Human Interleukin-27 subunit beta(EBI3) ELISA kit

Code CSB-EL007360HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Human EBI3 ELISA Kit was designed for the quantitative measurement of Human EBI3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Name Epstein-Barr virus induced 3
Alternative Names cytokine receptor ELISA Kit; EBI3 ELISA Kit; EBV induced gene 3 protein ELISA Kit; EBV-induced gene 3 protein ELISA Kit; Epstein Barr virus induced 3 ELISA Kit; Epstein Barr virus induced gene 3 ELISA Kit; Epstein Barr virus induced gene 3 protein ELISA Kit; Epstein-Barr virus-induced gene 3 protein ELISA Kit; IL 27 subunit beta ELISA Kit; IL 27B ELISA Kit; IL-27 subunit beta ELISA Kit; IL-27B ELISA Kit; IL27 subunit ELISA Kit; IL27 subunit beta ELISA Kit; IL27B ELISA Kit; IL27B_HUMAN ELISA Kit; IL35 subunit ELISA Kit; interleukin 27 subunit beta ELISA Kit; Interleukin-27 subunit beta ELISA Kit
Abbreviation EBI3
Uniprot No. Q14213
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human EBI3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %97
Range %91-100
1:2Average %102
Range %98-105
1:4Average %86
Range %82-90
1:8Average %93
Range %88-96
The recovery of human EBI3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8984-92
EDTA plasma (n=4)10196-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
10002.643 2.589 2.616 2.476
5001.678 1.645 1.662 1.522
2501.056 1.033 1.045 0.905
1250.702 0.714 0.708 0.568
62.50.452 0.476 0.464 0.324
31.250.297 0.308 0.303 0.163
15.60.215 0.222 0.219 0.079
00.139 0.141 0.140
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
Gene References into Functions
  1. The IL-35 levels in CagA(+) H. pylori-infected participants from peptic ulcer and H. pylori-infected asymptomatic groups were lower than individuals infected with CagA(-) strains. PMID: 29938865
  2. IL-35 may be involved in the pathogenesis of Primary biliary cirrhosis. PMID: 29445068
  3. IL-35 is a relatively newly discovered member of the IL-12 family which are unique in structure as they are dimer formed by two subunits; recent findings have shown abnormal expression of IL-35 in inflammatory autoimmune diseases and functional analysis suggested that IL-35 is critical in the onset and development of these diseases. [Review] PMID: 29729445
  4. IL-35 expression was significantly increased in patients with chronic hepatitis C and was positively correlated with the levels of viral RNA PMID: 28644966
  5. Pancreatic ductal carcinoma cells produce IL35 to recruit monocytes via CCL5 and induce macrophage to promote angiogenesis via expression of CXCL1 and CXCL8. PMID: 28989066
  6. The study revealed that post-therapeutic recovery of circulating IL-35 concentration might be an independent predictor for effective response to IST in pediatric AA. PMID: 28211781
  7. The plasma levels of interleukin-35 were significantly higher in the hepatocellular carcinoma patients than the controls. PMID: 27699510
  8. this study introduces IL-35 as a new treatment for pemphigus PMID: 27855302
  9. over-expression of EBI3 could reduce the apoptosis of Treg/CD4(+)T/CD8(+)T cells and prevent radiation-induced immunosuppression of cervical cancer HeLa cells by inhibiting the activation of PD-1/PD-L1 signaling pathway PMID: 28351328
  10. Data show that interleukin-35 (Epstein-Barr virus-induced gene 3 (EBI3) and the interleukin-12 Subunit p35 (p35) subunit) levels were significantly elevated in the patients with influenza infection. PMID: 26844658
  11. higher decidual mRNA expression in preeclampsia PMID: 26472010
  12. IL-35 was elevated in bone marrow of adult AML patients and this increase was correlated with the clinical stages of malignancy, suggesting that IL-35 is involved in pathogenesis of AML. PMID: 26431888
  13. Elevated circulating IL-35, particularly at early phase, its decrease after treatment initiation, and a positive association between synovial fluid IL-35 and disease activity support an involvement of IL-35 in the pathogenesis of RA. PMID: 26204444
  14. Data suggest that the toll like receptor 3 (TLR3)-interferon regulatory factor 6 protein (IRF6)-interleukin-23 subunit p19 (p19)/EBI3 protein axis may regulate keratinocyte functions in the skin. PMID: 26303210
  15. EBI3 gene rs4740 polymorphism is closely associated with susceptibility to pulmonary tuberculosis and the elevation and enrichment of EBI3 in the lung derived from macrophages may contribute to the exacerbation of mycobacterial infection. PMID: 25937126
  16. EBI3 Downregulation Contributes to Type I Collagen Overexpression in Scleroderma Skin. PMID: 26355156
  17. IL-35 mRNA and protein were higher in tuberculous pleural effusions than in malignant ones. PMID: 25935866
