Human Ganglioside GM2 activator(GM2A) ELISA kit

Code CSB-EL009565HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
GM2 ganglioside activator
Alternative Names
Cerebroside sulfate activator protein ELISA Kit; ganglioside GM2 activator ELISA Kit; Ganglioside GM2 activator isoform short ELISA Kit; Ganglioside GM2 activator precursor ELISA Kit; GM2 activator ELISA Kit; GM2 AP ELISA Kit; GM2 ganglioside activator ELISA Kit; GM2 ganglioside activator protein ELISA Kit; GM2-AP ELISA Kit; GM2A ELISA Kit; GM2AP ELISA Kit; OTTHUMP00000160619 ELISA Kit; SAP 3 ELISA Kit; SAP-3 ELISA Kit; SAP3 ELISA Kit; SAP3_HUMAN ELISA Kit; Shingolipid activator protein 3 ELISA Kit; Sphingolipid activator protein 3 ELISA Kit
Abbreviation
GM2A
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, tissue homogenates, cell lysates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GM2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %84-95
1:2Average %92
Range %87-99
1:4Average %100
Range %95-106
1:8Average %97
Range %92-102
Recovery
The recovery of human GM2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-103
EDTA plasma (n=4)9084-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.043 2.050 2.047 1.921
5001.619 1.684 1.652 1.526
2501.105 1.200 1.153 1.027
1250.683 0.703 0.693 0.567
62.50.403 0.421 0.412 0.286
31.20.296 0.322 0.309 0.183
15.60.241 0.235 0.238 0.112
00.123 0.129 0.126  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human GM2A ELISA Kit was designed for the quantitative measurement of Human GM2A protein in serum, plasma, urine, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function
(From Uniprot)
The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity. Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. Has cholesterol transfer activity.
Gene References into Functions
  1. Review of GM2A mutations causing GM2 activator protein deficiency and GM2 gangliosidosis-AB variant. PMID: 27402091
  2. Studies indicate that sphingolipid activator proteins (SAPs) and anionic lipids are essential stimulators to reach physiological rates of lysosomal sphingolipid degradation. PMID: 27157270
  3. this study has established the potential role of GM2A in breast cancer progression PMID: 27002480
  4. Mobilization of membrane lipids by GM2AP was also inhibited in the presence of cholesterol or SM, as revealed by surface plasmon resonance studies PMID: 26175473
  5. Gene polymorphisms of MD2 and GM2A were associated with the occurrence or severity of neonatal necrotizing enterocolitis. PMID: 25816011
  6. In vitro assays with the isolated H1 or H2 homodimers (beta-alpha hybrid construct of beta-hexosaminidase A subunits) confirmed that neither was capable of human GM2AP-dependent hydrolysis of GM2 ganglioside. PMID: 23483939
  7. impact of GM2AP on glucose metabolism PMID: 21784073
  8. Treatment of meniscal explants with IL-1RA inhibited the expression of many catabolic genes following a single bout of high dynamic strain. PMID: 21331553
  9. these results provide novel insights into the physiological functions of GM2AP in obesity. PMID: 21036149
  10. GM2 activator protein exerts strong and broad inhibitory effects on the hydrolysis of phospholipids carried out by plant and microbial phospholipases D PMID: 12576516
  11. Two new structures of GM2-AP with bound lipids, showing two different lipid-binding modes within the apolar pocket PMID: 12909021
  12. elucidation of mode of action on gangioside GM2 PMID: 14728689
  13. alpha-subunit loop structure is required for GM2 activator protein binding by beta-hexosaminidase A PMID: 15485660
  14. glycosphingolipids, particularly GM2, form a complex with CD82, and this complex interacts with Met and thereby inhibits HGF-induced Met tyrosine kinase activity, as well as integrin to Met cross-talk PMID: 17215249

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Involvement in disease
GM2-gangliosidosis AB (GM2GAB)
Subcellular Location
Lysosome.
Database Links

HGNC: 4367

OMIM: 272750

KEGG: hsa:2760

STRING: 9606.ENSP00000349687

UniGene: Hs.483873

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