Human Insulin-like growth factor 2 mRNA-binding protein 3(IGF2BP3) ELISA kit

Code CSB-EL011092HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O00425
Lead Time 7-14 working days
Abbreviation IGF2BP3
Target Name insulin-like growth factor 2 mRNA binding protein 3
Alias CT98, DKFZp686F1078, IMP-3, IMP3, KOC1, VICKZ3, IGF II mRNA binding protein 3|IGF-II mRNA-binding protein 3|KH domain containing protein overexpressed in cancer|cancer/testis antigen 98
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 37.5 pg/mL-2400 pg/mL
Sensitivity 9.37 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is primarily found in the nucleolus, where it can bind to the 5 UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.
HGNC 28868
RGD 1306512
MGI 1890359
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IGF2BP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %86-95
1:2Average %102
Range %97-107
1:4Average %91
Range %85-97
1:8Average %97
Range %91-103
Recovery
The recovery of human IGF2BP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-96
EDTA plasma (n=4)9793-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
24002.784 2.799 2.792 2.640
12001.956 1.906 1.931 1.779
6001.095 1.123 1.109 0.957
3000.637 0.665 0.651 0.499
1500.419 0.451 0.435 0.283
750.338 0.342 0.340 0.188
37.50.247 0.268 0.258 0.106
00.151 0.153 0.152
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Function RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
Subcellular Location Nucleus, Cytoplasm
Protein Families RRM IMP/VICKZ family
Tissue Specificity Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic
Database Links

HGNC: 28868

OMIM: 608259

KEGG: hsa:10643

STRING: 9606.ENSP00000258729

UniGene: Hs.700696

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