Human Interleukin 18,IL-18 ELISA KIT

Code CSB-E07450h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
20% off above 2 kits (96T) per item
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
interleukin 18 (interferon-gamma-inducing factor)
Alternative Names
Iboctadekin ELISA Kit; IFN gamma inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; IGIF ELISA Kit; IL 1 gamma ELISA Kit; IL 18 ELISA Kit; IL 1g ELISA Kit; IL-1 gamma ELISA Kit; IL-18 ELISA Kit; IL1 gamma ELISA Kit; IL18 ELISA Kit; IL18 protein ELISA Kit; IL18_HUMAN ELISA Kit; IL1F4 ELISA Kit; IL1g ELISA Kit; IL1gamma ELISA Kit; ILIF4 ELISA Kit; Interferon gamma inducing factor ELISA Kit; Interferon gamma-inducing factor ELISA Kit; Interleukin 1 gamma ELISA Kit; Interleukin 18 (interferon-gamma-inducing factor) ELISA Kit; Interleukin 18 ELISA Kit; Interleukin-1 gamma ELISA Kit; Interleukin-18 ELISA Kit; Interleukin18 ELISA Kit; MGC12320 ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, urine, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
7.8 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human IL-18 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • Biotin-labeledIL-18 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
  • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
  • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
  • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Squirt bottle, manifold dispenser, or automated microplate washer.
  • Absorbent paper for blotting the microtiter plate.
  • 100ml and 500ml graduated cylinders.
  • Deionized or distilled water.
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips.
  • Timer
  • Test tubes for dilution.
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human IL18 ELISA Kit was designed for the quantitative measurement of Human IL18 protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .


Customer Reviews and Q&A

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I have a question about your ELISA Kits for IL18. What are the difference between IL18 binding protein, IL18 receptor 1 and the IL 18 kit? I am working on the earlier stage of renal failure on diabetic patient. So I would like to know which Protein will be more sensitive and efficient for my research.

Thanks for your inquiry.
IL18,IL18 binding protein, IL18 receptor 1 are in the IL18 family but they are different proteins.We checked some document related to the renal failure, there is much research on IL18 and IL18BP and you can consider this 2 proteins.
However, the occurrence of each disease is the result of multi-factor regulation. We suggest you check much more references and choose the proper one.

I am planning to carry out a study to assess level of Interleukin from Gingival Crevicular Fluid in Chronic Periodontitis patients before and after treatment.
Kindly provide the ELISA kit for analysis of Interleukin IL- 18. How many sample can be analysed by each kit ?

Thanks for your inquiry.
#CSB-E07450h,Human Interleukin 18,IL-18 ELISA KIT
We have them all available.They are all appliedwith sandwich enzyme immunoassay technique.For a 96T sandwich ELISA kit (doubleantibody), we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for
the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.
If you wan to test Gingival Crevicular Fluid ,we suggest you do pre-testing first.

