Human Interleukin 5,IL-5 ELISA KIT

Code CSB-E04636h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P05113
Lead Time 3-5 working days
Abbreviation IL5
Target Name interleukin 5 (colony-stimulating factor, eosinophil)
Alias EDF, IL-5, TRF, B cell differentiation factor I|T-cell replacing factor|eosinophil differentiation factor|interleukin 5|interleukin-5
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 pg/mL-50 pg/mL
Sensitivity 0.195 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
HGNC 6016
RGD 2900
MGI 96557
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 87-93  
1:2 Average % 100  
Range % 97-103  
1:4 Average % 94  
Range % 90-97  
1:8 Average % 98  
Range % 93-102  
Recovery
The recovery of human IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 92-98  
EDTA plasma (n=4) 97 94-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
50 2.771 2.745 2.758 2.630  
25 2.205 2.187 2.196 2.068  
12.5 1.352 1.374 1.363 1.235  
6.25 0.701 0.710 0.706 0.578  
3.12 0.424 0.432 0.428 0.300  
1.56 0.301 0.288 0.295 0.167  
0.78 0.214 0.206 0.210 0.082  
0 0.129 0.127 0.128    
References
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Function Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Subcellular Location Secreted
Protein Families IL-5 family
Database Links

HGNC: 6016

OMIM: 147850

KEGG: hsa:3567

STRING: 9606.ENSP00000231454

UniGene: Hs.2247

Pathway Jak-STAT signaling pathway
Fc epsilon RI signaling pathway
Hematopoietic cell lineage
IL-17 signaling pathway
T cell receptor signaling pathway
Th1 and Th2 cell differentiation

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