Human Interferon alpha-21(IFNA21) ELISA kit

Instructions
Code CSB-EL011039HU
Size 96T,5×96T,10×96T

Product Details

Target Name interferon, alpha 21
Alternative Names MGC126687, MGC126689, leukocyte interferon protein
Abbreviation IFNA21
Uniprot No. P01568
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IFNA21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %91-101
1:2Average %104
Range %98-107
1:4Average %87
Range %83-91
1:8Average %94
Range %90-97
Recovery
The recovery of human IFNA21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9184-94
EDTA plasma (n=4)10196-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.749 2.701 2.725 2.576
5001.752 1.786 1.769 1.620
2501.177 1.124 1.151 1.002
1250.729 0.712 0.721 0.572
62.50.509 0.487 0.498 0.349
31.20.350 0.343 0.347 0.198
15.60.256 0.249 0.253 0.104
00.148 0.150 0.149
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes
Subcellular Location Secreted
Protein Families Alpha/beta interferon family
Database Links

HGNC: 5424

OMIM: 147584

KEGG: hsa:3452

STRING: 9606.ENSP00000369574

UniGene: Hs.113211

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