Human Fibroblast growth factor 17(FGF17) ELISA kit

Code CSB-EL008622HU
Size 96T,5×96T,10×96T
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Product Details

Alternative Names
FGF 13 ELISA Kit; FGF 17 ELISA Kit; FGF-17 ELISA Kit; FGF13 ELISA Kit; Fgf17 ELISA Kit; FGF17_HUMAN ELISA Kit; Fibroblast growth factor 17 ELISA Kit; HH20 ELISA Kit
Abbreviation
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell culture supernates.
Detection Range
3.12 pg/ml-200 pg/ml.
Sensitivity
0.78 pg/ml.
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:1

Average %

90

Range %

85-96

1:2

Average %

95

Range %

88-101

1:4

Average %

100

Range %

93-105

1:8

Average %

93

Range %

86-101

Recovery

The recovery of human FGF17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

95

86-100

EDTA plasma (n=4)

97

92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

200

2.264

2.234

2.249

2.078

100

1.582

1.667

1.625

1.454

50

0.986

1.028

1.007

0.836

25

0.660

0.629

0.645

0.474

12.5

0.453

0.431

0.442

0.271

6.25

0.338

0.343

0.341

0.170

3.12

0.251

0.244

0.247

0.077

0

0.166

0.175

0.171

 

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Target Background

Function
(From Uniprot)
Plays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development.
Gene References into Functions
  1. FGF17 and IL17RD prpopsed as the two top candidates in the entire proteome on the basis of a statistical test of their protein-protein interaction patterns to proteins known to be altered in congenital hypogonadotropic hypogonadism. PMID: 23643382
  2. FGF17 expression is increased 2-fold in benign prostatic hyperplasia and may contribute to the increased epithelial proliferation seen in this disease. PMID: 15129425
Involvement in disease
Hypogonadotropic hypogonadism 20 with or without anosmia (HH20)
Subcellular Location
Secreted.
Protein Families
Heparin-binding growth factors family
Tissue Specificity
Preferentially expressed in the embryonic brain.
Database Links

HGNC: 3673

OMIM: 603725

KEGG: hsa:8822

STRING: 9606.ENSP00000352414

UniGene: Hs.248192

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