Human Decorin/Bone proteoglycan II (DCN) ELISA Kit

Code CSB-E16522h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P07585
Lead Time 3-5 working days
Abbreviation DCN
Target Name decorin
Alias CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B, bone proteoglycan II|decorin proteoglycan|dermatan sulphate proteoglycans II|proteoglycan core protein|small leucine-rich protein 1B
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates_x000D_
Detection Range 3.9 pg/mL-250 pg/mL
Sensitivity 0.97 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome.
HGNC 2705
RGD 61895
MGI 94872
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:10 Average % 95    
Range % 91-99    
1:20 Average % 103    
Range % 98-106    
1:40 Average % 110    
Range % 106-114    
1:80 Average % 92    
Range % 89-97    
Recovery
The recovery of human DCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 84 81-89    
EDTA plasma (n=4) 97 93-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
250 1.944 1.968 1.956 1.815    
125 1.284 1.232 1.258 1.117    
62.5 0.750 0.771 0.761 0.620    
31.2 0.512 0.502 0.507 0.366    
15.6 0.374 0.368 0.371 0.230    
7.8 0.258 0.262 0.260 0.119    
3.9 0.194 0.199 0.197 0.056    
0 0.140 0.142 0.141      
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Function May affect the rate of fibrils formation.
Involvement in disease Corneal dystrophy, congenital stromal (CSCD)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Small leucine-rich proteoglycan (SLRP) family, SLRP class I subfamily
Database Links

HGNC: 2705

OMIM: 125255

KEGG: hsa:1634

STRING: 9606.ENSP00000052754

UniGene: Hs.156316

Pathway TGF-beta signaling pathway

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