Human Interleukin 5,IL-5 ELISA KIT

Instructions
Code CSB-E04636h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 5 (colony-stimulating factor, eosinophil)
Alternative Names B-cell differentiation factor I ELISA Kit; Colony stimulating factor ELISA Kit; EDF ELISA Kit; Eosinophil differentiation factor ELISA Kit; IL-5 ELISA Kit; IL5 ELISA Kit; IL5_HUMAN ELISA Kit; Interleukin 5 (colony stimulating factor; eosinophil) ELISA Kit; Interleukin 5 ELISA Kit; Interleukin-5 ELISA Kit; T Cell Replacing Factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
Abbreviation IL5
Uniprot No. P05113
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 pg/mL-50 pg/mL
Sensitivity 0.195 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 87-93  
1:2 Average % 100  
Range % 97-103  
1:4 Average % 94  
Range % 90-97  
1:8 Average % 98  
Range % 93-102  
Recovery
The recovery of human IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 92-98  
EDTA plasma (n=4) 97 94-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
50 2.771 2.745 2.758 2.630  
25 2.205 2.187 2.196 2.068  
12.5 1.352 1.374 1.363 1.235  
6.25 0.701 0.710 0.706 0.578  
3.12 0.424 0.432 0.428 0.300  
1.56 0.301 0.288 0.295 0.167  
0.78 0.214 0.206 0.210 0.082  
0 0.129 0.127 0.128    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Gene References into Functions
  1. IL-5 is expressed intrathecally in tick-borne encephalitis, but its pathogenetic role remains unclear. PMID: 29602685
  2. eosinophilia in FE is secondary to dysregulation of IL-5 production in PBMC (and their component subsets). PMID: 28226398
  3. Data indicate that interleukin-5 (IL-5) was that only serum cytokines show statistical significance between severe chronic obstructive pulmonary disease (COPD) and controls. PMID: 28398462
  4. Serum IL-5 and IL-13 are reliable biomarkers for the blood eosinophilia asthma phenotype. PMID: 27060452
  5. Longitudinal co-variations between inflammatory cytokines, lung function and patient reported outcomes in patients with asthma PMID: 28915273
  6. Obesity alters the lung neutrophil infiltration to enhance breast cancer metastasis through IL5 and GM-CSF. PMID: 28737771
  7. simvastatin was demonstrated to inhibit IL-5-induced CCR3 expression and chemotaxis of eosinophils mediated via the mevalonate pathway. PMID: 27275740
  8. Interleukin-5/-targeted treatments offer promises to patients with eosinophilic respiratory disorders; review PMID: 26859368
  9. Data indicate that interleukin 5 (IL-5)-associated single nucleotide polymorphisms (SNPs) were not associated with carotid intima-media thickness. PMID: 26821299
  10. Post-liver transplant patients with food allergy showed a unique cytokine profile in response to various stimuli, with extremely elevated IL-5, low IL-10 secretion. PMID: 26282695
  11. Production of IL-5 in response to both extract and the Bet v1-derived peptide mix strongly suggested that T cells were a major source of IL-5. PMID: 25817862
  12. It has been shown in this study that the level of IL-5, one of the markers of eosinophilic inflammation, is higher in EBC of children with atopic asthma than in those with nonatopic asthma. PMID: 25937050
  13. IL-5 may be part of protective mechanisms operating in early atherosclerosis, at least in women PMID: 25587992
  14. Results showed that PAX2 expression is significantly overexpressed in esophageal squamous cell carcinoma tissues and IL-5 is identified as PAX2's effector for metastasis. PMID: 25613757
  15. Subsequent IL-5-stimulated signaling. PMID: 25121926
  16. increased levels in recurrent versus nonrecurrent rhinosinusitis with nasal polyps at the moment of the first surgery PMID: 24980230
  17. HDM-specific IL-5 responses at age 3 years or later are the best measure of T cell function for predicting asthma at age 8 years. PMID: 24875149
  18. Fibrocytes studied were generated in vitro from PBMCs by culturing in the presence of platelet derived growth factors and stimulated by IL-33. Fibrocytes constitutively expressed IL-13 and IL-5, which was augmented by stimulation with IL-33. PMID: 24822215
  19. Mechanisms of human eosinophil migration induced by the combination of IL-5 and the endocannabinoid 2-arachidonoyl-glycerol. PMID: 24530098
  20. Patients with the 'IL-5, IL-17A, IL-25-high' airway inflammatory pattern are often uncontrolled asthmatics, despite daily treatment. PMID: 23957336
  21. These unexpected results provide a theoretical basis for the therapeutic targeting of IL-5 in bladder cancer PMID: 23770289
  22. In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
