Code | CSB-RA256644A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
In the production of the EBI3 recombinant monoclonal antibody, in vitro expression systems are utilized, entailing the cloning of EBI3 antibody DNA sequences from immunoreactive rabbits. The immunogen used is a synthesized peptide derived from the human EBI3 protein. Subsequently, the genes encoding the EBI3 antibodies are inserted into plasmid vectors, and these recombinant plasmid vectors are transfected into host cells to enable antibody expression. The EBI3 recombinant monoclonal antibody then undergoes purification through affinity chromatography and is subjected to extensive testing in ELISA and WB applications. These tests affirm its reactivity with the human EBI3 protein.
EBI3 is a crucial subunit of the IL-27 and IL-35 cytokines, which have diverse roles in the immune system. These cytokines can have both pro-inflammatory and anti-inflammatory functions, depending on the context, and are involved in immune regulation, infection control, and the maintenance of immune tolerance.
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