Human Gremlin-2(GREM2) ELISA kit

Product Details

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Target Background

Product Details

Target Name

gremlin 2, cysteine knot superfamily, homolog (Xenopus laevis)

Alternative Names

BMP antagonist 2 ELISA Kit; CKTSF1B2 ELISA Kit; Cysteine knot superfamily 1 ELISA Kit; Cysteine knot superfamily 1 BMP antagonist 2 ELISA Kit; DAN domain family member 3 ELISA Kit; DAND 3 ELISA Kit; DAND3 ELISA Kit; GREM 2 ELISA Kit; Grem2 ELISA Kit; GREM2_HUMAN ELISA Kit; Gremlin 2 ELISA Kit; Gremlin 2 cysteine knot superfamily homolog ELISA Kit; Gremlin-2 ELISA Kit; Gremlin2 ELISA Kit; PRDC ELISA Kit; Protein related to DAN and cerberus ELISA Kit

Abbreviation

GREM2

Uniprot No.

Q9H772

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

6.25 pg/mL-2500 pg/mL

Sensitivity

6.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GREM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-100
1:2	Average %	95
	Range %	84-101
1:4	Average %	93
	Range %	87-100
1:8	Average %	90
	Range %	87-101

Recovery

The recovery of human GREM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-93
EDTA plasma (n=4)	95	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2500	0.155	0.149	0.152
469	0.342	0.352	0.347
78	0.559	0.569	0.564
22	1.001	0.963	0.982
6.25	1.543	1.510	1.527
0	2.184	2.086	2.135

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human GREM2 ELISA Kit was designed for the quantitative measurement of Human GREM2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 pg/mL-2500 pg/mL and the sensitivity is 6.25 pg/mL.

Target Background

Function
(From Uniprot)

Cytokine that inhibits the activity of BMP2 and BMP4 in a dose-dependent manner, and thereby modulates signaling by BMP family members. Contributes to the regulation of embryonic morphogenesis via BMP family members. Antagonizes BMP4-induced suppression of progesterone production in granulosa cells.

Gene References into Functions

TT genotype of the rs4454537 polymorphism protective against the development of osteoporosis PMID: 30014930
Our study confirmed that GREM2 is a candidate gene for tooth agenesis, which mutations can explain, however, only a small fraction of the genetic contribution to the pathogenesis of this anomaly. PMID: 28992378
Data show that the GREMLIN 2 (GREM2) expression during Induced Pluripotent Stem Cell (hiPS) cell cardiac differentiation follows the expression pattern of cardiac-specific genes. PMID: 28125926
The structure of Grem2-GDF5 complex has revealed a number of key findings for DAN-family mediated BMP2 inhibition. PMID: 27524626
This study showed that si-Grem2 increased the BMP-2-induced osteogenic differentiation of hBMSCs via the BMP-2/Smad/Runx2 pathway. PMID: 27335248
The present study shows that the Grem2 heparin/HS and BMP2-binding epitopes are unique and independent, where the Grem2-BMP2 complex exhibits a significant increase in binding affinity toward heparin moieties that appear to be partially independent of the Grem2 heparin/HS-binding epitope. PMID: 28104757
Gremlin 2 inhibits adipocyte differentiation through activation of Wnt/beta-catenin signaling PMID: 26239165
mutations associated with tooth malformations PMID: 26416033
GREM2 variants have been identified in atrial fibrillation cohort studies, demonstrating abnormalities in cardiac excitation - supra ventricular tachycardia and atrial fibrillation. PMID: 25443231
The minor allele of rs4454537 is significantly associated with low bone density at the total hip of southern Chinese people. Our study further suggests GREM2 as a novel susceptibility gene for osteoporosis. PMID: 23902946
Through regulation of bone morphogenetic protein signaling, GREM2 is required for cardiac laterality and atrial differentiation during embryonic development. PMID: 23223679
and that a genetic variant in the FMN2/GREM2 locus influences GREM2 expression in osteoblasts and thereby trabecular number and thickness as well as fracture risk. PMID: 23437003

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Involvement in disease

Tooth agenesis, selective, 9 (STHAG9)

Subcellular Location

Secreted.

Protein Families

DAN family

Database Links

HGNC: 17655

OMIM: 608832

KEGG: hsa:64388

STRING: 9606.ENSP00000318650

UniGene: Hs.98206

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Code	CSB-EL009893HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.