Human Filaggrin(FLG) ELISA kit

Instructions
Code CSB-EL008712HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name filaggrin
Alternative Names ATOD2 ELISA Kit; Epidermal filaggrin ELISA Kit; FILA_HUMAN ELISA Kit; Filaggrin ELISA Kit; Filaggrin precursor ELISA Kit; Fillagrin ELISA Kit; FLG ELISA Kit; Profilaggrin ELISA Kit
Abbreviation FLG
Uniprot No. P20930
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-98  
1:2 Average % 95  
Range % 91-98  
1:4 Average % 99  
Range % 94-105  
1:8 Average % 105  
Range % 100-110  
Recovery
The recovery of human FLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 82-98  
EDTA plasma (n=4) 97 90-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1500 2.853 2.687 2.770 2.634  
750 2.465 2.343 2.404 2.268  
375 1.786 1.712 1.749 1.613  
187.5 0.895 0.865 0.880 0.744  
94 0.491 0.478 0.485 0.349  
47 0.299 0.288 0.294 0.158  
23.5 0.212 0.208 0.210 0.074  
0 0.137 0.135 0.136    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
Gene References into Functions
  1. The present study demonstrated that the downregulation of filaggrin in the epidermis by toluene is mediated by ERK1/2 and STAT3-dependent pathways. PMID: 27498358
  2. FLG mutation was not associated with MM in the studied populations. PMID: 28833578
  3. The results show that patients carrying filaggrin mutations had increase prevalence of Staphylococcus aureus colonization. PMID: 28317091
  4. We suggest that SNP in FLG (rs11204981) may serve as an important predictive marker for the combined eczema plus asthma phenotype, and that the highest level of expression in heterozygous may have a protective role in developing allergy phenotype. PMID: 29569866
  5. This study indicates an increased susceptibility to actinic keratosis in individuals with homozygous, but not heterozygous, FLG mutations and in patients with atopic dermatitis compared to psoriasis. PMID: 28213896
  6. This study demonstrated, for the first time, that FLG expression in UCB is associated with eczema development in infancy. Moreover, our analysis provided prediction models that were capable of discriminating, to a great extent, between those who will and will not develop eczema in infancy. PMID: 28502108
  7. In a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC. both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations PMID: 27805415
  8. FLG and POSTN expression may be downregulated and upregulated, respectively, in the esophageal mucosa of patients with active eosinophilic esophagitis, and these changes may be restored with treatment in a significant percentage of cases. PMID: 28644349
  9. immunoreactivity for filaggrin was significantly more intense in the oral mucosa in the patients with OLP/OLL compared with healthy controls PMID: 28000306
  10. atopic dermatitis patients without palmoplantar hyperlinearity unlikely to carry FLG mutations PMID: 27120251
  11. FLG mutations are strongly associated with atopic eczema and confer a significant risk of allergic sensitization and asthma in the context of eczema in Polish children. PMID: 29068602
  12. There was no association of the atopy risk variants in the FLG gene with OFG. PMID: 27306066
  13. suggest that FLG P478S is a kind of disease modifier which affects serologic parameters such as EDN and ECP PMID: 29281699
  14. In this study of 97 174 individuals from the Danish general population, FLG loss-of-function mutations were associated with increased ischemic stroke risk PMID: 28164424
  15. study found that among patients with atopic dermatitis (AD), common FLG null mutations are associated with earlier AD onset in a dose-dependent manner, whereas TSLP rs1898671 appears unrelated to the timing of AD onset PMID: 28479194
  16. The two SNPs K4671X and rs3126085 of FLG were related to Epstein-Barr virus (EBV)-associated gastric carcinoma. PMID: 28455573
  17. FLG mutation is associated with IgE sensitization to peanut but not to other allergens in Swedish children up to 16 years of age PMID: 28456621
  18. Erythemal doses of ultraviolet B exert acute effects on profilaggrin mRNA and filaggrin protein in human skin in vivo. PMID: 28358172
  19. FLG mutations are associated with early onset of atopic dermatitis, more severe clinical course of disease, and a significantly increased risk ofMolluscum contagiosum sustained skin infection PMID: 28866311
  20. Study conducted in Croatia found a low frequency of FLG null-mutations in general population (2.6%) and did not confirm FLG null-mutations as an etiological factor for Atopy and Atopic disease in the studied population. PMID: 29087092
  21. women with FLG mutations may have an increased risk of AD flares during pregnancy and of enduring postpartum problems attributable to perineal trauma during delivery. PMID: 26835886
  22. FLG mutations are risk factors for atopic dermatitis in Finns, but disease severity and treatment response were independent of patient FLG status. PMID: 27840886
  23. Patients with both atopic dermatitis and common filaggrin gene mutations are more frequently affected by reduced health-related quality of life PMID: 27995642
  24. Multiple lines of evidence suggest that FLG genetic variation, have little or no effect on fitness in modern humans. Haplotype-level analysis revealed a recent selective sweep which increased the allele frequency of the Huxian haplogroup in Asian populations. PMID: 27678121
