Human Growth/differentiation factor 11(GDF11) ELISA kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Name: Human Growth/differentiation factor 11(GDF11) ELISA kit
Brand: CUSABIO
SKU: CSB-EL009344HU

Product Details

Target Name

growth differentiation factor 11

Alternative Names

BMP 11 ELISA Kit; BMP-11 ELISA Kit; BMP11 ELISA Kit; Bone morphogenetic protein 11 ELISA Kit; GDF 11 ELISA Kit; GDF-11 ELISA Kit; Gdf11 ELISA Kit; GDF11_HUMAN ELISA Kit; Growth differentiation factor 11 ELISA Kit; Growth/differentiation factor 11 ELISA Kit

Abbreviation

GDF11

Uniprot No.

O95390

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

28 pg/mL-1800 pg/mL

Sensitivity

7 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GDF11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	88
	Range %	81-93
1:4	Average %	90
	Range %	85-96
1:8	Average %	97
	Range %	83-91

Recovery

The recovery of human GDF11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-98
EDTA plasma (n=4)	94	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1800	2.699	2.752	2.726	2.579
900	1.879	1.906	1.893	1.746
450	1.121	1.200	1.161	1.014
225	0.548	0.545	0.547	0.400
112.5	0.306	0.332	0.319	0.172
56	0.225	0.238	0.232	0.085
28	0.213	0.204	0.209	0.062
0	0.151	0.142	0.147

Materials provided

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human GDF11 This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled GDF11 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human GDF11 ELISA Kit was designed for the quantitative measurement of Human GDF11 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL .

Related Products

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

■ Q&A

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please provide your reference data for human plasma samples.
e. Did you study the cross-reactivity or interference between Growth Differentiation Factor 11 (GDF11) and analogues? If yes, please provide the corresponding data.

Thanks for your inquiry!
CSB-EL009344HU
Capture antibody is mouse monoclonal antibody and immunogen is CHO cell-derived recombinant protein，full length.
Detection antibody is goat polyclonal antibody and immunogen is CHO cell-derived recombinant protein, fragment. Standard is CHO cell-derived recombinant protein.
We tested undiluted normal serum and plasma samples before and the test value is ND--40pg/ml.
There is no cross-reactivity with human GDF15, human GDF5 and human GDF9.

Target Background

Function
(From Uniprot)

Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues. It is required for proper vertebral patterning and orofacial development. Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3.

Gene References into Functions

The serum content of GDF11 was much less in esophageal cancer patients than in the control group. Esophageal GDF II in cancer patients was correlated with cancer differentiation: the higher the degree of differentiation, the higher the content of GDF11. PMID: 30213293
Physical inactivity was significantly related to the decreased GDF11 levels in COPD. PMID: 29731621
GDF11 expression was decreased in COPD patients' serum and cells when compared with that of healthy people. PMID: 29680737
GDF11 may be a relevant myostatin-interacting peptide to successful aging in humans PMID: 28701523
The Growth Differentiation Factor 11 (GDF11) and Myostatin (MSTN) in tissue specific aging. PMID: 28472635
Tumor-suppressor inactivation of GDF11 occurs by precursor sequestration in triple-negative breast cancer PMID: 29161592
These studies identify distinctive structural features of GDF11 that enhance its potency, relative to GDF8; however, the biological consequences of these differences remain to be determined. PMID: 28257634
In elderly Chinese women, osteoporosis risk was significantly increased with increases in GDF11 serum levels. PMID: 27557752
A Prodomain Fragment from the Proteolytic Activation of Growth Differentiation Factor 11 Remains Associated with the Mature Growth Factor and Keeps It Soluble PMID: 28715204
MSTN, but not GDF11, declines in healthy men throughout aging. PMID: 27304512
GDF11 is highly concentrated in human platelets. PMID: 27509407
The crystal structure of GDF11 was determined to a resolution of 1.50 A. PMID: 26919518
GDF11 is essential for mammalian development and has been suggested to regulate aging of multiple tissues. It functions in the heart, skeletal muscle, and brain. Review. PMID: 27034275
GDF11 inhibits rather than helps muscle regeneration. PMID: 26001423
Show that there is no age-related cardiac hypertrophy in disease-free 24-month-old C57BL/6 mice and that restoring GDF11 in old mice has no effect on cardiac structure or function. PMID: 26383970
in vitro sprout formation was increased as well by GDF11 treatment PMID: 26026854
Suggest GDF11 functions as encephalic regionalizing factor in neural differentiated mouse embryonic stem cells. PMID: 25352416
GDF11 is a critical rheostat for bone turnover and a key integrator of bone homeostasis. PMID: 25534870
These data demonstrate GDF11 to be a master regulator of neural stem cell transcription that can suppress cell proliferation and migration by regulating the expression of numerous genes involved in both these processes PMID: 24244313
Expression of GDF11, a cytokine which blocks terminal erythroid maturation, was increased in erthyroblasts of thalassemic patients. PMID: 24658077
Quantitative real-time reverse transcription-PCR in colorectal cancer specimens obtained from 130 patients showed that GDF11 mRNA expression in cancer tissue was significantly higher than in normal tissue PMID: 17912435
Members of the transforming growth factor beta (TGFbeta) superfamily, bone morphogenetic protein 2 (BMP2), and growth and differentiation factor 11 (GDF11), can signal cultured RGCs to form dendrites. PMID: 17997109
We propose that Pcsk5, at least in part via GDF11, coordinately regulates caudal Hox paralogs, to control anteroposterior patterning, nephrogenesis, skeletal, and anorectal development. PMID: 18519639
Differential antagonism of activin, myostatin and growth and differentiation factor 11 by wild-type and mutant follistatin. PMID: 18535106
Both WFIKKN1 and WFIKKN2 have high affinity for growth and differentiation factors 8 and 11. PMID: 18596030
Myostatin or 20 ng/mL BMP-11 maintain the colony and cellular morphology of undifferentiated hESC, maintain POU5f1, NANOG, TRA-1-60, and SSEA4 expression, and display increased SMAD2/3 phosphorylation PMID: 19751112

Hide All

Subcellular Location

Secreted.

Protein Families

TGF-beta family

Tissue Specificity

In the embryo, strong expression is seen in the palatal epithelia, including the medial edge epithelial and midline epithelial seam of the palatal shelves. Less pronounced expression is also seen throughout the palatal shelf and tongue mesenchyme.

Database Links

HGNC: 4216

OMIM: 603936

KEGG: hsa:10220

STRING: 9606.ENSP00000257868

UniGene: Hs.600883

Code	CSB-EL009344HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.