Recombinant Human Interleukin-18(IL18)

In Stock
Code CSB-YP614514HU(A4)
Size US$1298Purchase it in Cusabio online store
(only available for customers from the US)
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details


The recombinant human IL18 protein is encoded by a recombinant DNA that was cloned into the expression vector and then transfected into the yeast cells that support the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 10xHis tag gene to the gene fragment coding for the 1-193aa of the human IL18 protein. After purification, the product is the recombinant human IL18 protein. This recombinant IL18 protein was subjected to the SDS-PAGE determination. Its purity reaches over 90% evaluated by Bandscan software analysis combined with SAS-PAGE. This IL18 protein ran to the molecular weight of about 25 kDa under SDS-PAGE condition. It may find use in immunology research.

Interleukin-18 (IL-18) belongs to the IL-1 family of cytokines. The inactive IL-18 precursor is synthesized as its active form by caspase-1. In humans, increased disease severity can be associated with an imbalance of IL-18. IL-18 has been found to be associated with the injury of different organs. Furthermore, the overly imbalance of cytokines could cause potentially fatal conditions such as a cytokine storm. IL-18 forms a signaling complex by binding to the IL-18 alpha chain. It has been reported that IL-18 is associated with hyperphagia and a variety of metabolic syndrome. Importantly, increasing numbers of animal and clinical studies indicate a role for IL-18 in heart disease.

