Recombinant Human Interleukin-5 protein(IL5) (Active)

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Code CSB-AP001721HU
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.
Target Names IL5
Uniprot No. P05113
Research Area Immunology
Alternative Names B-cell differentiation factor I; Colony stimulating factor; EDF; Eosinophil differentiation factor; IL-5; IL5; IL5_HUMAN; Interleukin 5 (colony stimulating factor; eosinophil); Interleukin 5; Interleukin-5; T Cell Replacing Factor; T-cell replacing factor; TRF
Species Homo sapiens (Human)
Source E.Coli
Expression Region 20-134aa
Complete Sequence M+IPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES
Mol. Weight 13.3 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a a 0.2 µm filtered 20mM PB, pH 7.5, 3 % trehalose, 0.1 % Tween-80
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Gene References into Functions
  1. IL-5 is expressed intrathecally in tick-borne encephalitis, but its pathogenetic role remains unclear. PMID: 29602685
  2. eosinophilia in FE is secondary to dysregulation of IL-5 production in PBMC (and their component subsets). PMID: 28226398
  3. Data indicate that interleukin-5 (IL-5) was that only serum cytokines show statistical significance between severe chronic obstructive pulmonary disease (COPD) and controls. PMID: 28398462
  4. Serum IL-5 and IL-13 are reliable biomarkers for the blood eosinophilia asthma phenotype. PMID: 27060452
  5. Longitudinal co-variations between inflammatory cytokines, lung function and patient reported outcomes in patients with asthma PMID: 28915273
  6. Obesity alters the lung neutrophil infiltration to enhance breast cancer metastasis through IL5 and GM-CSF. PMID: 28737771
  7. simvastatin was demonstrated to inhibit IL-5-induced CCR3 expression and chemotaxis of eosinophils mediated via the mevalonate pathway. PMID: 27275740
  8. Interleukin-5/-targeted treatments offer promises to patients with eosinophilic respiratory disorders; review PMID: 26859368
  9. Data indicate that interleukin 5 (IL-5)-associated single nucleotide polymorphisms (SNPs) were not associated with carotid intima-media thickness. PMID: 26821299
  10. Post-liver transplant patients with food allergy showed a unique cytokine profile in response to various stimuli, with extremely elevated IL-5, low IL-10 secretion. PMID: 26282695
  11. Production of IL-5 in response to both extract and the Bet v1-derived peptide mix strongly suggested that T cells were a major source of IL-5. PMID: 25817862
  12. It has been shown in this study that the level of IL-5, one of the markers of eosinophilic inflammation, is higher in EBC of children with atopic asthma than in those with nonatopic asthma. PMID: 25937050
  13. IL-5 may be part of protective mechanisms operating in early atherosclerosis, at least in women PMID: 25587992
  14. Results showed that PAX2 expression is significantly overexpressed in esophageal squamous cell carcinoma tissues and IL-5 is identified as PAX2's effector for metastasis. PMID: 25613757
  15. Subsequent IL-5-stimulated signaling. PMID: 25121926
  16. increased levels in recurrent versus nonrecurrent rhinosinusitis with nasal polyps at the moment of the first surgery PMID: 24980230
  17. HDM-specific IL-5 responses at age 3 years or later are the best measure of T cell function for predicting asthma at age 8 years. PMID: 24875149
  18. Fibrocytes studied were generated in vitro from PBMCs by culturing in the presence of platelet derived growth factors and stimulated by IL-33. Fibrocytes constitutively expressed IL-13 and IL-5, which was augmented by stimulation with IL-33. PMID: 24822215
  19. Mechanisms of human eosinophil migration induced by the combination of IL-5 and the endocannabinoid 2-arachidonoyl-glycerol. PMID: 24530098
  20. Patients with the 'IL-5, IL-17A, IL-25-high' airway inflammatory pattern are often uncontrolled asthmatics, despite daily treatment. PMID: 23957336
  21. These unexpected results provide a theoretical basis for the therapeutic targeting of IL-5 in bladder cancer PMID: 23770289
  22. In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
  23. When patients were classified according to sputum IgE levels, it appeared that IL-5, IL-6, IL-17 and TNF-alpha sputum supernatant levels were raised in the "IgE high" asthmatics when compared to "IgE low" asthmatics PMID: 23555579