Target Background

(From Uniprot)
A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (Probable). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (Probable).
Gene References into Functions
  1. The results of the linkage disequilibrium test showed that the -137G/C and -607G/T sites had no linkage disequilibrium in the present study. PMID: 29926984
  2. SNPs associated with genetically determined high activity of TLR5 among patients with CD and genetically determined high IL-12 and IL-18 levels among patients with UC. PMID: 28139755
  3. The adipose tissue IL-18R/IL-18 expression is enhanced in obesity which associates with proinflammatory gene signature and insulin resistance in these individuals. PMID: 28508444
  4. Our study implied that elevated IL-18 expression and downregulation of GAS5 in serums might contribute to insulin resistance in polycystic ovary syndrome patients. PMID: 29648472
  5. Studied serum level of interleukin 18 (IL18) in HIV and Hepatitis C (HCV) coinfection and monoinfection. Found shown that IL-18 is increased in HIV/HCV coinfection when compared with monoinfection and that this increase is likely due to additive sensing of both viruses. PMID: 27927859
  6. not associated with obesity in this study PMID: 28634744
  7. multiple myeloma patients with IL-18 (rs16944) TT had the highest hemoglobin concentration PMID: 30211233
  8. These results suggest that increased IL-18 secretion from larger numbers of neutrophils elicits mechanical hyperalgesia. PMID: 29353540
  9. The results of this study suggests that IL-18 rs187238 and rs1946518 SNPs may be the cause of the AA susceptibility. PMID: 29349811
  10. Studied association of single nucleotide polymorphisms of interleukin-18 (IL 18) and lower extremity deep venous thrombosis in a Chinese population. PMID: 29105174
  11. PI3K is a signal linker between L-selectin and PSGL-1 in IL-18 transcriptional activation at the promoter level. PMID: 29218606
  12. IL-18 is a susceptibility gene for the disease in southern Chinese children. PMID: 30059753
  13. Postoperative serum Cystatin C, IL-18 and Uric acid after 24h were significantly associated with acute kidney injury PMID: 29627485
  14. IL-18 is elevated in polycystic ovary syndrome patients, even in lean ones, and is correlated with insulin resistance and atherosclerotic cardiovascular risk PMID: 29105530
  15. HuR plays an important role in the progression of esophageal carcinoma by targeting IL-18. PMID: 28231690
  16. The IL18 promoter SNP, rs1946518, is a potential risk factor of periodontitis among young Japanese women. PMID: 29129846
  17. The results of this study suggest that IL18 rs187238 and rs1946518 polymorphisms may be associated with an increased risk of gestational diabetes mellitus as well as with BMI in pregnant women. PMID: 28580570
  18. This is the first study to demonstrate that higher pre-treatment IL-18 is associated with HBeAg seroconversion in patients with HIV/HBV co-infection after 48 weeks of HBV cART, which suggests a role for the inflammasome in a favorable response to treatment. PMID: 28195558
  19. Polymorphisms in the IL-18 gene are associated with viral load in Hepatitis B virus . PMID: 30045250
  20. higher level of IL-18 was observed in the monocyte supernatant of the extremely preterm group during late-onset sepsis PMID: 29042061
  21. Increased production of IL-18 in the gut-associated lymphoid tissue and peripheral blood of HIV infected patients. PMID: 29672590
  22. this review discusses IL-18 biological properties and role in disease pathogenesis PMID: 29247988
  23. IL-18 mRNA expression was increased significantly in chronic myeloid leukemia patients PMID: 29097263
  24. these results suggest that the HIV-induced IL-18 plays a role in increased intestinal permeability and microbial translocation observed in HIV-infected individuals. PMID: 29601578
  25. IL-18 gene variants may serve as important biomarkers in predicting the occurrence of nasopharyngeal carcinoma in Asian population. PMID: 29185028
  26. The association of polymorphisms -137 (rs187238) and -607 (rs1946518) in the interleukin-18 (IL-18) promoter with risk of hepatocellular carcinoma (HCC). PMID: 28000712
  27. These findings indicate that the IL-18 rs1946518 SNP in the donor liver is a risk factor for developing bacterial infection after liver transplantation. PMID: 29061223
  28. Serum levels of IL-18 in nephropathic patient significantly rise and Serum levels of IL-18 might be a predictor factor of progression of diabetic nephropathy. PMID: 27663212
  29. A higher IL-18 immunostaining in stroma of IBC and LABC was observed in comparison with tumor cells, but stromal immunoexpression was similar between IBC and LABC. PMID: 28766916
