Code | CSB-E04636h |
Size | 96T,5×96T,10×96T |
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Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 88 | ||||
Range % | 82-96 | |||||
1:2 | Average % | 92 | ||||
Range % | 90-105 | |||||
1:4 | Average % | 95 | ||||
Range % | 92-103 | |||||
1:8 | Average % | 90 | ||||
Range % | 86-98 |
The recovery of human IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 85 | 80-92 | ||||
EDTA plasma (n=4) | 87 | 82-94 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
250 | 2.973 | 2.834 | 2.904 | 2.754 | |||
125 | 2.276 | 2.167 | 2.222 | 2.072 | |||
62.5 | 1.248 | 1.228 | 1.238 | 1.089 | |||
31.25 | 0.635 | 0.629 | 0.632 | 0.483 | |||
15.6 | 0.363 | 0.371 | 0.367 | 0.218 | |||
7.8 | 0.269 | 0.253 | 0.261 | 0.112 | |||
3.9 | 0.217 | 0.214 | 0.216 | 0.066 | |||
0 | 0.144 | 0.155 | 0.149 |
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The human IL-5 ELISA kit is suitable for the quantitative determination of human IL-5 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.
IL-5 is a homodimeric cytokine responsible for the maturation of eosinophils in the bone marrow and their release into the blood. In humans, IL-5 functions exclusively on eosinophils and basophils and induces their maturation, growth, activation, and survival. In allergies, IL-5 does not take part in IgE synthesis rather than acting on mast cells to promote histamine release. IL-5 increases the expression of IL-2 receptors on B-cells and promotes B-cell proliferation and differentiation. IL-5 is associated with the pathogenesis of atopic diseases.
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