Human Krueppel-like factor 4(KLF4) ELISA kit

Code CSB-EL012394HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O43474
Lead Time 5-7 working days
Abbreviation KLF4
Protein Biological Process 1 Transcription/Transcription regulation
Target Name Kruppel-like factor 4 (gut)
Alias EZF, GKLF, endothelial Kruppel-like zinc finger protein
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 6348
RGD 621445
MGI 1342287
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human KLF4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 85-92  
1:2 Average % 102  
Range % 97-107  
1:4 Average % 85  
Range % 90-100  
1:8 Average % 97  
Range % 91-103  
The recovery of human KLF4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 83 80-86  
EDTA plasma (n=4) 98 95-101  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1200 2.478 2.498 2.488 2.304  
600 2.015 2.136 2.076 1.892  
300 1.412 1.465 1.439 1.255  
150 0.768 0.758 0.763 0.579  
75 0.498 0.472 0.485 0.301  
37.5 0.368 0.355 0.362 0.178  
18.75 0.271 0.282 0.277 0.093  
0 0.183 0.185 0.184    
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Function Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Subcellular Location Nucleus
Protein Families Krueppel C2H2-type zinc-finger protein family
Database Links

HGNC: 6348

OMIM: 602253

KEGG: hsa:9314

UniGene: Hs.376206

Pathway Signaling pathways regulating pluripotency of stem cells

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