Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

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Customer Reviews and Q&A

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Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Citations (8) Protocol MSDS

Code	CSB-E05140h
Size	96T,5×96T,10×96T
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Product Details
Citations (8)
Related Products
Customer Reviews and Q&A

Target Name

Creatine Kinase MB isoenzyme,CK-MB

Alternative Names

N/A

Abbreviation

CK-MB

Species

Homo sapiens (Human)

Sample Types

serum, plasma, urine, tissue homogenates

Detection Range

0.9 ng/mL-60 ng/mL

Sensitivity

0.22 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	94
	Range %	89-100
1:4	Average %	98
	Range %	91-104
1:8	Average %	106
	Range %	102-112

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
60	3.334	3.234	3.284	3.168
30	2.152	2.067	2.110	1.994
15	1.214	1.168	1.191	1.075
7.5	0.743	0.729	0.736	0.620
3.75	0.443	0.451	0.447	0.331
1.8	0.289	0.273	0.281	0.165
0.9	0.198	0.187	0.192	0.077
0	0.113	0.118	0.116

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human CK-MB ELISA Kit was designed for the quantitative measurement of Human CK-MB protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.22 ng/mL.

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Moderate aerobic exercise improves haematological indices without altering cardio-metabolic enzyme activities in sedentary healthy young adults IA Okon, JA Beshel, DU Owu, NN Orie, AE Jim,BMC Sports Science, Medicine and Rehabilitation,2025
Up-regulated fatty acid-binding protein 4 promoted cardiac injury during open colorectal surgery in elderly patients W Shi, S Li, Q Liu, Y Ji, X Weng, J Du,Journal of Visceral Surgery,2024
PPARA ameliorates sepsis-induced myocardial injury via promoting macrophage M2 polarization by interacting with DUSP1 L Cheng,Regenerative therapy,2024
Nontargeted Metabolomic Profiling of Huo-Tan-Chu-Shi Decoction in the Treatment of Coronary Heart Disease with Phlegm-damp Syndrome Z Liang,Cardiology research and practice,2022
ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis H Jiang,Biomedicine & pharmacotherapy,2022
Amelioration of ER stress by 4-phenylbutyric acid reduces chronic hypoxia induced cardiac damage and improves hypoxic tolerance through upregulation of HIF-1a Jain K.et al,Vascul Pharmacol.,2016

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.