GM2A Antibody

Datasheet

Code	CSB-PA02379A0Rb
Size	US$166
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Image	Western blot All lanes: GM2A antibody at 10µg/ml Lane 1: Rat liver tissue Lane 2: Mouse stomach tissue Lane 3: Mouse small intestine tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA02379A0Rb at dilution of 1:100 Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA02379A0Rb at dilution of 1:100 Immunoprecipitating GM2A in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA02379A0Rb in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA02379A0Rb (8µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (10µg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GM2A Polyclonal antibody

Uniprot No.

P17900

Target Names

GM2A

Alternative Names

Cerebroside sulfate activator protein antibody; ganglioside GM2 activator antibody; Ganglioside GM2 activator isoform short antibody; Ganglioside GM2 activator precursor antibody; GM2 activator antibody; GM2 AP antibody; GM2 ganglioside activator antibody; GM2 ganglioside activator protein antibody; GM2-AP antibody; GM2A antibody; GM2AP antibody; OTTHUMP00000160619 antibody; SAP 3 antibody; SAP-3 antibody; SAP3 antibody; SAP3_HUMAN antibody; Shingolipid activator protein 3 antibody; Sphingolipid activator protein 3 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Ganglioside GM2 activator protein (32-193AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GM2A Antibody (Product code: CSB-PA02379A0Rb) is Non-conjugated. For GM2A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA02379B0Rb	GM2A Antibody, HRP conjugated	ELISA
FITC	CSB-PA02379C0Rb	GM2A Antibody, FITC conjugated
Biotin	CSB-PA02379D0Rb	GM2A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity. Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. Has cholesterol transfer activity.

Gene References into Functions

Review of GM2A mutations causing GM2 activator protein deficiency and GM2 gangliosidosis-AB variant. PMID: 27402091
Studies indicate that sphingolipid activator proteins (SAPs) and anionic lipids are essential stimulators to reach physiological rates of lysosomal sphingolipid degradation. PMID: 27157270
this study has established the potential role of GM2A in breast cancer progression PMID: 27002480
Mobilization of membrane lipids by GM2AP was also inhibited in the presence of cholesterol or SM, as revealed by surface plasmon resonance studies PMID: 26175473
Gene polymorphisms of MD2 and GM2A were associated with the occurrence or severity of neonatal necrotizing enterocolitis. PMID: 25816011
In vitro assays with the isolated H1 or H2 homodimers (beta-alpha hybrid construct of beta-hexosaminidase A subunits) confirmed that neither was capable of human GM2AP-dependent hydrolysis of GM2 ganglioside. PMID: 23483939
impact of GM2AP on glucose metabolism PMID: 21784073
Treatment of meniscal explants with IL-1RA inhibited the expression of many catabolic genes following a single bout of high dynamic strain. PMID: 21331553
these results provide novel insights into the physiological functions of GM2AP in obesity. PMID: 21036149
GM2 activator protein exerts strong and broad inhibitory effects on the hydrolysis of phospholipids carried out by plant and microbial phospholipases D PMID: 12576516
Two new structures of GM2-AP with bound lipids, showing two different lipid-binding modes within the apolar pocket PMID: 12909021
elucidation of mode of action on gangioside GM2 PMID: 14728689
alpha-subunit loop structure is required for GM2 activator protein binding by beta-hexosaminidase A PMID: 15485660
glycosphingolipids, particularly GM2, form a complex with CD82, and this complex interacts with Met and thereby inhibits HGF-induced Met tyrosine kinase activity, as well as integrin to Met cross-talk PMID: 17215249

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Involvement in disease

GM2-gangliosidosis AB (GM2GAB)

Subcellular Location

Lysosome.

Database Links

HGNC: 4367

OMIM: 272750

KEGG: hsa:2760

STRING: 9606.ENSP00000349687

UniGene: Hs.483873

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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