Purity Greater than 90% as determined by SDS-PAGE.
Target Names IL18
Uniprot No. Q14116
Research Area Immunology
Alternative Names Iboctadekin; IFN gamma inducing factor; IFN-gamma-inducing factor; IGIF; IL 1 gamma; IL 18; IL 1g; IL-1 gamma; IL-18; IL1 gamma; IL18; IL18 protein; IL18_HUMAN; IL1F4; IL1g; IL1gamma; ILIF4; Interferon gamma inducing factor; Interferon gamma-inducing factor; Interleukin 1 gamma; Interleukin 18 (interferon-gamma-inducing factor); Interleukin 18; Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320
Species Homo sapiens (Human)
Source Yeast
Expression Region 1-193aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 24.8kDa
Protein Length Full?Length
Tag Info N-terminal 10xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (Probable). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (Probable).
Gene References into Functions
  1. The results of the linkage disequilibrium test showed that the -137G/C and -607G/T sites had no linkage disequilibrium in the present study. PMID: 29926984
  2. SNPs associated with genetically determined high activity of TLR5 among patients with CD and genetically determined high IL-12 and IL-18 levels among patients with UC. PMID: 28139755
  3. The adipose tissue IL-18R/IL-18 expression is enhanced in obesity which associates with proinflammatory gene signature and insulin resistance in these individuals. PMID: 28508444
  4. Our study implied that elevated IL-18 expression and downregulation of GAS5 in serums might contribute to insulin resistance in polycystic ovary syndrome patients. PMID: 29648472
  5. Studied serum level of interleukin 18 (IL18) in HIV and Hepatitis C (HCV) coinfection and monoinfection. Found shown that IL-18 is increased in HIV/HCV coinfection when compared with monoinfection and that this increase is likely due to additive sensing of both viruses. PMID: 27927859
  6. not associated with obesity in this study PMID: 28634744
  7. multiple myeloma patients with IL-18 (rs16944) TT had the highest hemoglobin concentration PMID: 30211233
  8. These results suggest that increased IL-18 secretion from larger numbers of neutrophils elicits mechanical hyperalgesia. PMID: 29353540
  9. The results of this study suggests that IL-18 rs187238 and rs1946518 SNPs may be the cause of the AA susceptibility. PMID: 29349811
  10. Studied association of single nucleotide polymorphisms of interleukin-18 (IL 18) and lower extremity deep venous thrombosis in a Chinese population. PMID: 29105174
  11. PI3K is a signal linker between L-selectin and PSGL-1 in IL-18 transcriptional activation at the promoter level. PMID: 29218606
  12. IL-18 is a susceptibility gene for the disease in southern Chinese children. PMID: 30059753
  13. Postoperative serum Cystatin C, IL-18 and Uric acid after 24h were significantly associated with acute kidney injury PMID: 29627485
  14. IL-18 is elevated in polycystic ovary syndrome patients, even in lean ones, and is correlated with insulin resistance and atherosclerotic cardiovascular risk PMID: 29105530
  15. HuR plays an important role in the progression of esophageal carcinoma by targeting IL-18. PMID: 28231690
  16. The IL18 promoter SNP, rs1946518, is a potential risk factor of periodontitis among young Japanese women. PMID: 29129846
  17. The results of this study suggest that IL18 rs187238 and rs1946518 polymorphisms may be associated with an increased risk of gestational diabetes mellitus as well as with BMI in pregnant women. PMID: 28580570
  18. This is the first study to demonstrate that higher pre-treatment IL-18 is associated with HBeAg seroconversion in patients with HIV/HBV co-infection after 48 weeks of HBV cART, which suggests a role for the inflammasome in a favorable response to treatment. PMID: 28195558
  19. Polymorphisms in the IL-18 gene are associated with viral load in Hepatitis B virus . PMID: 30045250
  20. higher level of IL-18 was observed in the monocyte supernatant of the extremely preterm group during late-onset sepsis PMID: 29042061
  21. Increased production of IL-18 in the gut-associated lymphoid tissue and peripheral blood of HIV infected patients. PMID: 29672590
  22. this review discusses IL-18 biological properties and role in disease pathogenesis PMID: 29247988
  23. IL-18 mRNA expression was increased significantly in chronic myeloid leukemia patients PMID: 29097263
  24. these results suggest that the HIV-induced IL-18 plays a role in increased intestinal permeability and microbial translocation observed in HIV-infected individuals. PMID: 29601578
  25. IL-18 gene variants may serve as important biomarkers in predicting the occurrence of nasopharyngeal carcinoma in Asian population. PMID: 29185028
  26. The association of polymorphisms -137 (rs187238) and -607 (rs1946518) in the interleukin-18 (IL-18) promoter with risk of hepatocellular carcinoma (HCC). PMID: 28000712
  27. These findings indicate that the IL-18 rs1946518 SNP in the donor liver is a risk factor for developing bacterial infection after liver transplantation. PMID: 29061223
  28. Serum levels of IL-18 in nephropathic patient significantly rise and Serum levels of IL-18 might be a predictor factor of progression of diabetic nephropathy. PMID: 27663212
  29. A higher IL-18 immunostaining in stroma of IBC and LABC was observed in comparison with tumor cells, but stromal immunoexpression was similar between IBC and LABC. PMID: 28766916
  30. IL-18 was increased in lung myeloid cells in severe chronic obstructive pulmonary disease. PMID: 28830544
  31. a large proportion blood basophils from patients with asthma express IL-18 and IL-18BP. mast cells and basophils are implicated in the pathogenesis of asthma via an IL-18-related mechanism. PMID: 29505668
  32. On PICU day 1, interleukin-18 predicted acute kidney injury with area under the curve=0.82, but neutrophil gelatinase-associated lipocalin and liver fatty acid binding protein predicted acute kidney injury with area under the curve of less than or equal to 0.69; on PICU day 2, area under the curve was higher. Interleukin-18 and liver fatty acid binding protein on day 1 predicted prolonged acute kidney injury. PMID: 28430754
  33. Data show that tumor-derived IL-18 induced PD-1 expression on NK cells and is associated with poor prognosis in triple-negative breast cancer. PMID: 28415798
  34. IL-23, alone and in combination with IL-18 and sRAGE, identified bacterial meningitis with excellent accuracy. Following validation, these markers could aid clinicians in diagnosis of bacterial meningitis and decision-making regarding prolongation of antibiotic therapy PMID: 29394248
  35. cardiac IL-18 and circulating IL-18 are involved in the pathogenesis of Fabry cardiomyopathy and left ventricular hypertrophy. PMID: 27888626
  36. Erdr1 is negatively expressed relative to Il-18 in psoriatic lesional skin. PMID: 26990875
  37. rs187238 was not associated with T1D in the Euro-Brazilian population. PMID: 28973736
  38. IL-18 (mRNA) in skeletal muscle appears to be involved in the regulation of intramuscular lipid metabolism and hypertriglyceridemia PMID: 29342149
  39. The evidence for influenza A virus activation via an indirect, IL-18-dependent mechanism indicates that MAIT cells are protective in influenza, and also possibly in any human disease process in which inflammation and IL-18 production occur. PMID: 27543331
  40. The development of urticaria, asthma, dermatitis, rhinitis, and eosinophilic disorders all have demonstrated correlations to increased IL-18 levels either in the tissue or systemically. IL-18 represents a novel site of immune regulation in not only allergic conditions, but also autoimmune diseases and other instances of aberrant immune functioning. PMID: 27496752
  41. Results suggest that a common functional IL18 haplotype associated with heightened proinflammatory responses confers susceptibility to stress-related depression and anxiety through effects on threat-related amygdala function, a risk pathway specific to women. PMID: 27430614
  42. human platelets contain transcripts for the IL-18 gene. They synthesize the cytokine de novo, process and release it upon activation. PMID: 27914933
  43. abnormal IL-18 expression is induced by genital infection and induces damage to male reproductive capacity, thereby causing male infertility PMID: 26856767
  44. IL-18 level was found to be significantly elevated in CAD patients compared with control individuals PMID: 28059484
  45. The serum levels of IL-37, which were correlated with antibody production and the serum levels of total IL-18 and IL-18BP, were elevated in the patients with primary Sjogren's syndrome. PMID: 28057714
  46. an imbalance in the production of IL-18 and its antagonist (an increase in the production of IL-18 with a decrease, no increase or an insufficient increase in the production of IL-18BP) has been described in many chronic inflammatory diseases in humans. PMID: 26898120
  47. among systemic sclerosis patients in China, dyspnea was significantly associated with IL-18 -607C/A genotype frequency PMID: 27098064
  48. We conclude that IL-18 protein and messenger RNA levels are increased in the endometrium of patients with PCOS, and this effect is correlated with body mass index. PMID: 27313119
  49. Serum IL-18 levels are significantly elevated in depressive patients. PMID: 27455146
  50. IL-18 cooperates with IL-15 to promote group 3 innate lymphoid cell (ILC3) proliferation and IL-22 production; describe an IL-18-induced, NF-kappaB-mediated mechanism that regulates IL-22 in ILC3s; at steady-state, IL-18 produced by dendritic cells mediates IL-22 production by ILC3s to help maintain normal tissue integrity PMID: 28842466

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Subcellular Location Cytoplasm. Secreted.
Protein Families IL-1 family
Database Links

HGNC: 5986

OMIM: 600953

KEGG: hsa:3606

STRING: 9606.ENSP00000280357

UniGene: Hs.83077


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