Human EGF-like repeat and discoidin I-like domain-containing protein 3(EDIL3) ELISA kit

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Target Background

Name: Human EGF-like repeat and discoidin I-like domain-containing protein 3(EDIL3) ELISA kit
Brand: CUSABIO
SKU: CSB-EL007399HU

Product Details

Target Name

EGF-like repeats and discoidin I-like domains 3

Alternative Names

DEL1 ELISA Kit; Developmental endothelial locus 1 ELISA Kit; Developmentally regulated endothelial cell locus 1 protein ELISA Kit; Developmentally-regulated endothelial cell locus 1 protein ELISA Kit; Edil3 ELISA Kit; EDIL3_HUMAN ELISA Kit; EGF like repeat and discoidin I like domain containing protein 3 ELISA Kit; EGF like repeats and discoidin I like domains 3 ELISA Kit; EGF like repeats and discoidin I like domains protein 3 ELISA Kit; EGF-like repeat and discoidin I-like domain-containing protein 3 ELISA Kit; Integrin binding protein DEL1 ELISA Kit; Integrin-binding protein DEL1 ELISA Kit

Abbreviation

EDIL3

Uniprot No.

O43854

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EDIL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-95
1:2	Average %	95
	Range %	89-100
1:4	Average %	99
	Range %	91-110
1:8	Average %	94
	Range %	88-98

Recovery

The recovery of human EDIL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	80-98
EDTA plasma (n=4)	96	92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.033	1.872	1.953	1.765
4000	1.598	1.476	1.537	1.349
2000	1.077	1.014	1.046	0.858
1000	0.774	0.729	0.752	0.564
500	0.513	0.494	0.504	0.316
250	0.374	0.359	0.367	0.179
125	0.291	0.278	0.285	0.097
0	0.190	0.186	0.188

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The product CSB-EL007399HU is a sandwich ELISA kit developed to measure concentrations of human EDIL3 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of EDIL3 in samples. The color intensity is measured at 450 nm via a microplate reader.

As a pro-angiogenic factor, EDIL3 mediates the immune and anti-inflammatory response. EDIL3 acts as a regulator of endothelial cell adhesion and migration. Additionally, Edil3 has been described as an autocrine or paracrine secreted protein that participates in endothelial cell migration, angiogenesis, and branching morphogenesis of blood vessels. It has been reported that EDIL3 is overexpressed in several tumor types, including bladder, pancreas, breast, and liver carcinomas. Overexpression of EDIL3 promotes carcinogenesis by reducing apoptosis in cancer cells and stimulating cancer vascularization. It is associated with tumor progression and poor prognosis.

Target Background

Function
(From Uniprot)

Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.

Gene References into Functions

EDIL3 may be implicated in retinal neovascularization in vitro. Silencing EDIL3 expression was demonstrated to impair the proliferative, migratory and tube forming capabilities of HRECs in vitro. PMID: 28765888
these data reveal an unrecognised function of endogenous Del-1 as a local inhibitor of ischaemia-induced angiogenesis by restraining LFA-1-dependent homing of pro-angiogenic haematopoietic cells to ischaemic tissues PMID: 28447099
Del-1-induced HSC proliferation and myeloid lineage commitment were mediated by beta3 integrin on hematopoietic progenitors. PMID: 28846069
Del-1 pre-treatment of blood before addition of islets diminished coagulation activation and islet damage PMID: 26676803
Del-1 on circulating EVs is a promising marker to improve identification of patients with early-stage breast cancer and distinguish breast cancer from benign breast tumors and noncancerous diseases. PMID: 26603257
Overexpressed EDIL3 promotes anchorage-independent tumor growth in human pancreatic cancer. PMID: 26735172
Overexpression of the Del-1 gene potentiates proliferation and invasion of lung carcinoma cells. PMID: 26545781
The importance of interaction between EDIL3 and integrin alphaV. PMID: 25273699
The reciprocal regulation between Del-1 and inflammation may contribute to optimally balance the protective and the potentially harmful effects of inflammatory cell recruitment. PMID: 24416060
Downregulation of developmentally regulated endothelial cell locus-1 inhibits the growth of colon cancer. PMID: 19292890
Del-1 may act as a gatekeeper of adrenal gland inflammation and may regulate the integrity of the hypothalamic-pituitary-adrenal axis stress response, thereby modulating adrenal (dys)function in the course of SIRS. PMID: 23364949
database search for EGF domain sequences shows that this RGD finger is likely an evolutionary insertion and unique to the EGF domain of Del-1 PMID: 22601780
Del-1 mediates phosphatidylserine- and integrin-dependent endothelial uptake of microparticles by endocytosis. PMID: 22388320
High expression level of EDIL3 predicts poor prognosis of hepatocellular carcinoma patients. PMID: 20857535
Del1 mediates vascular smooth muscle cell adhesion, migration, and proliferation through interaction with integrin alpha(v)beta(3). PMID: 11959660
acts as an angiogenic factor in the context of solid tumor formation; the increase in vascularization accelerates tumor growth through decreased apoptosis PMID: 12074641
Alpha v beta 5, alpha v beta 5 and their ligands Del-1 and L1 play an important role in the process of tumor cells moving from the original place. PMID: 18090124

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Subcellular Location

Secreted.

Database Links

HGNC: 3173

OMIM: 606018

KEGG: hsa:10085

STRING: 9606.ENSP00000296591

UniGene: Hs.482730

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Code	CSB-EL007399HU
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.