Rabbit creatine kinase MB isoenzyme (CK-MB) ELISA kit

Code CSB-EQ027653RB
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name creatine kinase MB isoenzyme (CK-MB)
Abbreviation CK-MB
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 mU/mL-100 mU/mL
Sensitivity 0.39 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %89
Range %84-93
1:2Average %95
Range %90-99
1:4Average %94
Range %89-100
1:8Average %102
Range %96-107
The recovery of rabbit CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8883-96
EDTA plasma (n=4)9288-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
1002.889 2.984 2.937 2.833
502.435 2.566 2.501 2.397
251.815 1.923 1.869 1.765
12.51.012 1.027 1.020 0.916
6.250.512 0.506 0.509 0.405
3.120.324 0.311 0.318 0.214
1.560.181 0.178 0.180 0.076
00.104 0.103 0.104
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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