Canine Creatine Kinase MB isoenzyme (CK-MB)ELISA Kit

Instructions
Code CSB-E15852c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Creatine Kinase MB isoenzyme (CK-MB)
Abbreviation CK-MB
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma
Detection Range 1.56 μIU/mL-100 μIU/mL
Sensitivity 0.78 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 84
Range % 80-88
1:200 Average % 98
Range % 94-102
1:400 Average % 90
Range % 86-94
1:800 Average % 92
Range % 88-96
Recovery
The recovery of canine CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 100 95-107
EDTA plasma (n=4) 89 85-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average
100 0.157 0.162 0.160
50 0.301 0.284 0.293
25 0.464 0.491 0.478
12.5 0.736 0.715 0.726
6.25 0.982 0.959 0.971
3.12 1.275 1.307 1.291
1.56 1.513 1.563 1.538
0 2.029 2.126 2.078
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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