Recombinant Human Gremlin-2 (GREM2)

Code CSB-YP887993HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP887993HU
MSDS
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Source E.coli
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Code CSB-EP887993HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887993HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP887993HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GREM2
Uniprot No.
Alternative Names
BMP antagonist 2; CKTSF1B2; Cysteine knot superfamily 1; Cysteine knot superfamily 1 BMP antagonist 2; DAN domain family member 3; DAND 3; DAND3; GREM 2; Grem2; GREM2_HUMAN; Gremlin 2; Gremlin 2 cysteine knot superfamily homolog; Gremlin-2; Gremlin2; PRDC; Protein related to DAN and cerberus
Species
Homo sapiens (Human)
Expression Region
22-168
Target Protein Sequence
RKNRPAGAI PSPYKDGSSN NSERWQHQIK EVLASSQEAL VVTERKYLKS DWCKTQPLRQ TVSEEGCRSR TILNRFCYGQ CNSFYIPRHV KKEEESFQSC AFCKPQRVTS VLVELECPGL DPPFRLKKIQ KVKQCRCMSV NLSDSDKQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cytokine that inhibits the activity of BMP2 and BMP4 in a dose-dependent manner, and thereby modulates signaling by BMP family members. Contributes to the regulation of embryonic morphogenesis via BMP family members. Antagonizes BMP4-induced suppression of progesterone production in granulosa cells.
Gene References into Functions
  1. TT genotype of the rs4454537 polymorphism protective against the development of osteoporosis PMID: 30014930
  2. Our study confirmed that GREM2 is a candidate gene for tooth agenesis, which mutations can explain, however, only a small fraction of the genetic contribution to the pathogenesis of this anomaly. PMID: 28992378
  3. Data show that the GREMLIN 2 (GREM2) expression during Induced Pluripotent Stem Cell (hiPS) cell cardiac differentiation follows the expression pattern of cardiac-specific genes. PMID: 28125926
  4. The structure of Grem2-GDF5 complex has revealed a number of key findings for DAN-family mediated BMP2 inhibition. PMID: 27524626
  5. This study showed that si-Grem2 increased the BMP-2-induced osteogenic differentiation of hBMSCs via the BMP-2/Smad/Runx2 pathway. PMID: 27335248
  6. The present study shows that the Grem2 heparin/HS and BMP2-binding epitopes are unique and independent, where the Grem2-BMP2 complex exhibits a significant increase in binding affinity toward heparin moieties that appear to be partially independent of the Grem2 heparin/HS-binding epitope. PMID: 28104757
  7. Gremlin 2 inhibits adipocyte differentiation through activation of Wnt/beta-catenin signaling PMID: 26239165
  8. mutations associated with tooth malformations PMID: 26416033
  9. GREM2 variants have been identified in atrial fibrillation cohort studies, demonstrating abnormalities in cardiac excitation - supra ventricular tachycardia and atrial fibrillation. PMID: 25443231
  10. The minor allele of rs4454537 is significantly associated with low bone density at the total hip of southern Chinese people. Our study further suggests GREM2 as a novel susceptibility gene for osteoporosis. PMID: 23902946
  11. Through regulation of bone morphogenetic protein signaling, GREM2 is required for cardiac laterality and atrial differentiation during embryonic development. PMID: 23223679
  12. and that a genetic variant in the FMN2/GREM2 locus influences GREM2 expression in osteoblasts and thereby trabecular number and thickness as well as fracture risk. PMID: 23437003

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Involvement in disease
Tooth agenesis, selective, 9 (STHAG9)
Subcellular Location
Secreted.
Protein Families
DAN family
Database Links

HGNC: 17655

OMIM: 608832

KEGG: hsa:64388

STRING: 9606.ENSP00000318650

UniGene: Hs.98206

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