  25. FLG mutations lead to alterations in epidermal eicosanoid metabolism in atopic dermatitis patients PMID: 27793761
  26. Filaggrin gene mutations are risk factors for the presence and persistence of atopic dermatitis and explain the discordance of atopic dermatitis within dizygotic twin pairs. PMID: 27653621
  27. Variations in FLG and TSLP genotype were associated with differences in self-reported skin clearance, TCI usage, and steroid usage. PMID: 27902816
  28. We show that subjects with FLG-null mutations have more mature Langerhans cells in nonlesional skin irrespective of whether they have AD. PMID: 26934939
  29. we have proposed that this latitude-dependent gradient of FLG mutations across Europe, Asia and Africa could have provided an evolutionary advantage for heterozygous FLG mutation carriers, residing at northern latitudes, depletion of the FLG downstream product, trans-urocanic acid, would facilitate the intracutaneous synthesis of vitamin D3 by allowing increased transcutaneous absorption of UVB photons PMID: 28338939
  30. The results of the present study demonstrate that filaggrin knockdown inhibits NHEK migration, adhesion and proliferation, promotes apoptosis and disturbs cell cycle progression. PMID: 27485743
  31. FLG rs2065955 polymorphism was significantly related to gastric carcinoma. PMID: 27535066
  32. Four FLG null mutations (3321delA, K4022X, S3296X, and S2889X) were identified in Korean patients with Atopic Dermatitis PMID: 28120571
  33. IL-33, which is a representative Th2 cytokine, affect as a crucial factor of skin barrier dysfunction by reducing FLG expression in human keratinocytes. PMID: 26867960
  34. The authors data reveals that the CNV of FLG is associated with AD development in Koreans. PMID: 26554544
  35. Filaggrin Gene Mutation c.3321delA is Associated with Dry Phenotypes of Atopic Dermatitis in the Chinese Han Population PMID: 27270549
  36. A mutation p.Y1767X, was related to early onset and severe symptoms, which last until adulthood, and severe atopic dermatitis with other atopic diseases, such as asthma. PMID: 27366014
  37. The present study examined the possible relationship between SNPs of FLG and chronic idiopathic urticaria (CIU) for the first time, and demonstrated that none of five investigated SNPs (rs2485518, rs3126065, rs2786680, rs3814300, and rs3814299) are correlated with CIU in an Iranian population. PMID: 26796858
  38. The study revealed 66 FLG mutation carriers and demonstrated an association between c.2282del4 deletion and atopic dermatitis development in Russians and Tatars of Volga-Ural region of Russia. PMID: 27363669
  39. The levels of filaggrin, inflammatory T helper 2 polarizing cytokines (thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL-33)) and chemokine (C-C motif) ligand 27 (CCL27), histological severity markers, T and dendritic cell counts in biopsies from lesional skin of severe atopic dermatitis patients with and without filaggrin mutation and healthy skin were quantified. No significant differences were found. PMID: 26536977
  40. The imbalance between Th1 and Th2 polarized immune response seems to extend to Filaggrin homeostasis, through the network of Filaggrin processing enzymes. PMID: 26831231
  41. Levels of FLG, FLG2 and SPRR3 mRNAs and proteins were reduced in AD skin. PMID: 27304082
  42. Data suggest that the c.1360A>G (p.T454A) and c.10363G>T (p.D3455Y) mutations of the filaggrin (FLG) gene may lead to alteration of the structure and function of the FLG protein and cause ichthyosis vulgaris in the two families. PMID: 27577213
  43. genotype-phenotype correlation not obvious in Korean atopic dermatitis patients with null mutations PMID: 25997159
  44. Results show a strong association of FLG loss-of-function mutations was found with doctor-diagnosed CD and to a lesser extent also with self-reported CD, but not with respiratory symptoms or atopy. PMID: 26451970
  45. Identify acefylline as an activator of peptidylarginine deiminase 1 and 3 in the epidermis, resulting in filaggrin deimination. PMID: 26616205
  46. FLG has been, and still remains the major susceptibility gene in patients with AD. PMID: 25580797
  47. Letter: Knockdown of either filaggrin or loricrin increases the productions of interleukin (IL)-1alpha, IL-8, IL-18 and granulocyte macrophage colony-stimulating factor in stratified human keratinocytes. PMID: 26381575
  48. Supplementation with the PPARalpha agonist WY14643 improved the homeostasis and barrier function of filaggrin deficient skin models by normalization of the free fatty acid profile. PMID: 26472199
  49. Filaggrin genotype does not determine the skin's threshold to UV-induced erythema. PMID: 26830116
  50. In Iranian patients, this study demonstrated that there was no significant association between polymorphisms of FLG gene variants and AD. PMID: 25230061

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Involvement in disease Ichthyosis vulgaris (VI); Dermatitis atopic 2 (ATOD2)
Subcellular Location Cytoplasmic granule
Protein Families S100-fused protein family
Tissue Specificity Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal
Database Links

HGNC: 3748

OMIM: 135940

KEGG: hsa:2312

STRING: 9606.ENSP00000357789

UniGene: Hs.654510

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