Name: Human Interleukin17B (IL17B) ELISA Kit
Brand: CUSABIO
SKU: CSB-E15918h

Product Details

Target Name

interleukin 17B

Alternative Names

Cytokine like protein Zcyto7 ELISA Kit; Cytokine Zcyto7 ELISA Kit; IL 17B ELISA Kit; IL 20 ELISA Kit; IL-17B ELISA Kit; IL-20 ELISA Kit; IL17B ELISA Kit; IL17B_HUMAN ELISA Kit; IL20 ELISA Kit; Interleukin 17 beta ELISA Kit; Interleukin 17B ELISA Kit; Interleukin 20 ELISA Kit; Interleukin-17B ELISA Kit; Interleukin-20 ELISA Kit; MGC138900 ELISA Kit; MGC138901 ELISA Kit; Neuronal interleukin 17 related factor ELISA Kit; Neuronal interleukin-17-related factor ELISA Kit; NIRF ELISA Kit; ZCYTO7 ELISA Kit

Abbreviation

IL17B

Uniprot No.

Q9UHF5

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL17B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	103-109
1:2	Average %	93
	Range %	92-98
1:4	Average %	92
	Range %	86-96
1:8	Average %	94
	Range %	89-97

Recovery

The recovery of human IL17B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-109
EDTA plasma (n=4)	92	87-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.066	2.122	2.094	1.974
400	1.574	1.625	1.600	1.480
200	1.082	1.113	1.098	0.978
100	0.643	0.687	0.665	0.545
50	0.391	0.406	0.399	0.279
25	0.256	0.255	0.256	0.136
12.5	0.211	0.221	0.216	0.096
0	0.119	0.121	0.120

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Background

Function (From Uniprot)	Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.
Gene References into Functions	The results indicate that the IL-17B/IL-17RB signaling can promote the growth and migration of tumor cells. PMID: 27146881 Our data revealed a new mechanism that IL-17B enhanced the progression of gastric cancer by activating mesenchymal stem cells. PMID: 28145881 Segregation analysis revealed that variants c.475T>G in SKP1, c.671G>A in PROB1, and c.527G>A in IL17B in the 5q31.1-q35.3 linkage region, and c.850G>A in HKDC1 in the 10q22 locus completely segregated with the phenotype in the studied Keratoconus family PMID: 27703147 This comprehensive review details the recognition of activity, signaling, and the roles of IL17B-IL17RB in breast cancer have caused to determination of new therapeutic mechanisms--{REVIEW} PMID: 28160754 Studies indicate that interleukin 17 (IL-17B)/interleukin 17 receptor B (IL-17RB) signaling is essential for pancreatic cancer malignancy. PMID: 26285835 IL-17B-IL-17RB signaling promotes pancreatic cancer malignancy. PMID: 25732306 Results show that amplified signaling of interleukin-17 receptor B (IL-17RB) and its ligand IL-17B promot tumorigenicity in breast cancer cells and impeded acinus formation in immortalized normal mammary epithelial cells. PMID: 23851503 IL-17B can enhance the effects of TNF-alpha on the production of cytokines and chemokines that control immune cell trafficking and neutrophil homeostasis in the inflamed tissues. PMID: 24056520 IL-17A, IL-17B, IL-17F and IL-23 in systemic lupus erythematosus patients were examined and the correlation between levels of the investigated cytokines and VEGF, PIGF, as well as number of endothelial cells, was investigated. PMID: 23661335 expression and functional study of IL-17B PMID: 22394632 IL-17 may play an important role in the occurrence of nasal polyps by overexpression. PMID: 16805377 Show More Hide All
Subcellular Location	Secreted.
Protein Families	IL-17 family
Tissue Specificity	Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.
Database Links	HGNC: 5982 OMIM: 604627 KEGG: hsa:27190 STRING: 9606.ENSP00000261796 UniGene: Hs.156979

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Human Interleukin17B (IL17B) ELISA Kit

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Code	CSB-E15918h
Size	96T,5×96T,10×96T
Trial Size
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