  30. IL-18 was increased in lung myeloid cells in severe chronic obstructive pulmonary disease. PMID: 28830544
  31. a large proportion blood basophils from patients with asthma express IL-18 and IL-18BP. mast cells and basophils are implicated in the pathogenesis of asthma via an IL-18-related mechanism. PMID: 29505668
  32. On PICU day 1, interleukin-18 predicted acute kidney injury with area under the curve=0.82, but neutrophil gelatinase-associated lipocalin and liver fatty acid binding protein predicted acute kidney injury with area under the curve of less than or equal to 0.69; on PICU day 2, area under the curve was higher. Interleukin-18 and liver fatty acid binding protein on day 1 predicted prolonged acute kidney injury. PMID: 28430754
  33. Data show that tumor-derived IL-18 induced PD-1 expression on NK cells and is associated with poor prognosis in triple-negative breast cancer. PMID: 28415798
  34. IL-23, alone and in combination with IL-18 and sRAGE, identified bacterial meningitis with excellent accuracy. Following validation, these markers could aid clinicians in diagnosis of bacterial meningitis and decision-making regarding prolongation of antibiotic therapy PMID: 29394248
  35. cardiac IL-18 and circulating IL-18 are involved in the pathogenesis of Fabry cardiomyopathy and left ventricular hypertrophy. PMID: 27888626
  36. Erdr1 is negatively expressed relative to Il-18 in psoriatic lesional skin. PMID: 26990875
  37. rs187238 was not associated with T1D in the Euro-Brazilian population. PMID: 28973736
  38. IL-18 (mRNA) in skeletal muscle appears to be involved in the regulation of intramuscular lipid metabolism and hypertriglyceridemia PMID: 29342149
  39. The evidence for influenza A virus activation via an indirect, IL-18-dependent mechanism indicates that MAIT cells are protective in influenza, and also possibly in any human disease process in which inflammation and IL-18 production occur. PMID: 27543331
  40. The development of urticaria, asthma, dermatitis, rhinitis, and eosinophilic disorders all have demonstrated correlations to increased IL-18 levels either in the tissue or systemically. IL-18 represents a novel site of immune regulation in not only allergic conditions, but also autoimmune diseases and other instances of aberrant immune functioning. PMID: 27496752
  41. Results suggest that a common functional IL18 haplotype associated with heightened proinflammatory responses confers susceptibility to stress-related depression and anxiety through effects on threat-related amygdala function, a risk pathway specific to women. PMID: 27430614
  42. human platelets contain transcripts for the IL-18 gene. They synthesize the cytokine de novo, process and release it upon activation. PMID: 27914933
  43. abnormal IL-18 expression is induced by genital infection and induces damage to male reproductive capacity, thereby causing male infertility PMID: 26856767
  44. IL-18 level was found to be significantly elevated in CAD patients compared with control individuals PMID: 28059484
  45. The serum levels of IL-37, which were correlated with antibody production and the serum levels of total IL-18 and IL-18BP, were elevated in the patients with primary Sjogren's syndrome. PMID: 28057714
  46. an imbalance in the production of IL-18 and its antagonist (an increase in the production of IL-18 with a decrease, no increase or an insufficient increase in the production of IL-18BP) has been described in many chronic inflammatory diseases in humans. PMID: 26898120
  47. among systemic sclerosis patients in China, dyspnea was significantly associated with IL-18 -607C/A genotype frequency PMID: 27098064
  48. We conclude that IL-18 protein and messenger RNA levels are increased in the endometrium of patients with PCOS, and this effect is correlated with body mass index. PMID: 27313119
  49. Serum IL-18 levels are significantly elevated in depressive patients. PMID: 27455146
  50. IL-18 cooperates with IL-15 to promote group 3 innate lymphoid cell (ILC3) proliferation and IL-22 production; describe an IL-18-induced, NF-kappaB-mediated mechanism that regulates IL-22 in ILC3s; at steady-state, IL-18 produced by dendritic cells mediates IL-22 production by ILC3s to help maintain normal tissue integrity PMID: 28842466

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Subcellular Location
Cytoplasm. Secreted.
Protein Families
IL-1 family
Database Links

HGNC: 5986

OMIM: 600953

KEGG: hsa:3606

STRING: 9606.ENSP00000280357

UniGene: Hs.83077

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