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Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Citations (4) Protocol MSDS

Code	CSB-E05140h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details
Citations (4)
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Product Details

Target Name

Creatine Kinase MB isoenzyme,CK-MB

Alternative Names

N/A

Abbreviation

CK-MB

Species

Homo sapiens (Human)

Sample Types

serum, plasma, urine, tissue homogenates

Detection Range

0.9 ng/mL-60 ng/mL

Sensitivity

0.22 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-90
1:2	Average %	104
	Range %	101-107
1:4	Average %	98
	Range %	94-102
1:8	Average %	101
	Range %	98-104

Recovery

The recovery of human CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	109	104-113

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.016	2.106	2.061	1.907
30	1.646	1.604	1.625	1.471
15	1.215	1.253	1.234	1.080
7.5	0.859	0.848	0.854	0.700
3.75	0.586	0.552	0.569	0.415
1.8	0.370	0.367	0.369	0.215
0.9	0.284	0.278	0.281	0.127
0	0.155	0.153	0.154

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human CK-MB ELISA Kit was designed for the quantitative measurement of Human CK-MB protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.22 ng/mL.

Citations

PPARA ameliorates sepsis-induced myocardial injury via promoting macrophage M2 polarization by interacting with DUSP1 L Cheng,Regenerative therapy,2024
Nontargeted Metabolomic Profiling of Huo-Tan-Chu-Shi Decoction in the Treatment of Coronary Heart Disease with Phlegm-damp Syndrome Z Liang,Cardiology research and practice,2022
ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis H Jiang,Biomedicine & pharmacotherapy,2022
Amelioration of ER stress by 4-phenylbutyric acid reduces chronic hypoxia induced cardiac damage and improves hypoxic tolerance through upregulation of HIF-1a Jain K.et al,Vascul Pharmacol.,2016

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Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A