Human Insulin-like growth factor-binding protein 7 (IGFBP7/MAC25/PSF) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

insulin-like growth factor binding protein 7

Associated names

AGM ELISA Kit; Angiomodulin ELISA Kit; FSTL2 ELISA Kit; IBP-7 ELISA Kit; IBP7 ELISA Kit; IBP7_HUMAN ELISA Kit; IGF binding protein 7 ELISA Kit; IGF-binding protein 7 ELISA Kit; IGFBP rP1 ELISA Kit; IGFBP-7 ELISA Kit; IGFBP-rP1 ELISA Kit; IGFBP7 ELISA Kit; IGFBPRP1 ELISA Kit; Insulin like growth factor binding protein 7 ELISA Kit; Insulin-like growth factor-binding protein 7 ELISA Kit; MAC25 ELISA Kit; MAC25 protein ELISA Kit; PGI2 stimulating factor ELISA Kit; PGI2-stimulating factor ELISA Kit; Prostacyclin stimulating factor ELISA Kit; Prostacyclin-stimulating factor ELISA Kit; PSF ELISA Kit; RAMSVPS ELISA Kit; TAF ELISA Kit; Tumor-derived adhesion factor ELISA Kit

Abbreviation

IGFBP7

Uniprot No.

Q16270

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

31.25pg/mL-2000pg/mL

Sensitivity

15.6pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP7/MAC25/PSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	87
	Range %	83-92
1:200	Average %	92
	Range %	85-99
1:400	Average %	101
	Range %	95-108
1:800	Average %	93
	Range %	86-99

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	3.038	2.934	2.986	2.818
1000	2.334	2.197	2.265	2.098
500	1.625	1.555	1.590	1.422
250	1.040	1.101	1.070	0.903
125	0.616	0.621	0.619	0.451
62.5	0.443	0.453	0.448	0.281
31.25	0.271	0.280	0.275	0.108
0	0.170	0.165	0.167

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

Description

This ELISA kit provides quantitative measurement of IGFBP7 (insulin-like growth factor binding protein 7) in human samples, supporting research into its roles in cellular senescence, TGF-β signaling, and tumor suppression. Because IGFBP7 functions as both a secreted autocrine/paracrine factor and a circulating biomarker with concentrations that shift markedly between healthy and disease states, researchers need an assay that accommodates diverse sample contexts without extensive optimization.

Detection Range: The 31.25–2000 pg/mL range spans the concentrations typically observed in human serum and plasma, where circulating IGFBP7 levels can rise several-fold in renal injury, heart failure, and advanced malignancies. Most serum samples require minimal or no dilution, simplifying assay planning.

Sensitivity: At 15.6 pg/mL, the kit detects IGFBP7 in low-abundance conditions such as conditioned media from senescence-resistant cell lines or early-stage kidney injury samples where secreted levels are just emerging above baseline.

Sample Compatibility: Validation across serum, plasma, cell culture supernates, and tissue homogenates enables researchers to correlate circulating IGFBP7 with local tissue expression and in vitro secretion within a single study, critical for dissecting its paracrine versus endocrine functions.

Assay Efficiency: Requiring only 50–100 µL per well and 1–5 hours to complete, the kit accommodates limited clinical specimens and primary cell-conditioned media while supporting same-day results.

This kit is particularly suited for studies in acute kidney injury biomarker discovery, oncogene-induced senescence, and cardiac fibrosis research.

Code	CSB-E17249h-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.