  24. alpha(M)beta(2) integrin-mediated adhesion and motility of IL-5-stimulated eosinophils on periostin. PMID: 23306834
  25. Type I interferon (IFN)-dependent inhibition of T cell-derived IL-5 is mediated by IFN-alpha acting directly on activated T cells. PMID: 23382558
  26. Strong IL-5 production was detected in response to peptides from several of the previously undescribed proteins of grass pollen. PMID: 23401558
  27. Topical steroid treatment may suppress IL-5 gene expression, and steroids may inhibit eosinophil functions in nasal polyps. PMID: 15835818
  28. Elevated expression of inflammatory cytokines (IL-5, IL-20, and IL-28A) is associated with bladder cancer development. PMID: 22962576
  29. binding of IL-5 to IL-5Ralpha plays a critical role in MMP-9 expression, which may be involved in the migration of bladder cancer. PMID: 22710862
  30. The study reports the crystal structure of dimeric IL-5 in complex with the IL5RA extracellular domains. PMID: 22528658
  31. Elevated levels of IL-5, which uses the neural plasticity-related RAS GTPase-ERK pathway to mediate its actions in the central nervous system, could be one of the factors underlying the depression-related changes in CNS plasticity. PMID: 22230487
  32. elevated levels in induced sputum from patients with asthma and allergic rhinitis PMID: 22186238
  33. REVIEW: Interleukin-5 and IL-5 receptor in health and disease PMID: 21986312
  34. The structure demonstrates that for steric reasons, homodimeric IL-5 can bind only one receptor molecule, even though two equivalent receptor-binding sites exist. PMID: 22153509
  35. There is no correlation between the eosinophilic infiltration and the expression of IL-5 in lung cancer tissues. PMID: 21609545
  36. Transplantation of human mesenchymal stem cells suppresses middle cerebral artery occlusion (MCAO) focal ischemia-induced inflammation, possibly through expression of fractalkine and interleukin (IL)-5. PMID: 21168500
  37. results demonstrate that IL-5(+) and IL-5(-) Th2 cells, respectively, represent more and less highly differentiated Th2 cell subpopulations PMID: 21849680
  38. IL-5 may play a role in the early phase of acute pneumonia caused by the 2009 H1N1 virus in Japanese children. PMID: 21323726
  39. Activation of GATA-3 might be one of the mechanisms for induction of IL-5 expression in chronic rhinosinusitis. PMID: 17628972
  40. AP-1 may participate in the signal transduction of PKC-triggered expression of IL-5 in allergic rhinitis T lymphocytes. PMID: 17438848
  41. PPD-induced preferential secretion by PBMCs of Warao indigenous patients in Venezuela PMID: 20650294
  42. Functional polymorphisms in the genes encoding the Th2 cytokines Il-5, IL-6, and IL-13, are associated differently with the development and prognosis of autoimmune thyroid disease from each other. PMID: 21235536
  43. IL-5 and IL-8 are the key cytokines in the formation of nasal polyps. PMID: 16929824
  44. Suggest that IL-5 responses in cord blood samples were strongly related to the season of birth. PMID: 20825427
  45. promotes increased immunoglobulin M at the site of disease in leprosy PMID: 20561085
  46. Our study identifies CXCL10 and IL-5 as proteins differentiating complicated and uncomplicated plaques from human carotid arteries PMID: 20943047
  47. Sputum IL-5 was associated with a sputum eosinophilia. PMID: 19478474
  48. data indicate that ability to produce the type 2 cytokines IL-13 and IL-5 defines CD161(+) NK cells at intermediate stages of differentiation, and is lost upon terminal functional differentiation, concomitant with acquired ability to produce IFN-gamma PMID: 11830476
  49. CD4(+) T cells are the major source of IL-5 among CD3(+) lymphocytes in atopic asthmatic subjects, whereas in nonatopic asthmatic subjects CD8 (+) T cells as well as CD4(+) T cells contribute to the increased production of IL-5 PMID: 11842300
  50. By itself, IL5 increases nerve growth factor level in eosinophils, but in combination with immune complexes, significantly reduces eosinophil NGF levels. PMID: 11877300
  51. in asthma Il-5 might directly induce bronchial hyperresponsiveness PMID: 11897983
  52. A putative Bcl6-binding silencer element has been identified in the 3' untranslated region of IL-5 cDNA. PMID: 12097386
