Product Details

Uniprot No.

Target Names

Alternative Names

Insulin-like growth factor-binding protein 7 (IBP-7) (IGF-binding protein 7) (IGFBP-7) (IGFBP-rP1) (MAC25 protein) (PGI2-stimulating factor) (Prostacyclin-stimulating factor) (Tumor-derived adhesion factor) (TAF), IGFBP7, MAC25 PSF

Species Reactivity

Human

Immunogen

A synthesized peptide from human IGFBP7 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5E10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

IGFBP7, also known as insulin-like growth factor-binding protein 7 or MAC25, plays a multifaceted role in cellular regulation beyond its classical function of modulating IGF signaling. This secreted protein influences cellular senescence, apoptosis, and adhesion processes, making it a compelling target in cancer biology, vascular research, and studies of metabolic disease. Its documented involvement in tumor suppression and angiogenesis has positioned IGFBP7 as a biomarker of interest across multiple disease contexts.

This recombinant rabbit monoclonal antibody, clone 5E10, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human IGFBP7, the antibody undergoes affinity chromatography purification to ensure reliable performance across experiments and extended studies.

Validation data demonstrates robust detection in Western blot applications across multiple human cell lines, including Jurkat, HeLa, HepG2, and THP-1 lysates. The observed band at approximately 34 kDa, compared to the predicted 29 kDa molecular weight, likely reflects post-translational modifications such as glycosylation, which is characteristic of this secreted protein. Immunohistochemistry validation in paraffin-embedded human endometrial carcinoma tissue confirms utility for pathological investigations, while immunofluorescence staining in HeLa cells reveals cytoplasmic localization patterns. Flow cytometry analysis using 786-O renal carcinoma cells demonstrates clear positive signal separation from isotype controls, supporting quantitative single-cell studies.

With validated performance spanning Western blot, immunohistochemistry, immunofluorescence, and flow cytometry, this antibody provides flexibility for researchers investigating IGFBP7 in oncology, senescence biology, and metabolic regulation contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA099607A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg), Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes: IBP 7 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time：3min IHC image of CSB-RA099607A0HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer/endometrial carcinoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA099607A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing 786-O cells stained with CSB-RA099607A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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IGFBP7 Recombinant Monoclonal Antibody

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