Human Glucocorticoid receptor(NR3C1) ELISA kit

Code CSB-EL016059HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P04150
Lead Time 5-7 working days
Abbreviation NR3C1
Protein Biological Process 1 Transcription/Transcription regulation
Target Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Alias GCCR, GCR, GR, GRL, glucocorticoid receptor
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined.
HGNC 7978
RGD 2741
MGI 95824
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NR3C1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %82-95
1:2Average %92
Range %87-99
1:4Average %91
Range %85-97
1:8Average %87
Range %82-92
Recovery
The recovery of human NR3C1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-102
EDTA plasma (n=4)10696-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20001.724 1.742 1.733 1.600
10001.197 1.176 1.187 1.054
5000.763 0.753 0.758 0.625
2500.391 0.386 0.389 0.256
1250.306 0.287 0.297 0.164
62.50.236 0.244 0.240 0.107
31.250.189 0.179 0.184 0.051
00.132 0.134 0.133
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Function Receptor for glucocorticoids (GC)
Involvement in disease Glucocorticoid resistance, generalized (GCCR)
Subcellular Location Isoform Alpha: Cytoplasm, Nucleus, Mitochondrion, Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Note=After ligand activation, translocates from the cytoplasm to the nucleus, SUBCELLULAR LOCATION: Isoform Beta: Nucleus, Cytoplasm, Note=Expressed predominantly in the nucleus with some expression also detected in the cytoplasm, SUBCELLULAR LOCATION: Isoform Alpha-B: Nucleus, Cytoplasm
Protein Families Nuclear hormone receptor family, NR3 subfamily
Tissue Specificity Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney (PubMed:25847991). In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node
Database Links

HGNC: 7978

OMIM: 138040

KEGG: hsa:2908

STRING: 9606.ENSP00000231509

UniGene: Hs.122926

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