Product Details

Target Name

deoxyribonuclease I-like 3

Alternative Names

Abbreviation

DNASE1L3

Uniprot No.

Q13609

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

23.44 pg/mL-1500 pg/mL

Sensitivity

5.86 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DNASE1L3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Recovery

The recovery of human DNASE1L3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.634	2.538	2.586	2.500
750	2.223	2.302	2.263	2.177
375	1.817	1.835	1.826	1.740
187.5	1.117	1.204	1.161	1.075
93.75	0.759	0.748	0.754	0.668
46.88	0.484	0.498	0.491	0.405
23.44	0.277	0.261	0.269	0.183
0	0.087	0.085	0.086

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

5-7 working days

Still Have Questions?

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Target Data

Function	Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA
Involvement in disease	Systemic lupus erythematosus 16 (SLEB16)
Subcellular Location	Nucleus, Endoplasmic reticulum, Secreted
Protein Families	DNase I family
Tissue Specificity	Liver and spleen.
Database Links	HGNC: 2959 OMIM: 602244 KEGG: hsa:1776 STRING: 9606.ENSP00000316193 UniGene: Hs.476453

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Human Deoxyribonuclease gamma(DNASE1L3) ELISA kit

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Code	CSB-EL007052HU
Size	96T,5×96T,10×96T