NR3C1 Antibody

Datasheet
Code CSB-PA016059LA01HU
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Size US$299
Uniprot No. P04150
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate
    All lanes: NR3C1 antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 86, 82, 65, 61, 83, 79, 77, 76, 52, 51, 50 kDa
    Observed band size: 86 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA016059LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA016059LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Immunoprecipitating NR3C1 in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA016059LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
    Lane 2: CSB-PA016059LA01HU (8µg) + Hela whole cell lysate (500µg)
    Lane 3: Hela whole cell lysate (20µg)

Immunogen Recombinant Human Glucocorticoid receptor protein (1-190AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
Relevance Receptor for glucocorticoids (GC) (PubMed:27120390). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Glucocorticoid receptor (GR) (Nuclear receptor subfamily 3 group C member 1), NR3C1, GRL
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Research Area Signal Transduction
Target Names NR3C1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Receptor for glucocorticoids (GC)
Involvement in disease Glucocorticoid resistance, generalized (GCCR)
Subcellular Location Isoform Alpha: Cytoplasm, Nucleus, Mitochondrion, Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Note=After ligand activation, translocates from the cytoplasm to the nucleus, SUBCELLULAR LOCATION: Isoform Beta: Nucleus, Cytoplasm, Note=Expressed predominantly in the nucleus with some expression also detected in the cytoplasm, SUBCELLULAR LOCATION: Isoform Alpha-B: Nucleus, Cytoplasm
Protein Families Nuclear hormone receptor family, NR3 subfamily
Tissue Specificity Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney (PubMed:25847991). In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node
Database Links

HGNC: 7978

OMIM: 138040

KEGG: hsa:2908

STRING: 9606.ENSP00000231509

UniGene: Hs.122926

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