Human Deoxyribonuclease gamma(DNASE1L3) ELISA kit

Instructions
Code CSB-EL007052HU
Size 96T,5×96T,10×96T

Product Details

Target Name deoxyribonuclease I-like 3
Alternative Names DHP2, DNAS1L3, LSD, DNase I homolog protein 2|DNase gamma|LS-DNase|Liver and spleen DNase|deoxyribonuclease I-like III|deoxyribonuclease gamma
Abbreviation DNASE1L3
Uniprot No. Q13609
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DNASE1L3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 86-95
1:2 Average % 102
Range % 97-107
1:4 Average % 91
Range % 85-97
1:8 Average % 97
Range % 91-103
Recovery
The recovery of human DNASE1L3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-98
EDTA plasma (n=4) 97 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.634 2.538 2.586 2.500
750 2.223 2.302 2.263 2.177
375 1.817 1.835 1.826 1.740
187.5 1.117 1.204 1.161 1.075
93.75 0.759 0.748 0.754 0.668
46.88 0.484 0.498 0.491 0.405
23.44 0.277 0.261 0.269 0.183
0 0.087 0.085 0.086  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA
Involvement in disease Systemic lupus erythematosus 16 (SLEB16)
Subcellular Location Nucleus, Endoplasmic reticulum, Secreted
Protein Families DNase I family
Tissue Specificity Liver and spleen.
Database Links

HGNC: 2959

OMIM: 602244

KEGG: hsa:1776

STRING: 9606.ENSP00000316193

UniGene: Hs.476453

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