre-eclampsia/eclampsia 5 (PEE5)
Subcellular Location Cell membrane, Single-pass type II membrane protein, Note=May easily detached from the endothelial cell membrane, SUBCELLULAR LOCATION: Isoform 2: Cell membrane, Single-pass type II membrane protein, Note=Less efficiently targeted to the cell membrane compared to isoform 1, SUBCELLULAR LOCATION: Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment: Secreted, Note=Soluble form produced following cleavage by ADAM10, SUBCELLULAR LOCATION: Atrial natriuretic peptide-converting enzyme, 160 kDa soluble fragment: Secreted, Note=Soluble form produced following autocatalytic cleavage, SUBCELLULAR LOCATION: Atrial natriuretic peptide-converting enzyme, 100 kDa soluble fragment: Secreted
Protein Families Peptidase S1 family
Tissue Specificity Highly expressed in heart. Expressed in heart myocytes. Also expressed in pregnant uterus. Detected in blood, in plasma as well as in serum (at protein level).
Database Links

HGNC: 19012

OMIM: 605236

KEGG: hsa:10699

STRING: 9606.ENSP00000273857

UniGene: Hs.518618

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