  53. the AP-1 complex plays a key role in regulating IL5 expression- this provides an explanation for the sensitivity of IL5 to protein synthesis inhibitors and a mechanism for the specific induction of IL5 compared with other cytokines. PMID: 12354764
  54. Constitutive overexpression of human IL-5 in transgenic mice induces migration of bone marrow progenitor cells to the spleen and extramedullary hematopoiesis in the spleen. PMID: 12393708
  55. IL-5 down-modulates its receptor via a proteinase-mediated process. PMID: 12444155
  56. Increased IL-5 expression in muscle of eosinophilic myositis patients correlates with the presence of activated eosinophilic granules and the release of eosinophilic major basic protein in muscle biopsy specimens. PMID: 12534990
  57. This is the first evidence of an association between the IL5 gene polymorphism and bronchial asthma PMID: 12575459
  58. Incubation of eosinophils with IL-5 leads to reduced expression of IL-5R alpha, which is sustained for up to 5 days; eosinophil IL-3R alpha expression is increased by IL-5, whereas GM-CSF receptor alpha expression is not affected by IL-5. PMID: 12759409
  59. IL-5 gene transcription in human asthmatic peripheral T cells in vivo clearly demonstrate that an interaction with monocytes enhances IL-5 gene transcription. PMID: 12771545
  60. Results show that the expression of interleukin-5 mRNA in mild asthma differed from that in severe and moderate asthma before and after corticosteroid treatment. PMID: 12910312
  61. IL-5 and eotaxin are associated with acute exacerbation of asthma. PMID: 12915771
  62. IL5 gene may play a role in blood eosinophilia associated with atopic dermatitis PMID: 14581138
  63. Results identify several transcriptional targets of interleukin-5 and granulocyte macrophage-colony-stimulating factor in human eosinophils and suggest that a number of protein products are critical to the responsiveness of airway eosinophils. PMID: 14630612
  64. Significantly higher levels were found in soluble egg antigen-stimulated PBMC from schistosomiasis mansoni patients with degree III hepatic fibrosis compared to patients with degree I or II fibrosis, and in patients untreated for 1 year. PMID: 15155645
  65. histone acetyltransferase (HAT) activity of CBP/p300 was required to activate IL-5 expression PMID: 15271374
  66. IL-5 induces the protein expression of cyclin D3 and the kinase Pim-1, both of which are regulated by STAT-dependent processes in airway and blood eosinophils. PMID: 15528381
  67. IL-5 acts as a triggering factor in the toxiallergic complaints commonly seen in helminth and protozoon infections. PMID: 15534922
  68. Up-regulated by a conserved upstream enhancer with two potential GATA-3-binding sites PMID: 15549733
  69. extracellular signal-regulated kinase-mediated adhesion of beta(2)-integrin caused by IL-5 is mediated in human eosinophils by a class IA PI3K through activation of a PKCdelta pathway PMID: 15802551
  70. analysis of IL5 induction of activation of the multisubunit receptor system using an interleukin-5 mimetic peptide PMID: 15826943
  71. interleukin-5 transcription repression by the glucocorticoid receptor targets GATA3 signaling and involves histone deacetylase recruitment PMID: 15826950
  72. Allergen-specific T cell lines induced in the presence of salmeterol and fluticasone proprionate inhibited IL-5 and IL-13 production by allergen-specific Th2 cell lines in an IL-10-dependent manner PMID: 15845862
  73. Interleukin 5 plays a role in colonic carcinogenesis from ulcerative colitis by interacting with IGF-II. PMID: 15935984
  74. Polymorphisms in the IL5 gene were associated with each other in whites and African Americans. PMID: 15951665
  75. This Th2 cytokine is possibly involved in the modulation of lung graft transplantation tolerance. PMID: 15964392
  76. Eosinophils from asthmatics release IL-5 in an autocrine fashion to act on their own IL-5 receptors in prevention of apoptosis through the upregulation of Bcl-2 expression. PMID: 16036415
  77. Sputum IL-13, but not IL-5, is inversely correlated with the provocative concentration of a substance causing a 20% fall in FEV1 for methacholine in asthmatic patients PMID: 16236836
  78. The conserved lymphokine element 0 is important for inducible interleukin-5 transcription. PMID: 16243713
  79. dynamin has roles in the IL-5 signaling pathway and in receptor endocytosis and termination of the ERK1/2 signaling pathway PMID: 16556602
