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Recombinant Human Interleukin-17B protein (IL17B) (Active)

Recombinant Human Interleukin-17B protein (IL17B) (Active)

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Code	CSB-AP001651HU
Abbreviation	Recombinant Human IL17B protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Product Details
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Target Background

Product Details

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion of human HepG2 cells is less than 1.0 μg/ml, corresponding to a specific activity of >1000 IU/mg.

Target Names

IL17B

Uniprot No.

Q9UHF5

Research Area

Immunology

Alternative Names

Cytokine like protein Zcyto7; Cytokine Zcyto7; IL 17B; IL 20 ; IL-17B; IL-20; IL17B; IL17B_HUMAN; IL20 ; Interleukin 17 beta; Interleukin 17B; Interleukin 20; Interleukin-17B; Interleukin-20; MGC138900; MGC138901; Neuronal interleukin 17 related factor ; Neuronal interleukin-17-related factor; NIRF ; ZCYTO7

Species

Homo sapiens (Human)

Source

E.Coli

Expression Region

21-180aa

Complete Sequence

M+QPRSPKSKR KGQGRPGPLA PGPHQVPLDL VSRMKPYARM EEYERNIEEM VAQLRNSSEL AQRKCEVNLQ LWMSNKRSLS PWGYSINHDP SRIPVDLPEA RCLCLGCVNP FTMQEDRSMV SVPVFSQVPV RRRLCPPPPR TGPCRQRAVM ETIAVGCTCI F

Mol. Weight

18.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4, with 0.1 % Tween-20 and 3 % Trehalose

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

IL-17B signals through the IL-17RB receptor to modulate innate immune responses, yet studying its biology requires a protein preparation with verified potency and minimal endotoxin contamination to avoid LPS-driven artifacts in immune cell assays. This tag-free recombinant human IL-17B, encompassing the full mature sequence (residues 21–180), induces IL-8 secretion in HepG2 cells with an ED50 below 1.0 μg/ml (specific activity >1,000 IU/mg), confirming signaling competence suitable for NF-κB and MAPK pathway dissection, cell-based activation assays, and use as a reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg satisfy the stringent criteria typical for primary immune cell cultures and in vivo inflammation models, while purity exceeding 95% by SDS-PAGE supports reliable dose–response characterization and antibody development workflows including immunization, ELISA coating, and western blot positive controls.

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Target Background

Function

Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.

Gene References into Functions

The results indicate that the IL-17B/IL-17RB signaling can promote the growth and migration of tumor cells. PMID: 27146881
Our data revealed a new mechanism that IL-17B enhanced the progression of gastric cancer by activating mesenchymal stem cells. PMID: 28145881
Segregation analysis revealed that variants c.475T>G in SKP1, c.671G>A in PROB1, and c.527G>A in IL17B in the 5q31.1-q35.3 linkage region, and c.850G>A in HKDC1 in the 10q22 locus completely segregated with the phenotype in the studied Keratoconus family PMID: 27703147
This comprehensive review details the recognition of activity, signaling, and the roles of IL17B-IL17RB in breast cancer have caused to determination of new therapeutic mechanisms--{REVIEW} PMID: 28160754
Studies indicate that interleukin 17 (IL-17B)/interleukin 17 receptor B (IL-17RB) signaling is essential for pancreatic cancer malignancy. PMID: 26285835
IL-17B-IL-17RB signaling promotes pancreatic cancer malignancy. PMID: 25732306
Results show that amplified signaling of interleukin-17 receptor B (IL-17RB) and its ligand IL-17B promot tumorigenicity in breast cancer cells and impeded acinus formation in immortalized normal mammary epithelial cells. PMID: 23851503
IL-17B can enhance the effects of TNF-alpha on the production of cytokines and chemokines that control immune cell trafficking and neutrophil homeostasis in the inflamed tissues. PMID: 24056520
IL-17A, IL-17B, IL-17F and IL-23 in systemic lupus erythematosus patients were examined and the correlation between levels of the investigated cytokines and VEGF, PIGF, as well as number of endothelial cells, was investigated. PMID: 23661335
expression and functional study of IL-17B PMID: 22394632
IL-17 may play an important role in the occurrence of nasal polyps by overexpression. PMID: 16805377

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Subcellular Location

Secreted.

Protein Families

IL-17 family

Tissue Specificity

Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Database Links

HGNC: 5982

OMIM: 604627

KEGG: hsa:27190

STRING: 9606.ENSP00000261796

UniGene: Hs.156979

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