  18. IL-35 can effectively suppress the proliferation and IL-4 production of activated CD4+CD25- T cells in allergic asthma, and that IL-35 may be a new immunotherapy for asthma patients. PMID: 26044961
  19. IL-35 is highly expressed in chronic HBV CD4(+) T-cells and plays an important role in the inhibition of the cellular immune response in chronic HBV. PMID: 25869609
  20. The levels of EBI3 and IL-12p35 mRNAs in peripheral blood mononuclear cells in moderate or hyper-responders were significantly higher than those in non- or hypo-responders. PMID: 25575066
  21. The results suggest that the decreased expression of IL-35 could be involved in the pathogenesis of childhood asthma. PMID: 24970690
  22. IL-17 and IL-35 may be critically involved in the pathogenesis of hepatitis B-related LC. PMID: 25323532
  23. IL35 appears to contribute to the loss of immunological self-tolerance in ITP patients by modulating T cells and immunoregulatory cytokines. PMID: 25640666
  24. IL-35 levels are dramatically decreased in immune thrombocytopenia patients, suggesting that IL-35 may be involved in the pathogenesis of this disease. PMID: 24994465
  25. Interleukin-35 induces regulatory B cells that suppress autoimmune disease. [il-35] PMID: 24743305
  26. ingestion of apoptotic cells by DCs leads to increased expression of IL-12p35 and Ebi3 without affecting IL-12p40. PMID: 24782489
  27. circulating IL-35 in PDAC patients significantly increased, suggesting that regulating the expression of IL-35 may provide a new possible target for the treatment of PDAC patients, especially for the resectable ones. PMID: 24121041
  28. The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis. PMID: 24376289
  29. EBI3-overexpression in MRL/lpr mice induces generation of regulatory T cells, causing suppression of autoimmune and inflammatory reactions by affecting the T helper (Th)1 cell/Th2 cytokine balance. PMID: 23845089
  30. in contrast to TGF-beta, IL-35 is not constitutively expressed in tissues but it is inducible in response to inflammatory stimuli PMID: 22438968
  31. the findings from the past decade identify IL-27 as a critical immunoregulatory cytokine, especially for T cells, whereas some controversy is fueled by results challenging the view of IL-27 as a classical silencer of inflammation. PMID: 23904441
  32. expressed by trophoblast cells PMID: 23619469
  33. The findings of this study suggest that SNPs in FOXP3 and EBI3 genes modify the risk for development of chronic rhinosinusitis. PMID: 23562195
  34. these results reveal a novel functional role for IL-35 in suppressing cancer activity, inhibiting cancer cell growth, and increasing the apoptosis sensitivity of human cancer cells through the regulation of genes related to the cell cycle and apoptosis. PMID: 23154182
  35. The findings of this study support the potential role of regulatory T cells and genetic variations in the regions around FOXP3 and EBI3 genes in modifying the risk for AR development in Chinese patients. PMID: 22836044
  36. This study demonstrated that interleukin-35 expression could be detected in the CD4(+) T cells from peripheral blood of chronic hepatitis B patients. PMID: 21285006
  37. IL-27 expression is one host immune factor produced in response to influenza A virus infection and that elevated IL-27 levels inhibit viral replication. PMID: 22343630
  38. Data show that Epstein-Barr virus-induced gene 3 (EBI3) is differentially expressed among Burkitt lymphoma and diffuse large B-cell lymphoma. PMID: 21931777
  39. a new heterodimeric cytokine that consists of EBI3, an IL-12p40-related protein, and p28, a newly discovered IL-12p35-related polypeptide (IL-27) PMID: 12121660
  40. results suggest that increased numbers of Epstein-Barr virus-infected cells in areas of active inflammatory bowel disease are secondary to influx or local proliferation of inflammatory cells & do not contribute significantly to local production of EBI3 PMID: 15170639
  41. findings indicate that the restricted Th1 responses in newborns owing to deficient IL-12 production may be compensated for, in part, by enhanced IL-27 secretion PMID: 18167155
  42. The genome-wide mRNA expression profile under the condition of short-term stimulation (4h) with IL-18 using the Affymetrix GeneChip((R)) Array System, was characterized. PMID: 18336908
  43. Our data support a possible role of Ebi3 in atherogenesis PMID: 19556516

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Subcellular Location Secreted.
Protein Families Type I cytokine receptor family, Type 3 subfamily
Database Links

HGNC: 3129

OMIM: 605816

KEGG: hsa:10148

STRING: 9606.ENSP00000221847

UniGene: Hs.501452

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