  80. serum expression of eotaxin & IL-5 were significantly increased in patients with strongyloidiasis compared with controls; this rise suggests that selective mediators of the eosinophil can have a role in immunity against S. stercoralis in human infection PMID: 16879311
  81. HDAC4 (histone deacetylase 4) and p300, a known HAT (histone acetyltransferase), reversibly controlled the activity of the IL-5 promoter in vivo and in vitro, with a concurrent alteration of histone H3 acetylation status at the promoter regions. PMID: 16922677
  82. Ragweed stimulation significantly increased the production of the Th2-associated cytokines IL-5, IL-9 and IL-13, the chemokines CCL17 and CCL22 and the regulatory cytokine IL-10 in allergic patients PMID: 17517104
  83. Inhaled IL-5 modulated eosinophil progenitor numbers in both the airways and bone marrow of asthmatics and induced local eosinophilia in non-asthmatics. PMID: 17581195
  84. A significant correlation exists between bronchoalveolar lavage concentration of IL-5 and eosinophils in veterans with sulfur mustard gas-induced pulmonary fibrosis. PMID: 17620002
  85. IL-5 priming enhances Siglec-8-mediated mitochondrial and ROS-dependent eosinophil apoptosis and eliminates caspase dependence. PMID: 17690326
  86. Chromatin immunoprecipitation showed increased binding of Ets1 and GATA-3 to the IL-5 promoter after stimulation. Ets1, GATA-3 and AP-1 synergize to regulate IL-5 transcription in human T cells. PMID: 17845581
  87. Glucocorticoid receptor beta is able to act as a transcriptional repressor of cytokine genes, IL5 and IL13 and mediates its function through the recruitment of histone deacetylase complexes. PMID: 18028994
  88. IL-5 may modulate the immune response in individuals with symptomatic Schistosomiasis japonica. PMID: 18056382
  89. thrombin and cell interaction with lung epithelial cells may augment the inflammatory response in allergic diseases by stimulating the secretion of IL-5 from basophils PMID: 18206982
  90. ability of CD8hi CD57+ T cells to further differentiate is highlighted by a distinct cytokine profile late after activation that includes the unexpected release of high levels of interleukin PMID: 18383036
  91. IL-5 overexpression is associated with eosinophilia in T-cell lymphomas. PMID: 18395252
  92. Reduction of IL-5, which is involved in regulating B cell differentiation into antibody-secreting plasma cells, may contribute to defective antibody production in common variable immunodeficiency patients. PMID: 18686101
  93. Alterations of MMP-2 and -9 expression may play a role in eosinophilic chronic rhinosinusitis, but the association with IL-5 and IL-13 remains unclear PMID: 18712166
  94. cyclic AMP signals enhance histone H3 acetylation at the IL-5 promoter and the concerted binding of GATA-3 and NFATc to the promoter. PMID: 18772129
  95. Analysis of IL-5 expression suggests that several cytokines could participate in the physiopathology of EE in humans.IL-5 was not detected in control samples, and expression levels were variably downregulated after steroid treatment in six patients. PMID: 18844613
  96. Diffuse large B-cell lymphoma and follicular lymphoma grade 3 is more influenced by expression of B-cell differentiation stage genes than by tumor cells growth pattern, BCL2 and BCL6 abnormalities, and International Prognostic Index. PMID: 18925696
  97. Children with the higher IL-10/IL-5 ratios appeared to have lower risks for virtually all of the four categories of respiratory infections in each of the five 1-year periods spanning the study. PMID: 18996999
  98. Pim-1, but not PI3K, plays a major role in IL-5-mediated antiapoptotic signaling in eosinophils. PMID: 19152965
  99. IL-5 induces cell proliferation and anti-apoptosis through the JAK/c-Myc pathway, and JAK1 and JAK2 activation participate in IL-5-induced up-regulation of c-Myc. PMID: 19180571
  100. PPARalpha was demonstrated, for the first time, to regulate the IL-4 and IL-5 genes in experimental autoimmune encephalomyelitis PMID: 19299749

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Subcellular Location Secreted
Protein Families IL-5 family
Database Links

HGNC: 6016

OMIM: 147850

KEGG: hsa:3567

STRING: 9606.ENSP00000231454

UniGene: Hs.2247

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