Interleukin-5 (IL-5) is a cytokine that regulates eosinophil biology and B-cell differentiation. Originally identified as a B-cell differentiation factor and T-cell replacing factor, IL-5 is primarily produced by activated T-helper 2 cells and serves as a key mediator in allergic and inflammatory responses. The cytokine promotes eosinophil development, activation, and survival, making it an important factor in asthma, allergic rhinitis, and other eosinophil-associated disorders. IL-5 also functions as an eosinophil differentiation factor, regulating the maturation and recruitment of these cells to sites of inflammation.
This Human Interleukin-5 ELISA kit uses a quantitative sandwich measurement principle to detect IL-5 in serum, plasma, and tissue homogenates. The assay offers a detection range of 3.9 pg/ml to 250 pg/ml with a sensitivity of 0.97 pg/ml. The protocol requires 50-100 μl sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for measuring human IL-5 levels in various biological samples.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research examining inflammatory and immune responses, particularly in studies analyzing cytokine profiles in various disease contexts. The kit allows quantitative measurement of IL-5 in human biological samples, supporting research into eosinophil-mediated inflammatory processes and immune system dysregulation. Applications span from serum-based biomarker studies to tissue-based protein expression analysis.
• Inflammatory biomarker research: Serum-based studies measuring IL-5 alongside other inflammatory cytokines to assess immune system activation and cytokine network interactions in disease states
• Cytokine profiling studies: Multi-analyte studies examining various interleukins and immune mediators to characterize inflammatory responses and immune cell differentiation patterns
• Tissue-based protein analysis: Quantification of IL-5 protein levels in tissue homogenates from esophageal samples, allowing assessment of local inflammatory responses in specific anatomical sites
• Immune system research: Studies examining T-cell and B-cell mediated responses, particularly focusing on eosinophil differentiation and activation pathways in various pathological conditions