  23. When patients were classified according to sputum IgE levels, it appeared that IL-5, IL-6, IL-17 and TNF-alpha sputum supernatant levels were raised in the "IgE high" asthmatics when compared to "IgE low" asthmatics PMID: 23555579
  24. alpha(M)beta(2) integrin-mediated adhesion and motility of IL-5-stimulated eosinophils on periostin. PMID: 23306834
  25. Type I interferon (IFN)-dependent inhibition of T cell-derived IL-5 is mediated by IFN-alpha acting directly on activated T cells. PMID: 23382558
  26. Strong IL-5 production was detected in response to peptides from several of the previously undescribed proteins of grass pollen. PMID: 23401558
  27. Topical steroid treatment may suppress IL-5 gene expression, and steroids may inhibit eosinophil functions in nasal polyps. PMID: 15835818
  28. Elevated expression of inflammatory cytokines (IL-5, IL-20, and IL-28A) is associated with bladder cancer development. PMID: 22962576
  29. binding of IL-5 to IL-5Ralpha plays a critical role in MMP-9 expression, which may be involved in the migration of bladder cancer. PMID: 22710862
  30. The study reports the crystal structure of dimeric IL-5 in complex with the IL5RA extracellular domains. PMID: 22528658
  31. Elevated levels of IL-5, which uses the neural plasticity-related RAS GTPase-ERK pathway to mediate its actions in the central nervous system, could be one of the factors underlying the depression-related changes in CNS plasticity. PMID: 22230487
  32. elevated levels in induced sputum from patients with asthma and allergic rhinitis PMID: 22186238
  33. REVIEW: Interleukin-5 and IL-5 receptor in health and disease PMID: 21986312
  34. The structure demonstrates that for steric reasons, homodimeric IL-5 can bind only one receptor molecule, even though two equivalent receptor-binding sites exist. PMID: 22153509
  35. There is no correlation between the eosinophilic infiltration and the expression of IL-5 in lung cancer tissues. PMID: 21609545
  36. Transplantation of human mesenchymal stem cells suppresses middle cerebral artery occlusion (MCAO) focal ischemia-induced inflammation, possibly through expression of fractalkine and interleukin (IL)-5. PMID: 21168500
  37. results demonstrate that IL-5(+) and IL-5(-) Th2 cells, respectively, represent more and less highly differentiated Th2 cell subpopulations PMID: 21849680
  38. IL-5 may play a role in the early phase of acute pneumonia caused by the 2009 H1N1 virus in Japanese children. PMID: 21323726
  39. Activation of GATA-3 might be one of the mechanisms for induction of IL-5 expression in chronic rhinosinusitis. PMID: 17628972
  40. AP-1 may participate in the signal transduction of PKC-triggered expression of IL-5 in allergic rhinitis T lymphocytes. PMID: 17438848
  41. PPD-induced preferential secretion by PBMCs of Warao indigenous patients in Venezuela PMID: 20650294
  42. Functional polymorphisms in the genes encoding the Th2 cytokines Il-5, IL-6, and IL-13, are associated differently with the development and prognosis of autoimmune thyroid disease from each other. PMID: 21235536
  43. IL-5 and IL-8 are the key cytokines in the formation of nasal polyps. PMID: 16929824
  44. Suggest that IL-5 responses in cord blood samples were strongly related to the season of birth. PMID: 20825427
  45. promotes increased immunoglobulin M at the site of disease in leprosy PMID: 20561085
  46. Our study identifies CXCL10 and IL-5 as proteins differentiating complicated and uncomplicated plaques from human carotid arteries PMID: 20943047
  47. Sputum IL-5 was associated with a sputum eosinophilia. PMID: 19478474
  48. data indicate that ability to produce the type 2 cytokines IL-13 and IL-5 defines CD161(+) NK cells at intermediate stages of differentiation, and is lost upon terminal functional differentiation, concomitant with acquired ability to produce IFN-gamma PMID: 11830476
  49. CD4(+) T cells are the major source of IL-5 among CD3(+) lymphocytes in atopic asthmatic subjects, whereas in nonatopic asthmatic subjects CD8 (+) T cells as well as CD4(+) T cells contribute to the increased production of IL-5 PMID: 11842300
  50. By itself, IL5 increases nerve growth factor level in eosinophils, but in combination with immune complexes, significantly reduces eosinophil NGF levels. PMID: 11877300

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Subcellular Location Secreted
Protein Families IL-5 family
Database Links

HGNC: 6016

OMIM: 147850

KEGG: hsa:3567

STRING: 9606.ENSP00000231454

UniGene: Hs.2247

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