NR3C1, located on chromosome 5, encodes a glucocorticoid receptor that is widely distributed in the body. NR3C1 is closely related to the metabolism and immune response,etc. This receptor modulates gene transcription by binding to hormones like cortisol. Typically found in the cytosol, once bound to glucocorticoids, these receptor-hormone complexes can translocate to the nucleus, regulating gene expression through activation or inhibition. NR3C1 is pivotal in stress adaptation, offering insights into psychological disorders such as depression and PTSD. Moreover, its irregular expression is linked to various diseases, including autoimmune conditions, metabolic disorders, and certain types of cancer.
Recombinant Human Glucocorticoid receptor (NR3C1), partial (CSB-EP016059HU1c2)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
NR3C1 Recombinant Monoclonal Antibody (CSB-RA958910A0HU)
Validated Data
Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate
All lanes: NR3C1 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86, 83, 82, 77, 76, 65, 61, 52, 51, 50 kDa
Observed band size: 95 kDa
Overlay histogram showing Jurkat cells stained with CSB-RA958910A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Human Glucocorticoid receptor(NR3C1) ELISA kit (CSB-EL016059HU)
Validated Data
Code: CSB-EL016059HU
Size: 96T,5×96T,10×96T
Sensitivity: 7.81 pg/mL
Detection Range: 31.25 pg/mL-2000 pg/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following NR3C1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
NR3C1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA016059KA01HU | NR3C1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA298129 | Phospho-NR3C1 (Ser226) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA784840 | NR3C1 (Ab-211) Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
CSB-PA589113 | NR3C1 (Ab-226) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA000363 | NR3C1 Antibody | Human,Mouse,Rat | WB |
CSB-PA000689 | Phospho-NR3C1 (S211) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA008993 | Phospho-NR3C1 (S203) Antibody | Human,Mouse | WB, ELISA |
CSB-PA008994 | Phospho-NR3C1 (S226) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA008996 | NR3C1 Antibody | Human,Mouse | WB, IHC, ELISA |
CSB-PA008998 | NR3C1 Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA008999 | NR3C1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA866060 | NR3C1 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA225804 | NR3C1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA869503 | NR3C1 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA167985 | NR3C1 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA016059LA01HU | NR3C1 Antibody | Human | ELISA, WB, IHC, IF, IP |
CSB-PA016059LB01HU | NR3C1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA016059LC01HU | NR3C1 Antibody, FITC conjugated | Human | |
CSB-PA016059LD01HU | NR3C1 Antibody, Biotin conjugated | Human | ELISA |
CSB-RA958910A0HU | NR3C1 Recombinant Monoclonal Antibody | Human | ELISA, WB, FC |
NR3C1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP016059HU1 CSB-BP016059HU1 CSB-EP016059HU1-B |
Recombinant Human Glucocorticoid receptor (NR3C1), partial | Yeast Baculovirus In Vivo Biotinylation in E.coli |
CSB-EP016059HU1 | Recombinant Human Glucocorticoid receptor (NR3C1), partial | E.coli |
CSB-EP016059HU1c2 | Recombinant Human Glucocorticoid receptor (NR3C1), partial | E.coli |
CSB-EP016059HU1a2 | Recombinant Human Glucocorticoid receptor (NR3C1), partial | E.coli |
NR3C1 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL016059HU | Human Glucocorticoid receptor(NR3C1) ELISA kit | serum, plasma, tissue homogenates, cell lysates | 7.81 pg/mL |