Recombinant Human Interleukin-17B protein (IL17B) gets expressed in E. coli and represents the full mature protein spanning amino acids 21 to 180. This tag-free preparation shows purity greater than 95% when verified by SDS-PAGE. The product appears to demonstrate full biological activity, with an ED50 of less than 1.0 μg/ml in IL-8 secretion assays on human HepG2 cells. This translates to a specific activity of over 1000 IU/mg. Endotoxin levels stay below 1.0 EU/μg according to the LAL method.
Interleukin-17B belongs to the IL-17 cytokine family, which is known for its involvement in immune responses. It likely plays a role in inflammatory pathways and has been studied for its effects on immune cell signaling. The protein is commonly used in research settings to explore mechanisms of inflammation and immune regulation, potentially offering insights into therapeutic targets for inflammatory conditions.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. IL-8 Induction Assays for Inflammatory Response Studies
This recombinant IL-17B protein can be used to study inflammatory signaling pathways through its demonstrated ability to induce IL-8 secretion in human HepG2 cells. The defined ED50 of less than 1.0 μg/ml provides what appears to be a reliable dose-response parameter for experimental design. Researchers can use this protein to investigate downstream inflammatory mediators and cytokine cascades that IL-17B signaling may trigger. The high purity (>95%) and low endotoxin levels should ensure minimal interference from contaminants in cell-based assays.
2. Receptor Binding and Interaction Studies
The biologically active IL-17B protein can serve as a ligand in receptor binding assays to characterize IL-17B receptor interactions and binding kinetics. The tag-free nature of this protein likely eliminates potential steric hindrance that could affect natural receptor binding. Researchers can use this protein in surface plasmon resonance, biolayer interferometry, or competitive binding assays to determine binding affinities and kinetic parameters. The mature protein sequence (21-180aa) represents what is presumably the physiologically relevant form for receptor interaction studies.
3. Antibody Development and Validation
This recombinant IL-17B protein can work as an immunogen for generating specific antibodies or as a standard for antibody characterization and validation. The high purity and biological activity appear to make it suitable for immunization protocols and subsequent antibody screening assays. Researchers can apply this protein in ELISA development, Western blot validation, and specificity testing of IL-17B-targeting antibodies. The defined specific activity (>1000 IU/mg) provides a quantitative reference for antibody potency assessments.
4. Cytokine Network Analysis in Hepatocyte Models
Given the demonstrated activity on HepG2 hepatocyte cells, this IL-17B protein may be useful for studying liver-specific inflammatory responses and cytokine networks. Researchers can investigate what role IL-17B might play in hepatic inflammation models and how it interacts with other cytokines in liver cell systems. The protein can be used to examine dose-dependent responses and temporal patterns of cytokine expression in hepatocyte cultures. The low endotoxin content suggests that observed effects are specifically attributable to IL-17B activity rather than bacterial contaminants.
5. Signal Transduction Pathway Mapping
This biologically active IL-17B protein can be applied to map and characterize intracellular signaling pathways that IL-17B receptor engagement activates. Researchers can use this protein to study phosphorylation cascades, transcription factor activation, and gene expression changes following IL-17B stimulation. The defined biological activity allows for consistent experimental conditions when investigating pathway components and their temporal activation patterns. Time-course studies using this protein may help elucidate the sequence of molecular events in IL-17B-mediated cellular responses.
