Human Inter-alpha-trypsin inhibitor heavy chain H4(ITIH4/IHRP/ITIHL1/PK120/PRO1851)ELISA kit

Code CSB-E17022h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
Alternative Names
35 kDa inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; gp120 ELISA Kit; H4P ELISA Kit; IHRP ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; inter-alpha-trypsin inhibitor, heavy chain-like, 1 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; ITIH4 ELISA Kit; ITIH4_HUMAN ELISA Kit; ITIHL1 ELISA Kit; OTTHUMP00000197120 ELISA Kit; OTTHUMP00000197121 ELISA Kit; OTTHUMP00000213834 ELISA Kit; OTTHUMP00000213869 ELISA Kit; PK-120 ELISA Kit; PK120 ELISA Kit; Plasma kallikrein sensitive glycoprotein 120 ELISA Kit; PRO1851 ELISA Kit
Abbreviation
ITIH4
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 82-97  
1:2 Average % 97  
Range % 92-102  
1:4 Average % 96  
Range % 90-104  
1:8 Average % 97  
Range % 92-102  
Recovery
The recovery of human ITIH4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 106 98-110  
EDTA plasma (n=4) 95 91-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.539 2.438 2.489 2.329  
20 2.047 1.946 1.997 1.837  
10 1.312 1.301 1.307 1.147  
5 0.755 0.766 0.761 0.601  
2.5 0.463 0.452 0.458 0.298  
1.25 0.337 0.326 0.332 0.172  
0.625 0.242 0.245 0.244 0.084  
0 0.160 0.159 0.160    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human ITIH4 ELISA Kit was designed for the quantitative measurement of Human ITIH4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

Function
(From Uniprot)
Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
Gene References into Functions
  1. Low ITIH4 expression is associated with Hepatocellular Carcinoma. PMID: 28828637
  2. this report has further supported for associations of genetic variants in the ITIH4 and CALN1 genes with schizophrenia and provided the first evidence that the variants regulate ITIH4 AND CALN1 expression in the dorsolateral prefrontal cortex PMID: 26991396
  3. ITIH4 peptide isoform as a preterm birth biomarker and its associated SNP implications PMID: 26408095
  4. Serum ITIH4 may be a PM10-specific biomarker in COPD and may be related to inflammation. PMID: 25977605
  5. Four novel body mass index-associated loci near the KCNQ1(rs2237892), ALDH2/MYL2 (rs671, rs12229654), ITIH4 (rs2535633) and NT5C2 (rs11191580) genes are identified in East Asian-ancestry populations. PMID: 24861553
  6. Worse survival among HCC patients with low ITIH4. PMID: 24836184
  7. Isoform-specific ITIH4 glycosylation and utilization of O-glycosylation sites on ITIH4 differs between cell lines and serum. PMID: 24884609
  8. Expression of the 85 kDa ITIH4 was substantial in amyotrophic lateral sclerosis compared with controls or patients with muscular dystrophy, Alzheimer diseases, or Parkinson diseases PMID: 23436019
  9. A truncated fragment of inter-alpha-trypsin inhibitor heavy chain 4 was the sole protein found to be significantly enhanced in the prostate cancer patients compared to the controls. PMID: 23417432
  10. Findings suggest that ITI-H4 expression may be used as a biomarker, which could facilitate the development of novel diagnostic and therapeutic tools. PMID: 21331437
  11. Genetic variation at ITIH4 locus is one of the likely candidate determinants for plasma cholesterol metabolisms PMID: 14661079
  12. ITIH4 and MTJ1 co-immunoprecipitate from total liver protein extracts and SANT domain of HTJ1 protects the ITIH4(588-930) recombinant fragment PMID: 16271702
  13. The inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4) protein was significantly present more in interstial cystitis than controls PMID: 18455532
  14. ITIH4 is an anti-inflammatory protein, and suggests that further investigation into its potential use in the diagnosis and prognosis of acute ischemic stroke is warranted. PMID: 19263524

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Subcellular Location
Secreted.
Protein Families
ITIH family
Tissue Specificity
Liver specific.
Database Links

HGNC: 6169

OMIM: 600564

KEGG: hsa:3700

STRING: 9606.ENSP00000266041

UniGene: Hs.709406

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