Target Name
lectin, galactoside-binding, soluble, 8
Alternative Names
Gal 8 ELISA Kit; Gal-8 ELISA Kit; Gal8 ELISA Kit; Galectin 8 ELISA Kit; Galectin-8 ELISA Kit; galectin-8g ELISA Kit; Lectin galactoside binding soluble 8 ELISA Kit; LEG8_HUMAN ELISA Kit; LGAL S8 ELISA Kit; Lgals8 ELISA Kit; PCTA 1 ELISA Kit; PCTA-1 ELISA Kit; PCTA1 ELISA Kit; Po66 carbohydrate binding protein ELISA Kit; Po66 carbohydrate-binding protein ELISA Kit; Po66 CBP ELISA Kit; Po66-CBP ELISA Kit; Prostate carcinoma tumor antigen 1 ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | | |
Inter-assay Precision (Precision between assays): CV%<10% | | | | |
Three samples of known concentration were tested in twenty assays to assess. | | | |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| Sample | Serum(n=4) | | |
1:1 | Average % | 95 | | |
Range % | 87-99 | | |
1:2 | Average % | 87 | | |
Range % | 83-92 | | |
1:4 | Average % | 90 | | |
Range % | 84-93 | | |
1:8 | Average % | 99 | | |
Range % | 91-103 | | |
Recovery
The recovery of human LGALS8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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Sample Type | Average % Recovery | Range | | |
Serum (n=5) | 87 | 81-93 | | |
EDTA plasma (n=4) | 95 | 88-102 | | |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.012 | 1.986 | 1.999 | 1.826 | | | 1000 | 1.597 | 1.535 | 1.566 | 1.393 | | | 500 | 1.106 | 1.067 | 1.087 | 0.914 | | | 250 | 0.833 | 0.802 | 0.818 | 0.645 | | | 125 | 0.521 | 0.514 | 0.518 | 0.345 | | | 62.5 | 0.384 | 0.379 | 0.382 | 0.209 | | | 31.25 | 0.279 | 0.272 | 0.276 | 0.103 | | | 0 | 0.175 | 0.171 | 0.173 | | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Human LGALS8 ELISA Kit was designed for the quantitative measurement of Human LGALS8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.