Product Details

Description

This Human DNASE1L3 ELISA Kit was designed for the quantitative measurement of Human DNASE1L3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

Target Name

deoxyribonuclease I-like 3

Alternative Names

Deoxyribonuclease gamma ELISA Kit; Deoxyribonuclease I like 3 ELISA Kit; Deoxyribonuclease I like III ELISA Kit; Deoxyribonuclease I-like 3 ELISA Kit; DHP 2 ELISA Kit; DHP2 ELISA Kit; DNAS1L3 ELISA Kit; DNase gamma ELISA Kit; DNase I homolog protein 2 ELISA Kit; DNase I homolog protein DHP2 ELISA Kit; DNase I like 3 ELISA Kit; DNase I-like 3 ELISA Kit; DNASE1L3 ELISA Kit; DNSL3_HUMAN ELISA Kit; Liver and spleen DNase ELISA Kit; LS DNase ELISA Kit; LS-DNase ELISA Kit; LSD ELISA Kit; SLEB 16 ELISA Kit; SLEB16 ELISA Kit

Abbreviation

DNASE1L3

Uniprot No.

Q13609

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

23.44 pg/mL-1500 pg/mL

Sensitivity

5.86 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DNASE1L3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Recovery

The recovery of human DNASE1L3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.634	2.538	2.586	2.500
750	2.223	2.302	2.263	2.177
375	1.817	1.835	1.826	1.740
187.5	1.117	1.204	1.161	1.075
93.75	0.759	0.748	0.754	0.668
46.88	0.484	0.498	0.491	0.405
23.44	0.277	0.261	0.269	0.183
0	0.087	0.085	0.086

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Related Products

Target Background

Function (From Uniprot)	Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells. Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity. Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
Gene References into Functions	The authors found a significant reduction in serum DNase1l3 level in dermatomyositis/polymyositis and systemic lupus erythematosus, which may associate with clinic features and disease activity. PMID: 28039554 Data indicate a likely functional variant influencing scleroderma susceptibility at deoxyribonuclease I-like 3 protein DNASE1L3 missense polymorphism rs35677470. PMID: 25332064 Single nucleotide polymorphisms in the DNASE1L3 is associated with autoimmune diseases. PMID: 24206041 DNASE1L3 mutations occur in hypocomplementemic urticarial vasculitis syndrome. PMID: 23666765 We identified a rare autosomal recessive form of systemic lupus erythematosus, in which autozygome analysis revealed a null mutation in the DNASE1L3 gene PMID: 22019780 investigates the distribution of DNASE1L3 gene SNPs in exons of the gene in eight Asian, three African, and three Caucasian populations worldwide using newly devised genotyping methods. PMID: 21692081 The Poly(ADP-ribose) polymerase-1-regulated endonuclease DNAS1L3 is required for etoposide-induced internucleosomal DNA fragmentation and increases etoposide cytotoxicity in transfected osteosarcoma cells. PMID: 12154052 Identification of two functional nuclear localization signals in DNase gamma that have a role in apoptotic DNA fragmentation. PMID: 12943533 DNase gamma is involved in the generation of resected double-strand DNA breaks associated with somatic hypermutation PMID: 15629432 DNAS1L3 plays an active role in lymphoma cell sensitization to VP-16 and its deficiency may constitute a novel mechanism of drug resistance in these cells PMID: 16427601 A Caucasian-specific allele in SNP R206C produces an inactive form of DNase Il3. PMID: 19559017 Results distinguish DNase gamma-dependent and CAD/DFF40-dependent DNA fragmentations, and show that even if DNA fragmentation is initiated by CAD/DFF40, DNase gamma is required for the more complete digestion of the genomic DNA in dying cells. PMID: 19574717 Show More Hide All
Involvement in disease	Systemic lupus erythematosus 16 (SLEB16)
Subcellular Location	Nucleus. Endoplasmic reticulum. Secreted.
Protein Families	DNase I family
Tissue Specificity	Liver and spleen.
Database Links	HGNC: 2959 OMIM: 602244 KEGG: hsa:1776 STRING: 9606.ENSP00000316193 UniGene: Hs.476453

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Code	CSB-EL007052HU
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Human Deoxyribonuclease gamma(DNASE1L3) ELISA kit

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Target Background

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